################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI AspEI AviII AvrII BbeI BbrPI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SalI SapI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AocI 2191 CCTGAGG CC^T[ACGT]AGG Asp700I 1578 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1222 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2186 GGATCC G^GATCC BcgI 1240 CGAGCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1606 CTAG C^TAG BglI 1275 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 1177 GAGACC GGTCTC(1/5) BspHI 1146 TCATGA T^CATGA BsrBI 891 GAGCGG CCGCTC(-3/-3) BsrDI 1035 CATTGC GCAATG(2/0) BsrFI 3 GCCGGC [GA]^CCGG[CT] BssSI 658 CTCGTG CACGAG(-5/-1) BstEII 310 GGTGACC G^GT[ACGT]ACC BstPI 310 GGTGACC G^GT[ACGT]ACC Bsu36I 2191 CCTGAGG CC^T[ACGT]AGG Cfr10I 3 GCCGGC [GA]^CCGG[CT] DraI 1303 TTTAAA TTT^AAA DrdI 775 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 841 CTCTTC CTCTTC(1/4) Eco81I 2191 CCTGAGG CC^T[ACGT]AGG EcoNI 1847 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 310 GGTGACC G^GT[ACGT]ACC EcoT22I 1435 ATGCAT ATGCA^T Ksp632I 841 CTCTTC CTCTTC(1/4) KspI 861 CCGCGG CCGC^GG MaeI 1606 CTAG C^TAG NaeI 3 GCCGGC GCC^GGC NgoMIV 3 GCCGGC G^CCGGC NsiI 1435 ATGCAT ATGCA^T PflFI 1222 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1146 TCATGA T^CATGA SacI 652 GAGCTC GAGCT^C SacII 861 CCGCGG CCGC^GG ScaI 1673 AGTACT AGT^ACT StuI 391 AGGCCT AGG^CCT Tth111I 1222 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1412 CCAACGGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1578 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 398 GGTACC G^GTACC 1856 GGTACC AccI 1372 GTCTAC GT^[AC][GT]AC 1552 GTAGAC Aor51HI 611 AGCGCT AGC^GCT 2180 AGCGCT ApaI 349 GGGCCC GGGCC^C 361 GGGCCC Asp718I 398 GGTACC G^GTACC 1856 GGTACC BalI 191 TGGCCA TGG^CCA 582 TGGCCA BbvCI 159 GCTGAGG CCTCAGC(-5/-2) 246 GCTGAGG BstYI 1787 AGATCC [GA]^GATC[CT] 2186 GGATCC DraIII 1534 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1639 CACCGCGTG Eco47III 611 AGCGCT AGC^GCT 2180 AGCGCT HgaI 1668 GACGC GACGC(5/10) 1139 GCGTC HinfI 72 GACTC G^A[ACGT]TC 1885 GAGTC KpnI 398 GGTACC GGTAC^C 1856 GGTACC MflI 1787 AGATCC [GA]^GATC[CT] 2186 GGATCC MluNI 191 TGGCCA TGG^CCA 582 TGGCCA MscI 191 TGGCCA TGG^CCA 582 TGGCCA MseI 1292 TTAA T^TAA 1304 TTAA MspA1I 796 CCGCTG C[AC]G^C[GT]G 861 CCGCGG PleI 72 GACTC GAGTC(4/5) 1885 GAGTC SbfI 462 CCTGCAGG CCTGCA^GG 1090 CCTGCAGG SexAI 1805 ACCAGGT A^CC[AT]GGT 1991 ACCTGGT SmaI 66 CCCGGG CCC^GGG 566 CCCGGG SphI 1097 GCATGC GCATG^C 1437 GCATGC Sse8387I 462 CCTGCAGG CCTGCA^GG 1090 CCTGCAGG Tru9I 1292 TTAA T^TAA 1304 TTAA Tsp509I 1294 AATT ^AATT 1378 AATT XhoII 1787 AGATCC [GA]^GATC[CT] 2186 GGATCC XmaCI 66 CCCGGG C^CCGGG 566 CCCGGG XmaI 66 CCCGGG C^CCGGG 566 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AfaI AluI Alw44I AlwI AlwNI ApaLI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I