(Circular) (Six-base) MAPSORT of: pFN21AB5762.seq Check: 5869 from: 1 to: 6958 pFN21AB5762 6958 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2698 3103 2698 Size: 405 6553 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4768 278 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3378 3558 4301 1937 Size: 1441 180 743 4594 Fragments arranged by size: 4594 1441 743 180 Acc65I G'GTAC_C Cuts at: 2403 3861 4279 2403 Size: 1458 418 5082 Fragments arranged by size: 5082 1458 418 AclI AA'CG_TT Cuts at: 1563 5085 5458 1563 Size: 3522 373 3063 Fragments arranged by size: 3522 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2917 3359 5021 6405 6673 1632 Size: 515 770 442 1662 1384 268 1917 Fragments arranged by size: 1917 1662 1384 770 515 442 268 AfeI AGC'GCT Cuts at: 2618 4187 2618 Size: 1569 5389 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6761 AflIII A'CryG_T Cuts at: 1203 5857 6834 1203 Size: 4654 977 1327 Fragments arranged by size: 4654 1327 977 AgeI A'CCGG_T Cuts at: 5789 5789 Size: 6958 AhdI GACnn_n'nnGTC Cuts at: 5687 5687 Size: 6958 AleI CACnn'nnGTG Cuts at: 1166 2561 3275 1166 Size: 1395 714 4849 Fragments arranged by size: 4849 1395 714 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2969 3521 1184 Size: 1785 552 4621 Fragments arranged by size: 4621 1785 552 AlwNI CAG_nnn'CTG Cuts at: 2687 3013 3352 3598 3817 4209 4701 6273 2687 Size: 326 339 246 219 392 492 1572 3372 Fragments arranged by size: 3372 1572 492 392 339 326 246 219 ApaI G_GGCC'C Cuts at: 2358 2370 2358 Size: 12 6946 ApaLI G'TGCA_C Cuts at: 2135 3020 3545 5015 6171 2135 Size: 885 525 1470 1156 2922 Fragments arranged by size: 2922 1470 1156 885 525 ApoI r'AATT_y Cuts at: 1493 4267 4493 4645 6754 1493 Size: 2774 226 152 2109 1697 Fragments arranged by size: 2774 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5512 160 Size: 872 4480 1606 Fragments arranged by size: 4480 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2571 3243 3479 3637 4158 4283 Size: 73 128 500 672 236 158 521 125 Cuts at: 4283 1870 Size: 4545 Fragments arranged by size: 4545 672 521 500 236 158 128 125 73 BamHI G'GATC_C Cuts at: 4191 4288 4655 4191 Size: 97 367 6494 Fragments arranged by size: 6494 367 97 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2403 3277 3575 3861 4279 Size: 325 217 627 616 874 298 286 418 Cuts at: 4279 5734 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 874 627 616 418 325 298 286 217 BanII G_rGCy'C Cuts at: 1391 2067 2358 2370 2661 2741 3250 3638 4277 Size: 676 291 12 291 80 509 388 639 Cuts at: 4277 1391 Size: 4072 Fragments arranged by size: 4072 676 639 509 388 291 291 80 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5987 BbvCI CC'TCA_GC Cuts at: 2165 2252 2165 Size: 87 6871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3234 3268 5149 5183 1903 Size: 34 50 34 1213 34 1881 34 3678 Fragments arranged by size: 3678 1881 1213 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4850 6060 4850 Size: 1210 5748 BclI T'GATC_A Cuts at: 1548 1548 Size: 6958 BfrBI ATG'CAT Cuts at: 3442 3442 Size: 6958 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3286 5569 Size: 107 122 71 631 605 337 1277 2283 Cuts at: 5569 136 Size: 1525 Fragments arranged by size: 2283 1525 1277 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6953 6953 Size: 6958 BlpI GC'TnA_GC Cuts at: 4375 4375 Size: 6958 Bme1580I G_kGCm'C Cuts at: 1790 2139 2358 2370 2694 3024 3282 3549 5019 Size: 349 219 12 324 330 258 267 1470 Cuts at: 5019 6175 1790 Size: 1156 2573 Fragments arranged by size: 2573 1470 1156 349 330 324 267 258 219 12 BmgBI CAC'GTC Cuts at: 1318 2782 1318 Size: 1464 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2658 2704 4689 Size: 416 53 83 399 24 1241 46 1985 Cuts at: 4689 5647 442 Size: 958 1753 Fragments arranged by size: 1985 1753 1241 958 416 399 83 53 46 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6958 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4138 4138 Size: 6958 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2164 2675 2813 2960 2989 3652 3686 5618 Size: 925 511 138 147 29 663 34 1932 Cuts at: 5618 1239 Size: 2579 Fragments arranged by size: 2579 1932 925 663 511 147 138 34 29 Bpu10I CC'TnA_GC Cuts at: 2111 2165 2252 2111 Size: 54 87 6817 Fragments arranged by size: 6817 87 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5083 5948 6246 5083 Size: 865 298 5795 Fragments arranged by size: 5795 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3176 5621 882 Size: 617 1677 2445 2219 Fragments arranged by size: 2445 2219 1677 617 BsaAI yAC'GTr Cuts at: 493 1204 4749 493 Size: 711 3545 2702 Fragments arranged by size: 3545 2702 711 BsaBI GATnn'nnATC Cuts at: 6952 6952 Size: 6958 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4765 5147 5782 275 Size: 53 83 186 936 3232 382 635 1451 Fragments arranged by size: 3232 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5391 5789 6063 6210 6621 5391 Size: 398 274 147 411 5728 Fragments arranged by size: 5728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 2696 2726 1178 Size: 30 165 30 993 30 270 30 5410 Fragments arranged by size: 5410 993 270 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2651 2842 2849 2983 2986 3851 1186 Size: 1258 207 191 7 134 3 865 4293 Fragments arranged by size: 4293 1258 865 207 191 134 7 3 BseYI C'CCAG_C Cuts at: 2019 2687 2879 3129 6161 6657 2019 Size: 668 192 250 3032 496 2320 Fragments arranged by size: 3032 2320 668 496 250 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 3491 3922 4010 1163 Size: 573 888 867 431 88 4111 Fragments arranged by size: 4111 888 867 573 431 88 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4408 5169 6197 1540 Size: 275 186 2407 761 1028 2301 Fragments arranged by size: 2407 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2386 2661 2681 2958 3024 3549 5019 5104 Size: 247 275 20 277 66 525 1470 85 Cuts at: 5104 6175 2139 Size: 1071 2922 Fragments arranged by size: 2922 1470 1071 525 277 275 247 85 66 20 BsmI GAATG_Cn' Cuts at: 4469 4562 4469 Size: 93 6865 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2139 2358 2370 2386 2661 2681 Size: 399 277 72 219 12 16 275 20 Cuts at: 2681 2694 2741 2958 3024 3250 3282 3549 3638 Size: 13 47 217 66 226 32 267 89 Cuts at: 3638 4277 5019 5104 6175 1391 Size: 639 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 399 277 275 267 226 219 217 89 85 72 66 47 32 20 16 13 12 BspEI T'CCGG_A Cuts at: 6621 6621 Size: 6958 BspHI T'CATG_A Cuts at: 3151 4847 6577 3151 Size: 1696 1730 3532 Fragments arranged by size: 3532 1730 1696 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2698 2998 3103 3741 4313 844 Size: 1854 300 105 638 572 3489 Fragments arranged by size: 3489 1854 638 572 300 105 BsrBI CCG'CTC Cuts at: 1394 1823 2898 4845 1394 Size: 429 1075 1947 3507 Fragments arranged by size: 3507 1947 1075 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3039 5453 5627 65 Size: 1183 1791 2414 174 1396 Fragments arranged by size: 2414 1791 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 5602 5789 1700 Size: 110 198 3594 187 2869 Fragments arranged by size: 3594 2869 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6958 BssSI C'ACGA_G Cuts at: 2663 5018 6030 2663 Size: 2355 1012 3591 Fragments arranged by size: 3591 2355 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2284 2447 3174 794 Size: 1490 163 727 4578 Fragments arranged by size: 4578 1490 727 163 BstBI TT'CG_AA Cuts at: 5762 5762 Size: 6958 BstEII G'GTnAC_C Cuts at: 1184 2315 1184 Size: 1131 5827 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2653 3196 3521 2194 Size: 459 543 325 5631 Fragments arranged by size: 5631 543 459 325 BstYI r'GATC_y Cuts at: 1972 3792 4191 4288 4655 5040 5057 6509 6595 Size: 1820 399 97 367 385 17 1452 86 Cuts at: 6595 6607 6953 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1820 1452 399 385 367 346 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4197 4197 Size: 6958 BtgI C'CryG_G Cuts at: 513 1065 2025 2055 2193 2700 2866 2920 3493 Size: 552 960 30 138 507 166 54 573 Cuts at: 3493 3801 3945 4077 513 Size: 308 144 132 3394 Fragments arranged by size: 3394 960 573 552 507 308 166 144 138 132 54 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6219 Fragments arranged by size: 6219 683 56 BtsI GCAGTG_nn' Cuts at: 2139 3075 3596 3879 4474 5268 5288 2139 Size: 936 521 283 595 794 20 3809 Fragments arranged by size: 3809 936 794 595 521 283 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6958 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6923 DraI TTT'AAA Cuts at: 3310 4262 3310 Size: 952 6006 DraIII CAC_nnn'GTG Cuts at: 3544 3649 3544 Size: 105 6853 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2786 5965 809 Size: 507 1470 3179 1802 Fragments arranged by size: 3179 1802 1470 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2196 2587 Size: 54 1425 50 275 18 222 144 391 Cuts at: 2587 3804 4094 4405 5294 6923 8 Size: 1217 290 311 889 1629 43 Fragments arranged by size: 1629 1425 1217 889 391 311 290 275 222 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4405 1537 Size: 275 2593 4090 Fragments arranged by size: 4090 2593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2852 4888 1353 Size: 1499 2036 3423 Fragments arranged by size: 3423 2036 1499 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 3045 5545 5919 6065 387 Size: 1064 138 486 970 2500 374 146 1280 Fragments arranged by size: 2500 1280 1064 970 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2659 2659 Size: 6958 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2164 2675 2813 2917 2960 2989 Size: 393 515 17 511 138 104 43 29 Cuts at: 2989 3359 3652 3686 5021 5618 6405 6673 1239 Size: 370 293 34 1335 597 787 268 1524 Fragments arranged by size: 1524 1335 787 597 515 511 393 370 293 268 138 104 43 34 29 17 EcoNI CCTnn'n_nnAGG Cuts at: 3856 3856 Size: 6958 EcoO109I rG'GnC_Cy Cuts at: 2354 2355 2366 2367 2797 3010 3133 3937 3994 Size: 1 11 1 430 213 123 804 57 Cuts at: 3994 4117 4242 2354 Size: 123 125 5070 Fragments arranged by size: 5070 804 430 213 125 123 123 57 11 1 1 EcoRI G'AATT_C Cuts at: 4267 4645 4267 Size: 378 6580 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3604 3604 Size: 6958 FspI TGC'GCA Cuts at: 5464 5464 Size: 6958 HaeII r_GCGC'y Cuts at: 1678 2596 2620 3077 3694 4189 6105 1678 Size: 918 24 457 617 495 1916 2531 Fragments arranged by size: 2531 1916 918 617 495 457 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5599 5631 5673 5705 1373 Size: 32 135 32 4027 32 42 32 2626 Fragments arranged by size: 4027 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4302 4548 669 Size: 988 281 2364 246 3079 Fragments arranged by size: 3079 2364 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6958 HpaI GTT'AAC Cuts at: 4548 4548 Size: 6958 KpnI G_GTAC'C Cuts at: 2407 3865 4283 2407 Size: 1458 418 5082 Fragments arranged by size: 5082 1458 418 MfeI C'AATT_G Cuts at: 4557 4557 Size: 6958 MluI A'CGCG_T Cuts at: 6834 6834 Size: 6958 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3327 6072 6256 1305 Size: 2022 2745 184 2007 Fragments arranged by size: 2745 2022 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 2589 10 Size: 54 1425 343 366 391 4379 Fragments arranged by size: 4379 1425 391 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2192 2222 2331 2561 2891 3213 Size: 648 873 153 30 109 230 330 322 Cuts at: 3213 3275 4916 5275 5434 518 Size: 62 1641 359 159 2042 Fragments arranged by size: 2042 1641 873 648 359 330 322 230 159 153 109 62 30 MspA1I CmG'CkG Cuts at: 1606 2803 2868 4374 5051 6199 6444 1606 Size: 1197 65 1506 677 1148 245 2120 Fragments arranged by size: 2120 1506 1197 1148 677 245 65 NaeI GCC'GGC Cuts at: 2010 2010 Size: 6958 NcoI C'CATG_G Cuts at: 513 1065 2055 2193 3801 513 Size: 552 990 138 1608 3670 Fragments arranged by size: 3670 1608 990 552 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 6958 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 6958 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6958 NotI GC'GGCC_GC Cuts at: 4405 4405 Size: 6958 NsiI A_TGCA'T Cuts at: 3444 3444 Size: 6958 NspI r_CATG'y Cuts at: 1132 1229 2380 3106 3446 4316 5861 1132 Size: 97 1151 726 340 870 1545 2229 Fragments arranged by size: 2229 1545 1151 870 726 340 97 PasI CC'CwG_GG Cuts at: 1348 2014 3762 3933 1348 Size: 666 1748 171 4373 Fragments arranged by size: 4373 1748 666 171 PciI A'CATG_T Cuts at: 5857 5857 Size: 6958 PflMI CCAn_nnn'nTGG Cuts at: 1108 3352 3630 3802 3852 1108 Size: 2244 278 172 50 4214 Fragments arranged by size: 4214 2244 278 172 50 PfoI T'CCnGG_A Cuts at: 2790 3195 2790 Size: 405 6553 PmeI GTTT'AAAC Cuts at: 4262 4262 Size: 6958 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5027 5059 6540 6572 5027 Size: 32 1481 32 5413 Fragments arranged by size: 5413 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2797 3010 3937 3994 4117 4242 2797 Size: 213 927 57 123 125 5513 Fragments arranged by size: 5513 927 213 125 123 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6958 PsiI TTA'TAA Cuts at: 4528 4528 Size: 6958 PspOMI G'GGCC_C Cuts at: 2354 2366 2354 Size: 12 6946 PstI C_TGCA'G Cuts at: 830 2049 2175 2472 3009 3100 3603 3792 4310 Size: 1219 126 297 537 91 503 189 518 Cuts at: 4310 830 Size: 3478 Fragments arranged by size: 3478 1219 537 518 503 297 189 126 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6958 SacI G_AGCT'C Cuts at: 2661 2661 Size: 6958 SacII CC_GC'GG Cuts at: 2869 2869 Size: 6958 SalI G'TCGA_C Cuts at: 1936 4300 1936 Size: 2364 4594 SanDI GG'GwC_CC Cuts at: 3937 3937 Size: 6958 SbfI CC_TGCA'GG Cuts at: 2472 3100 4310 2472 Size: 628 1210 5120 Fragments arranged by size: 5120 1210 628 ScaI AGT'ACT Cuts at: 3680 5206 6918 3680 Size: 1526 1712 3720 Fragments arranged by size: 3720 1712 1526 SexAI A'CCwGG_T Cuts at: 3810 3996 3810 Size: 186 6772 SfcI C'TryA_G Cuts at: 826 1045 2045 2171 2468 3005 3096 3599 3788 Size: 219 1000 126 297 537 91 503 189 Cuts at: 3788 4246 4306 5441 6122 6313 826 Size: 458 60 1135 681 191 1471 Fragments arranged by size: 1471 1135 1000 681 537 503 458 297 219 191 189 126 91 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6958 SmaI CCC'GGG Cuts at: 1872 2073 2573 4285 1872 Size: 201 500 1712 4545 Fragments arranged by size: 4545 1712 500 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 5062 5963 6225 820 Size: 197 926 3119 901 262 1553 Fragments arranged by size: 3119 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4749 493 Size: 4256 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6958 SphI G_CATG'C Cuts at: 1132 3106 3446 4316 1132 Size: 1974 340 870 3774 Fragments arranged by size: 3774 1974 870 340 SspI AAT'ATT Cuts at: 5 52 4882 5 Size: 47 4830 2081 Fragments arranged by size: 4830 2081 47 StuI AGG'CCT Cuts at: 1517 2398 1517 Size: 881 6077 StyI C'CwwG_G Cuts at: 513 1065 2055 2193 3801 4397 513 Size: 552 990 138 1608 596 3074 Fragments arranged by size: 3074 1608 990 596 552 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3527 3997 4985 5002 5155 5340 5785 Size: 1255 1947 470 988 17 153 185 445 Cuts at: 5785 325 Size: 1498 Fragments arranged by size: 1947 1498 1255 988 470 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3678 5204 6916 Size: 274 80 33 51 1443 1701 1526 1712 Cuts at: 6916 96 Size: 138 Fragments arranged by size: 1712 1701 1526 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 3284 3316 6918 6950 2696 Size: 32 556 32 3602 32 2704 Fragments arranged by size: 3602 2704 556 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3230 1316 Size: 1914 5044 XbaI T'CTAG_A Cuts at: 4294 4294 Size: 6958 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3424 2011 Size: 1413 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6958 XmaI C'CCGG_G Cuts at: 1870 2071 2571 4283 1870 Size: 201 500 1712 4545 Fragments arranged by size: 4545 1712 500 201 XmnI GAAnn'nnTTC Cuts at: 3587 5087 3587 Size: 1500 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4766 276 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI SwaI