(Linear) (Six-base) MAPSORT of: KIBB5762.seq Check: 4511 from: 1 to: 2253 KIBB5762.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 693 1098 2253 Size: 693 405 1155 Fragments arranged by size: 1155 693 405 AccI GT'mk_AC Cuts at: 0 1373 1553 2253 Size: 1373 180 700 Fragments arranged by size: 1373 700 180 Acc65I G'GTAC_C Cuts at: 0 398 1856 2253 Size: 398 1458 397 Fragments arranged by size: 1458 398 397 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 912 1354 2253 Size: 142 770 442 899 Fragments arranged by size: 899 770 442 142 AfeI AGC'GCT Cuts at: 0 613 2182 2253 Size: 613 1569 71 Fragments arranged by size: 1569 613 71 AleI CACnn'nnGTG Cuts at: 0 556 1270 2253 Size: 556 714 983 Fragments arranged by size: 983 714 556 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 964 1516 2253 Size: 964 552 737 Fragments arranged by size: 964 737 552 AlwNI CAG_nnn'CTG Cuts at: 0 682 1008 1347 1593 1812 2204 2253 Size: 682 326 339 246 219 392 49 Fragments arranged by size: 682 392 339 326 246 219 49 ApaI G_GGCC'C Cuts at: 0 353 365 2253 Size: 353 12 1888 Fragments arranged by size: 1888 353 12 ApaLI G'TGCA_C Cuts at: 0 130 1015 1540 2253 Size: 130 885 525 713 Fragments arranged by size: 885 713 525 130 AvaI C'yCGr_G Cuts at: 0 66 566 1238 1474 1632 2153 2253 Size: 66 500 672 236 158 521 100 Fragments arranged by size: 672 521 500 236 158 100 66 BamHI G'GATC_C Cuts at: 0 2186 2253 Size: 2186 67 BanI G'GyrC_C Cuts at: 0 398 1272 1570 1856 2253 Size: 398 874 298 286 397 Fragments arranged by size: 874 398 397 298 286 BanII G_rGCy'C Cuts at: 0 62 353 365 656 736 1245 1633 2253 Size: 62 291 12 291 80 509 388 620 Fragments arranged by size: 620 509 388 291 291 80 62 12 BbvCI CC'TCA_GC Cuts at: 0 160 247 2253 Size: 160 87 2006 Fragments arranged by size: 2006 160 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1229 1263 2253 Size: 1229 34 990 Fragments arranged by size: 1229 990 34 BfrBI ATG'CAT Cuts at: 0 1437 2253 Size: 1437 816 BglI GCCn_nnn'nGGC Cuts at: 0 1281 2253 Size: 1281 972 Bme1580I G_kGCm'C Cuts at: 0 134 353 365 689 1019 1277 1544 2253 Size: 134 219 12 324 330 258 267 709 Fragments arranged by size: 709 330 324 267 258 219 134 12 BmgBI CAC'GTC Cuts at: 0 777 2253 Size: 777 1476 BmrI ACTGGGnnnn_n' Cuts at: 0 653 699 2253 Size: 653 46 1554 Fragments arranged by size: 1554 653 46 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2133 2253 Size: 2133 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 670 808 955 984 1647 1681 2253 Size: 159 511 138 147 29 663 34 572 Fragments arranged by size: 663 572 511 159 147 138 34 29 Bpu10I CC'TnA_GC Cuts at: 0 106 160 247 2253 Size: 106 54 87 2006 Fragments arranged by size: 2006 106 87 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 1171 2253 Size: 1171 1082 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 391 421 691 721 2253 Size: 391 30 270 30 1532 Fragments arranged by size: 1532 391 270 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 439 646 837 844 978 981 1846 2253 Size: 439 207 191 7 134 3 865 407 Fragments arranged by size: 865 439 407 207 191 134 7 3 BseYI C'CCAG_C Cuts at: 0 14 682 874 1124 2253 Size: 14 668 192 250 1129 Fragments arranged by size: 1129 668 250 192 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 1486 1917 2005 2253 Size: 619 867 431 88 248 Fragments arranged by size: 867 619 431 248 88 BsiHKAI G_wGCw'C Cuts at: 0 134 381 656 676 953 1019 1544 2253 Size: 134 247 275 20 277 66 525 709 Fragments arranged by size: 709 525 277 275 247 134 66 20 Bsp1286I G_dGCh'C Cuts at: 0 62 134 353 365 381 656 676 689 Size: 62 72 219 12 16 275 20 13 Cuts at: 689 736 953 1019 1245 1277 1544 1633 2253 Size: 47 217 66 226 32 267 89 620 Fragments arranged by size: 620 275 267 226 219 217 89 72 66 62 47 32 20 16 13 12 BspHI T'CATG_A Cuts at: 0 1146 2253 Size: 1146 1107 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 693 993 1098 1736 2253 Size: 693 300 105 638 517 Fragments arranged by size: 693 638 517 300 105 BsrBI CCG'CTC Cuts at: 0 893 2253 Size: 893 1360 BsrDI GCAATG_nn' Cuts at: 0 1034 2253 Size: 1034 1219 BsrFI r'CCGG_y Cuts at: 0 3 2253 Size: 3 2250 BssSI C'ACGA_G Cuts at: 0 658 2253 Size: 658 1595 BstAPI GCAn_nnn'nTGC Cuts at: 0 279 442 1169 2253 Size: 279 163 727 1084 Fragments arranged by size: 1084 727 279 163 BstEII G'GTnAC_C Cuts at: 0 310 2253 Size: 310 1943 BstXI CCAn_nnnn'nTGG Cuts at: 0 189 648 1191 1516 2253 Size: 189 459 543 325 737 Fragments arranged by size: 737 543 459 325 189 BstYI r'GATC_y Cuts at: 0 1787 2186 2253 Size: 1787 399 67 Fragments arranged by size: 1787 399 67 Bsu36I CC'TnA_GG Cuts at: 0 2192 2253 Size: 2192 61 BtgI C'CryG_G Cuts at: 0 20 50 188 695 861 915 1488 1796 Size: 20 30 138 507 166 54 573 308 Cuts at: 1796 1940 2072 2253 Size: 144 132 181 Fragments arranged by size: 573 507 308 181 166 144 138 132 54 30 20 BtsI GCAGTG_nn' Cuts at: 0 134 1070 1591 1874 2253 Size: 134 936 521 283 379 Fragments arranged by size: 936 521 379 283 134 DraI TTT'AAA Cuts at: 0 1305 2253 Size: 1305 948 DraIII CAC_nnn'GTG Cuts at: 0 1539 1644 2253 Size: 1539 105 609 Fragments arranged by size: 1539 609 105 DrdI GACnn_nn'nnGTC Cuts at: 0 781 2253 Size: 781 1472 EaeI y'GGCC_r Cuts at: 0 47 191 582 1799 2089 2253 Size: 47 144 391 1217 290 164 Fragments arranged by size: 1217 391 290 164 144 47 EarI CTCTTCn'nnn_ Cuts at: 0 847 2253 Size: 847 1406 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 70 1040 2253 Size: 70 970 1213 Fragments arranged by size: 1213 970 70 EcoICRI GAG'CTC Cuts at: 0 654 2253 Size: 654 1599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 159 670 808 912 955 984 1354 Size: 142 17 511 138 104 43 29 370 Cuts at: 1354 1647 1681 2253 Size: 293 34 572 Fragments arranged by size: 572 511 370 293 142 138 104 43 34 29 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 1851 2253 Size: 1851 402 EcoO109I rG'GnC_Cy Cuts at: 0 349 350 361 362 792 1005 1128 1932 Size: 349 1 11 1 430 213 123 804 Cuts at: 1932 1989 2112 2237 2253 Size: 57 123 125 16 Fragments arranged by size: 804 430 349 213 125 123 123 57 16 11 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1599 2253 Size: 1599 654 HaeII r_GCGC'y Cuts at: 0 591 615 1072 1689 2184 2253 Size: 591 24 457 617 495 69 Fragments arranged by size: 617 591 495 457 69 24 KpnI G_GTAC'C Cuts at: 0 402 1860 2253 Size: 402 1458 393 Fragments arranged by size: 1458 402 393 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1322 2253 Size: 1322 931 MscI TGG'CCA Cuts at: 0 193 584 2253 Size: 193 391 1669 Fragments arranged by size: 1669 391 193 MslI CAynn'nnrTG Cuts at: 0 34 187 217 326 556 886 1208 1270 Size: 34 153 30 109 230 330 322 62 Cuts at: 1270 2253 Size: 983 Fragments arranged by size: 983 330 322 230 153 109 62 34 30 MspA1I CmG'CkG Cuts at: 0 798 863 2253 Size: 798 65 1390 Fragments arranged by size: 1390 798 65 NaeI GCC'GGC Cuts at: 0 5 2253 Size: 5 2248 NcoI C'CATG_G Cuts at: 0 50 188 1796 2253 Size: 50 138 1608 457 Fragments arranged by size: 1608 457 138 50 NgoMIV G'CCGG_C Cuts at: 0 3 2253 Size: 3 2250 NsiI A_TGCA'T Cuts at: 0 1439 2253 Size: 1439 814 NspI r_CATG'y Cuts at: 0 375 1101 1441 2253 Size: 375 726 340 812 Fragments arranged by size: 812 726 375 340 PasI CC'CwG_GG Cuts at: 0 9 1757 1928 2253 Size: 9 1748 171 325 Fragments arranged by size: 1748 325 171 9 PflMI CCAn_nnn'nTGG Cuts at: 0 1347 1625 1797 1847 2253 Size: 1347 278 172 50 406 Fragments arranged by size: 1347 406 278 172 50 PfoI T'CCnGG_A Cuts at: 0 785 1190 2253 Size: 785 405 1063 Fragments arranged by size: 1063 785 405 PpuMI rG'GwC_Cy Cuts at: 0 792 1005 1932 1989 2112 2237 2253 Size: 792 213 927 57 123 125 16 Fragments arranged by size: 927 792 213 125 123 57 16 PspOMI G'GGCC_C Cuts at: 0 349 361 2253 Size: 349 12 1892 Fragments arranged by size: 1892 349 12 PstI C_TGCA'G Cuts at: 0 44 170 467 1004 1095 1598 1787 2253 Size: 44 126 297 537 91 503 189 466 Fragments arranged by size: 537 503 466 297 189 126 91 44 SacI G_AGCT'C Cuts at: 0 656 2253 Size: 656 1597 SacII CC_GC'GG Cuts at: 0 864 2253 Size: 864 1389 SanDI GG'GwC_CC Cuts at: 0 1932 2253 Size: 1932 321 SbfI CC_TGCA'GG Cuts at: 0 467 1095 2253 Size: 467 628 1158 Fragments arranged by size: 1158 628 467 ScaI AGT'ACT Cuts at: 0 1675 2253 Size: 1675 578 SexAI A'CCwGG_T Cuts at: 0 1805 1991 2253 Size: 1805 186 262 Fragments arranged by size: 1805 262 186 SfcI C'TryA_G Cuts at: 0 40 166 463 1000 1091 1594 1783 2241 Size: 40 126 297 537 91 503 189 458 Cuts at: 2241 2253 Size: 12 Fragments arranged by size: 537 503 458 297 189 126 91 40 12 SmaI CCC'GGG Cuts at: 0 68 568 2253 Size: 68 500 1685 Fragments arranged by size: 1685 500 68 SphI G_CATG'C Cuts at: 0 1101 1441 2253 Size: 1101 340 812 Fragments arranged by size: 1101 812 340 StuI AGG'CCT Cuts at: 0 393 2253 Size: 393 1860 StyI C'CwwG_G Cuts at: 0 50 188 1796 2253 Size: 50 138 1608 457 Fragments arranged by size: 1608 457 138 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1522 1992 2253 Size: 1522 470 261 Fragments arranged by size: 1522 470 261 TatI w'GTAC_w Cuts at: 0 1673 2253 Size: 1673 580 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 691 723 1279 1311 2253 Size: 691 32 556 32 942 Fragments arranged by size: 942 691 556 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1225 2253 Size: 1225 1028 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1419 2253 Size: 1419 834 XmaI C'CCGG_G Cuts at: 0 66 566 2253 Size: 66 500 1687 Fragments arranged by size: 1687 500 66 XmnI GAAnn'nnTTC Cuts at: 0 1582 2253 Size: 1582 671 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbvCI BcgI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI SacI SacII SanDI SbfI ScaI SexAI SfcI SmaI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI BclI BglII BlpI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI EagI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SapI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI