Restriction map for
pFN21AE6757
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BfaI BfrI
BglII BlnI BsaAI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
Eam1105I Eco47III Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1131 GGTACC G^GTACC
AccI 790 GTCGAC GT^[AC][GT]AC
AccII 863 CGCG CG^CG
AcsI 22 GAATTC [GA]^AATT[CT]
AcyI 787 GGCGTC G[GA]^CG[CT]C
ApoI 22 GAATTC [GA]^AATT[CT]
Asp700I 630 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1131 GGTACC G^GTACC
AspI 370 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1327 GGATCC G^GATCC
BanI 1131 GGTACC G^G[CT][GA]CC
BlpI 1411 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1411 GCTGAGC GC^T[ACGT]AGC
BsaHI 787 GGCGTC G[GA]^CG[CT]C
BsaI 131 GAGACC GGTCTC(1/5)
BsgI 277 CTGCAC GTGCAG(16/14)
BsiEI 187 CGGCCG CG[GA][CT]^CG
BspHI 833 TCATGA T^CATGA
BsrDI 1059 GCAATG GCAATG(2/0)
BssSI 124 CTCGTG CACGAG(-5/-1)
BstBI 461 TTCGAA TT^CGAA
BstUI 863 CGCG CG^CG
CelII 1411 GCTGAGC GC^T[ACGT]AGC
DraIII 208 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 107 CCATGG C^C[GA][CT]GG
EagI 187 CGGCCG C^GGCCG
EclXI 187 CGGCCG C^GGCCG
Eco52I 187 CGGCCG C^GGCCG
EcoRI 22 GAATTC G^AATTC
HaeII 239 GGCGCT [GA]GCGC^[CT]
Hin1I 787 GGCGTC G[GA]^CG[CT]C
HincII 790 GTCGAC GT[CT]^[GA]AC
HindII 790 GTCGAC GT[CT]^[GA]AC
HphI 1271 TCACC GGTGA(8/7)
KpnI 1131 GGTACC GGTAC^C
MaeII 113 ACGT A^CGT
MaeIII 1471 GTGAC ^GT[ACGT]AC
MseI 1052 TTAA T^TAA
MvnI 863 CGCG CG^CG
NcoI 107 CCATGG C^CATGG
NspV 461 TTCGAA TT^CGAA
PaeR7I 1177 CTCGAG C^TCGAG
PflFI 370 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 102 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 226 CAGCTG CAG^CTG
RcaI 833 TCATGA T^CATGA
SacI 1174 GAGCTC GAGCT^C
SalI 790 GTCGAC G^TCGAC
SapI 333 GCTCTTC GCTCTTC(1/4)
SexAI 209 ACCAGGT A^CC[AT]GGT
SfuI 461 TTCGAA TT^CGAA
SmlI 1177 CTCGAG C^T[CT][GA]AG
Tru9I 1052 TTAA T^TAA
Tsp45I 1471 GTGAC ^GT[GC]AC
Tth111I 370 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 102 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1177 CTCGAG C^TCGAG
XmnI 630 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 404 TCCGGA T^CCGGA
1232 TCCGGA
AspHI 121 GTGCTC G[AT]GC[AT]^C
1174 GAGCTC
BbsI 1069 GAAGAC GAAGAC(2/6)
634 GTCTTC
BclI 222 TGATCA T^GATCA
1155 TGATCA
BglI 644 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
979 GCCCAGAAGGC
BpuAI 1069 GAAGAC GAAGAC(2/6)
634 GTCTTC
BseAI 404 TCCGGA T^CCGGA
1232 TCCGGA
BsiHKAI 121 GTGCTC G[AT]GC[AT]^C
1174 GAGCTC
BsmAI 131 GAGAC GTCTC(1/5)
492 GAGAC
BspEI 404 TCCGGA T^CCGGA
1232 TCCGGA
BsrI 1073 ACTGG ACTGG(1/-1)
1535 ACTGG
BstXI 107 CCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
693 CCAGAAGCCTGG
BstYI 929 AGATCC [GA]^GATC[CT]
1327 GGATCC
CfoI 240 GCGC GCG^C
862 GCGC
DrdI 394 GACGAGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACCAGATTGTC
EaeI 116 TGGCCG [CT]^GGCC[GA]
187 CGGCCG
EarI 334 CTCTTC CTCTTC(1/4)
1042 GAAGAG
EcoNI 1485 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1506 CCTGCAGAAGG
EcoT14I 107 CCATGG C^C[AT][AT]GG
663 CCTTGG
FbaI 222 TGATCA T^GATCA
1155 TGATCA
HhaI 240 GCGC GCG^C
862 GCGC
HinP1I 240 GCGC G^CGC
862 GCGC
Ksp632I 334 CTCTTC CTCTTC(1/4)
1042 GAAGAG
MflI 929 AGATCC [GA]^GATC[CT]
1327 GGATCC
MroI 404 TCCGGA T^CCGGA
1232 TCCGGA
MslI 108 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
483 CATCAAAGTG
PleI 1360 GACTC GAGTC(4/5)
919 GAGTC
StyI 107 CCATGG C^C[AT][AT]GG
663 CCTTGG
TfiI 571 GAATC G^A[AT]TC
1521 GAATC
Tsp509I 23 AATT ^AATT
975 AATT
TspRI 200 CACTG CA[GC]TG[ACGT][ACGT]^
1113 CACTG
XcmI 717 CCATGTGAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1367 CCATTGGGAAGGTGG
XhoII 929 AGATCC [GA]^GATC[CT]
1327 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanII BbvCI BbvI BcnI
BmyI BpmI BsaJI BsaWI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TaqI TseI TthHB8I