Restriction map for
pFN21AE6757
(Circular) (Six-base) MAPSORT of: pFN21AE6757.seq Check: 1143 from: 1 to: 6304
pFN21AE6757 6304 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2796 3647 1937
Size: 859 851 4594
Fragments arranged by size:
4594 859 851
Acc65I G'GTAC_C
Cuts at: 3136 3625 3136
Size: 489 5815
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2228 2279 4367 5751 6019 1632
Size: 530 66 51 2088 1384 268 1917
Fragments arranged by size:
2088 1917 1384 530 268 66 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6304
AlwNI CAG_nnn'CTG
Cuts at: 4047 5619 4047
Size: 1572 4732
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 2027 3613 3839 3991 6100 1493
Size: 534 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 534 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2661 3182 3629 1870
Size: 73 79 639 521 447 4545
Fragments arranged by size:
4545 639 521 447 79 73
BamHI G'GATC_C
Cuts at: 3332 3634 4001 3332
Size: 302 367 5635
Fragments arranged by size:
5635 367 302
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3136 3625 5080 618
Size: 325 217 627 1349 489 1455 1842
Fragments arranged by size:
1842 1455 1349 627 489 325 217
BanII G_rGCy'C
Cuts at: 1391 2340 2388 2541 2606 3086 3183 3189 3623
Size: 949 48 153 65 480 97 6 434
Cuts at: 3623 1391
Size: 4072
Fragments arranged by size:
4072 949 480 434 153 97 65 48
6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2632 3081 928
Size: 971 733 449 4151
Fragments arranged by size:
4151 971 733 449
BbvCI CC'TCA_GC
Cuts at: 2748 3123 3495 2748
Size: 375 372 5557
Fragments arranged by size:
5557 375 372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 2227 3160 1548
Size: 679 933 4692
Fragments arranged by size:
4692 933 679
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2655 2990 4915
Size: 107 122 71 631 605 983 335 1925
Cuts at: 4915 136
Size: 1525
Fragments arranged by size:
1925 1525 983 631 605 335 122 107
71
BglII A'GATC_T
Cuts at: 6299 6299
Size: 6304
BlpI GC'TnA_GC
Cuts at: 3417 3721 3417
Size: 304 6000
Bme1580I G_kGCm'C
Cuts at: 1790 4365 5521 1790
Size: 2575 1156 2573
Fragments arranged by size:
2575 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2119 1318
Size: 801 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3088 4035 4993
Size: 416 53 83 399 24 1671 947 958
Cuts at: 4993 442
Size: 1753
Fragments arranged by size:
1753 1671 958 947 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2233 2342 2372 2924 4964 1239
Size: 994 109 30 552 2040 2579
Fragments arranged by size:
2579 2040 994 552 109 30
Bpu10I CC'TnA_GC
Cuts at: 2748 3123 3177 3495 2748
Size: 375 54 318 5557
Fragments arranged by size:
5557 375 318 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4429 5294 5592 4429
Size: 865 298 5141
Fragments arranged by size:
5141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2130 4967 882
Size: 617 631 2837 2219
Fragments arranged by size:
2837 2219 631 617
BsaAI yAC'GTr
Cuts at: 493 1204 4095 493
Size: 711 2891 2702
Fragments arranged by size:
2891 2702 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2793 4111 4493 5128
Size: 53 83 186 936 1260 1318 382 635
Cuts at: 5128 275
Size: 1451
Fragments arranged by size:
1451 1318 1260 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2409 3015 3237 4737 5135 5409 5556 5967 2409
Size: 606 222 1500 398 274 147 411 2746
Fragments arranged by size:
2746 1500 606 411 398 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6079
Fragments arranged by size:
6079 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2422 2462 3056 3230 1186
Size: 1236 40 594 174 4260
Fragments arranged by size:
4260 1236 594 174 40
BseYI C'CCAG_C
Cuts at: 2162 5507 6003 2162
Size: 3345 496 2463
Fragments arranged by size:
3345 2463 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 1163
Size: 573 531 5200
Fragments arranged by size:
5200 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2195 3754 4515 5543 1540
Size: 275 186 194 1559 761 1028 2301
Fragments arranged by size:
2301 1559 1028 761 275 194 186
BsiHKAI G_wGCw'C
Cuts at: 2130 3183 4365 4450 5521 2130
Size: 1053 1182 85 1071 2913
Fragments arranged by size:
2913 1182 1071 1053 85
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2340 2388 2541 2606 3086 3183
Size: 399 340 210 48 153 65 480 97
Cuts at: 3183 3189 3623 4365 4450 5521 1391
Size: 6 434 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 434 399 340 210
153 97 85 65 48 6
BspEI T'CCGG_A
Cuts at: 2409 3237 5967 2409
Size: 828 2730 2746
Fragments arranged by size:
2746 2730 828
BspHI T'CATG_A
Cuts at: 2838 4193 5923 2838
Size: 1355 1730 3219
Fragments arranged by size:
3219 1730 1355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3659 844
Size: 2815 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2854 2962 3406 3421 4191 1394
Size: 429 1031 108 444 15 770 3507
Fragments arranged by size:
3507 1031 770 444 429 108 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 3071 4799 4973 65
Size: 1183 1823 1728 174 1396
Fragments arranged by size:
1823 1728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 2129 4364 5376 2129
Size: 2235 1012 3057
Fragments arranged by size:
3057 2235 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6304
BstBI TT'CG_AA
Cuts at: 2467 5108 2467
Size: 2641 3663
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstXI CCAn_nnnn'nTGG
Cuts at: 2119 2705 2119
Size: 586 5718
BstYI r'GATC_y
Cuts at: 1972 2934 3332 3634 4001 4386 4403 5855 5941
Size: 962 398 302 367 385 17 1452 86
Cuts at: 5941 5953 6299 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 962 398 385 367 346 302
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 513
Size: 552 939 108 4705
Fragments arranged by size:
4705 939 552 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2933 487
Size: 683 56 1707 3858
Fragments arranged by size:
3858 1707 683 56
BtsI GCAGTG_nn'
Cuts at: 3117 3820 4614 4634 3117
Size: 703 794 20 4787
Fragments arranged by size:
4787 794 703 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 6304
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 2777 5311 809
Size: 507 1089 372 2534 1802
Fragments arranged by size:
2534 1802 1089 507 372
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2192 3751
Size: 54 1425 50 275 18 291 71 1559
Cuts at: 3751 4640 6269 8
Size: 889 1629 43
Fragments arranged by size:
1629 1559 1425 889 291 275 71 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2192 3751 1537
Size: 275 380 1559 4090
Fragments arranged by size:
4090 1559 380 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 3042 4234 1353
Size: 992 697 1192 3423
Fragments arranged by size:
3423 1192 992 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4891 5265 5411 387
Size: 1064 138 3302 374 146 1280
Fragments arranged by size:
3302 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3181 3181
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2228 2233 2279 2342 2372 2924
Size: 393 530 66 5 46 63 30 552
Cuts at: 2924 4367 4964 5751 6019 1239
Size: 1443 597 787 268 1524
Fragments arranged by size:
1524 1443 787 597 552 530 393 268
66 63 46 30 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3494 3515 3494
Size: 21 6283
EcoRI G'AATT_C
Cuts at: 2027 3613 3991 2027
Size: 1586 378 4340
Fragments arranged by size:
4340 1586 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 6304
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 2248 5451 1678
Size: 570 3203 2531
Fragments arranged by size:
3203 2531 570
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2412 2444 3068 3100 3251
Size: 32 135 32 840 32 624 32 151
Cuts at: 3251 3283 4945 4977 5019 5051 1373
Size: 32 1662 32 42 32 2626
Fragments arranged by size:
2626 1662 840 624 151 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 3648 3894 669
Size: 988 281 859 851 246 3079
Fragments arranged by size:
3079 988 859 851 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3140 3629 3140
Size: 489 5815
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 2952 5418 5602 1305
Size: 1248 399 2466 184 2007
Fragments arranged by size:
2466 2007 1248 399 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2492 4262 4621 4780 518
Size: 648 951 375 1770 359 159 2042
Fragments arranged by size:
2042 1770 951 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2233 2688 2887 3720 4397 5545 5790
Size: 591 36 455 199 833 677 1148 245
Cuts at: 5790 1606
Size: 2120
Fragments arranged by size:
2120 1148 833 677 591 455 245 199
36
NcoI C'CATG_G
Cuts at: 513 1065 2004 2112 513
Size: 552 939 108 4705
Fragments arranged by size:
4705 939 552 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 3662 5207 1132
Size: 97 2433 1545 2229
Fragments arranged by size:
2433 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6304
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 1108
Size: 1005 5299
PfoI T'CCnGG_A
Cuts at: 2249 2940 2249
Size: 691 5613
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3084 3116 3165 3197 4373 4405 5886 5918 3084
Size: 32 49 32 1176 32 1481 32 3470
Fragments arranged by size:
3470 1481 1176 49 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspXI vC'TCGA_Gb
Cuts at: 3182 3182
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2678 2886 3516 3656 830
Size: 1848 208 630 140 3478
Fragments arranged by size:
3478 1848 630 208 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2233 2233
Size: 6304
SacI G_AGCT'C
Cuts at: 3183 3183
Size: 6304
SalI G'TCGA_C
Cuts at: 1936 2795 3646 1936
Size: 859 851 4594
Fragments arranged by size:
4594 859 851
SapI GCTCTTCn'nnn_
Cuts at: 2345 2345
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 6304
SfcI C'TryA_G
Cuts at: 826 1045 2674 2882 3512 3652 4787 5468 5659
Size: 219 1629 208 630 140 1135 681 191
Cuts at: 5659 826
Size: 1471
Fragments arranged by size:
1629 1471 1135 681 630 219 208 191
140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3182 4408 5309 5571 820
Size: 197 926 1239 1226 901 262 1553
Fragments arranged by size:
1553 1239 1226 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2295 2667 3521 1517
Size: 778 372 854 4300
Fragments arranged by size:
4300 854 778 372
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2668 3743 513
Size: 552 939 108 556 1075 3074
Fragments arranged by size:
3074 1075 939 556 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2025 4331 4348 4501 4686 5131 325
Size: 1255 445 2306 17 153 185 445 1498
Fragments arranged by size:
2306 1498 1255 445 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4550 6262 96
Size: 274 80 33 51 1443 2573 1712 138
Fragments arranged by size:
2573 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6264 6296 6264
Size: 32 6272
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 5242
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 3379 2729
Size: 650 5654
XhoI C'TCGA_G
Cuts at: 1943 3182 1943
Size: 1239 5065
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 2639 4433 2639
Size: 1794 4510
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI