################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlnI BpuAI BsaAI BsaHI BseAI BsgI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MfeI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 635 GTAGAC GT^[AC][GT]AC AcsI 772 AAATTT [GA]^AATT[CT] AflIII 424 ACGCGT A^C[GA][CT]GT AocI 406 CCTAAGG CC^T[ACGT]AGG ApaI 117 GGGCCC GGGCC^C ApoI 772 AAATTT [GA]^AATT[CT] Asp700I 319 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 843 CTCGGG C^[CT]CG[GA]G BalI 306 TGGCCA TGG^CCA BamHI 1068 GGATCC G^GATCC BcgI 592 GCAACCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 666 TGATCA T^GATCA BpmI 694 CTCCAG CTGGAG(16/14) BsaBI 1224 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1283 GGTCTC GGTCTC(1/5) BsaWI 1287 TCCGGT [AT]^CCGG[AT] BsmBI 907 GAGACG CGTCTC(1/5) BsoBI 843 CTCGGG C^[CT]CG[GA]G Bsp1407I 1211 TGTACA T^GTACA BsrDI 273 GCAATG GCAATG(2/0) BsrFI 554 GCCGGC [GA]^CCGG[CT] BsrGI 1211 TGTACA T^GTACA BssSI 1119 CTCGTG CACGAG(-5/-1) BstYI 1068 GGATCC [GA]^GATC[CT] Bsu36I 406 CCTAAGG CC^T[ACGT]AGG Cfr10I 554 GCCGGC [GA]^CCGG[CT] DrdI 856 GACAGTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 306 TGGCCA [CT]^GGCC[GA] EarI 1195 GAAGAG CTCTTC(1/4) Eco81I 406 CCTAAGG CC^T[ACGT]AGG EcoT22I 1206 ATGCAT ATGCA^T FauI 146 GCGGG CCCGC(4/6) FbaI 666 TGATCA T^GATCA HaeII 173 GGCGCT [GA]GCGC^[CT] HindIII 1048 AAGCTT A^AGCTT Ksp632I 1195 GAAGAG CTCTTC(1/4) MamI 1224 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1068 GGATCC [GA]^GATC[CT] MluI 424 ACGCGT A^CGCGT MluNI 306 TGGCCA TGG^CCA MscI 306 TGGCCA TGG^CCA MseI 655 TTAA T^TAA NaeI 554 GCCGGC GCC^GGC NgoMIV 554 GCCGGC G^CCGGC NsiI 1206 ATGCAT ATGCA^T PflMI 1094 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 660 AGGTCCT [GA]G^G[AT]CC[CT] SacI 266 GAGCTC GAGCT^C SmlI 361 CTCAAG C^T[CT][GA]AG SspBI 1211 TGTACA T^GTACA Tru9I 655 TTAA T^TAA Van91I 1094 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 548 CCACCTGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1068 GGATCC [GA]^GATC[CT] XmnI 319 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 1069 GATCC GGATC(4/5) 1068 GGATC AspHI 266 GAGCTC G[AT]GC[AT]^C 1110 GTGCTC BcnI 93 CCGGG CC^[GC]GG 1143 CCGGG BfaI 400 CTAG C^TAG 755 CTAG BsiHKAI 266 GAGCTC G[AT]GC[AT]^C 1110 GTGCTC BsmFI 95 GGGAC GGGAC(10/14) 700 GGGAC BsmI 4 GAATGC GAATGC(1/-1) 130 GAATGC BsrI 309 CCAGT ACTGG(1/-1) 867 CCAGT BstXI 471 CCAAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1078 CCAGCCAGGTGG CfoI 174 GCGC GCG^C 1234 GCGC HhaI 174 GCGC GCG^C 1234 GCGC HinP1I 174 GCGC G^CGC 1234 GCGC HinfI 439 GAATC G^A[ACGT]TC 834 GATTC MaeI 400 CTAG C^TAG 755 CTAG NciI 93 CCGGG CC^[GC]GG 1143 CCGGG PvuII 493 CAGCTG CAG^CTG 952 CAGCTG TfiI 439 GAATC G^A[AT]TC 834 GATTC Tsp45I 1013 GTCAC ^GT[GC]AC 1183 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BanII BbvI BlpI BmyI Bpu1102I BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CelII Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp509I TspRI