(Circular) (Six-base) MAPSORT of: pFN21AE6374.seq Check: 1160 from: 1 to: 6022 pFN21AE6374 6022 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2156 2249 2564 2156 Size: 93 315 5614 Fragments arranged by size: 5614 315 93 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2641 3365 1937 Size: 704 724 4594 Fragments arranged by size: 4594 724 704 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3041 3177 4085 5469 5737 1632 Size: 1409 136 908 1384 268 1917 Fragments arranged by size: 1917 1409 1384 908 268 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 2429 4921 5898 1203 Size: 1226 2492 977 1327 Fragments arranged by size: 2492 1327 1226 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 2368 3194 3283 1166 Size: 1202 826 89 3905 Fragments arranged by size: 3905 1202 826 89 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AlwNI CAG_nnn'CTG Cuts at: 3765 5337 3765 Size: 1572 4450 ApaI G_GGCC'C Cuts at: 2126 2126 Size: 6022 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2777 3331 3557 3709 5818 1493 Size: 1284 554 226 152 2109 1697 Fragments arranged by size: 2109 1697 1284 554 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2848 3347 1870 Size: 73 905 499 4545 Fragments arranged by size: 4545 905 499 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2604 2637 2604 Size: 33 5989 BamHI G'GATC_C Cuts at: 3073 3352 3719 3073 Size: 279 367 5376 Fragments arranged by size: 5376 367 279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3343 4798 618 Size: 325 217 627 1556 1455 1842 Fragments arranged by size: 1842 1556 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2126 2275 2855 3341 1391 Size: 735 149 580 486 4072 Fragments arranged by size: 4072 735 580 486 149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5051 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2586 2620 4213 4247 1903 Size: 34 649 34 1593 34 3678 Fragments arranged by size: 3678 1593 649 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 2671 1548 Size: 1123 4899 BfrBI ATG'CAT Cuts at: 3213 3213 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 2274 2284 2932 3439 2274 Size: 10 648 507 4857 Fragments arranged by size: 4857 648 507 10 Bme1580I G_kGCm'C Cuts at: 1790 2126 4083 5239 1790 Size: 336 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 336 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3753 4711 442 Size: 416 53 83 399 24 2336 958 1753 Fragments arranged by size: 2336 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2684 4682 1239 Size: 1445 1998 2579 Fragments arranged by size: 2579 1998 1445 Bpu10I CC'TnA_GC Cuts at: 2186 2186 Size: 6022 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2351 4147 5012 5310 2351 Size: 1796 865 298 3063 Fragments arranged by size: 3063 1796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3294 4685 882 Size: 617 1795 1391 2219 Fragments arranged by size: 2219 1795 1391 617 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 3233 6016 3233 Size: 2783 3239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 4211 4846 275 Size: 53 83 186 936 2296 382 635 1451 Fragments arranged by size: 2296 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3292 4455 4853 5127 5274 5685 3292 Size: 1163 398 274 147 411 3629 Fragments arranged by size: 3629 1163 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2837 2867 2980 3010 1178 Size: 30 165 30 1434 30 113 30 4190 Fragments arranged by size: 4190 1434 165 113 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2105 2961 3001 3109 3112 3182 1186 Size: 847 72 856 40 108 3 70 4026 Fragments arranged by size: 4026 856 847 108 72 70 40 3 BseYI C'CCAG_C Cuts at: 2500 2586 5225 5721 2500 Size: 86 2639 496 2801 Fragments arranged by size: 2801 2639 496 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3472 4233 5261 1540 Size: 275 186 1471 761 1028 2301 Fragments arranged by size: 2301 1471 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2275 3119 4083 4168 5239 2275 Size: 844 964 85 1071 3058 Fragments arranged by size: 3058 1071 964 844 85 BsmI GAATG_Cn' Cuts at: 2015 2141 3533 3626 2015 Size: 126 1392 93 4411 Fragments arranged by size: 4411 1392 126 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2906 2906 Size: 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2275 2855 3119 3341 4083 4168 Size: 399 336 149 580 264 222 742 85 Cuts at: 4168 5239 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 580 399 336 264 222 149 85 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2156 2249 2564 3377 844 Size: 1312 93 315 813 3489 Fragments arranged by size: 3489 1312 813 315 93 BsrBI CCG'CTC Cuts at: 1394 1823 3909 1394 Size: 429 2086 3507 Fragments arranged by size: 3507 2086 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2285 4517 4691 65 Size: 1183 1037 2232 174 1396 Fragments arranged by size: 2232 1396 1183 1037 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2559 4666 4853 1700 Size: 110 749 2107 187 2869 Fragments arranged by size: 2869 2107 749 187 110 BsrGI T'GTAC_A Cuts at: 96 3216 96 Size: 3120 2902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 3124 4082 5094 3124 Size: 958 1012 4052 Fragments arranged by size: 4052 1012 958 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstXI CCAn_nnnn'nTGG Cuts at: 2483 3090 2483 Size: 607 5415 BstYI r'GATC_y Cuts at: 1972 3073 3352 3719 4104 4121 5573 5659 5671 Size: 1101 279 367 385 17 1452 86 12 Cuts at: 5671 6017 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1101 385 367 346 279 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2412 2412 Size: 6022 BtgI C'CryG_G Cuts at: 513 1065 2004 2126 2308 3104 513 Size: 552 939 122 182 796 3431 Fragments arranged by size: 3431 939 796 552 182 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3220 487 Size: 683 56 1994 3289 Fragments arranged by size: 3289 1994 683 56 BtsI GCAGTG_nn' Cuts at: 2036 3538 4332 4352 2036 Size: 1502 794 20 3706 Fragments arranged by size: 3706 1502 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2867 5029 809 Size: 507 1551 2162 1802 Fragments arranged by size: 2162 1802 1551 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2311 3469 4358 Size: 54 1425 50 275 18 481 1158 889 Cuts at: 4358 5987 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1158 889 481 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 1537 Size: 275 1657 4090 Fragments arranged by size: 4090 1657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3195 3952 1353 Size: 1842 757 3423 Fragments arranged by size: 3423 1842 757 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2273 2273 Size: 6022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2684 3041 3177 4085 4682 5469 5737 Size: 393 1052 357 136 908 597 787 268 Cuts at: 5737 1239 Size: 1524 Fragments arranged by size: 1524 1052 908 787 597 393 357 268 136 EcoO109I rG'GnC_Cy Cuts at: 2122 2160 2666 2122 Size: 38 506 5478 Fragments arranged by size: 5478 506 38 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 2182 5169 1678 Size: 504 2987 2531 Fragments arranged by size: 2987 2531 504 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4663 4695 4737 4769 1373 Size: 32 135 32 3091 32 42 32 2626 Fragments arranged by size: 3091 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 3053 748 Size: 2305 3717 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 2429 5898 2429 Size: 3469 2553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5136 5320 1305 Size: 3831 184 2007 Fragments arranged by size: 3831 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2313 10 Size: 54 1425 343 481 3719 Fragments arranged by size: 3719 1425 481 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2295 2368 3194 3283 3980 4339 4498 Size: 648 1129 73 826 89 697 359 159 Cuts at: 4498 518 Size: 2042 Fragments arranged by size: 2042 1129 826 697 648 359 159 89 73 MspA1I CmG'CkG Cuts at: 1606 2500 2858 2959 3438 4115 5263 5508 1606 Size: 894 358 101 479 677 1148 245 2120 Fragments arranged by size: 2120 1148 894 677 479 358 245 101 NaeI GCC'GGC Cuts at: 2561 2561 Size: 6022 NcoI C'CATG_G Cuts at: 513 1065 2004 2126 2308 3104 513 Size: 552 939 122 182 796 3431 Fragments arranged by size: 3431 939 796 552 182 122 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NgoMIV G'CCGG_C Cuts at: 2559 2559 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NsiI A_TGCA'T Cuts at: 3215 3215 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 2232 3188 3217 3380 4925 1132 Size: 97 1003 956 29 163 1545 2229 Fragments arranged by size: 2229 1545 1003 956 163 97 29 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 3105 1108 Size: 1997 4025 PfoI T'CCnGG_A Cuts at: 2072 2072 Size: 6022 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2666 2666 Size: 6022 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PspOMI G'GGCC_C Cuts at: 2122 2122 Size: 6022 PstI C_TGCA'G Cuts at: 830 3374 830 Size: 2544 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 PvuII CAG'CTG Cuts at: 2500 2959 2500 Size: 459 5563 SacI G_AGCT'C Cuts at: 2275 2275 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 3370 4505 5186 5377 826 Size: 219 2325 1135 681 191 1471 Fragments arranged by size: 2325 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 3349 1872 Size: 1477 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 4126 5027 5289 820 Size: 197 926 423 1760 901 262 1553 Fragments arranged by size: 1760 1553 926 901 423 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2004 2126 2308 3104 3461 513 Size: 552 939 122 182 796 357 3074 Fragments arranged by size: 3074 939 796 552 357 182 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4049 4066 4219 4404 4849 325 Size: 1255 2469 17 153 185 445 1498 Fragments arranged by size: 2469 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2864 3216 4268 Size: 274 80 33 51 1443 887 352 1052 Cuts at: 4268 5980 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1052 887 352 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2698 2730 2980 3012 5982 6014 2698 Size: 32 250 32 2970 32 2706 Fragments arranged by size: 2970 2706 250 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 3347 1870 Size: 1477 4545 XmnI GAAnn'nnTTC Cuts at: 2328 4151 2328 Size: 1823 4199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI