################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 41 GCGG CCGC(-3/-1) AlwNI 115 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 144 GGGCCC GGGCC^C AvaII 136 GGTCC G^G[AT]CC BamHI 4 GGATCC G^GATCC BanII 144 GGGCCC G[GA]GC[CT]^C BciVI 249 GTATCC GTATCC(6/5) BmyI 144 GGGCCC G[AGT]GC[ACT]^C BpmI 218 CTGGAG CTGGAG(16/14) BsiYI 166 CCTACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 166 CCTACAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 144 GGGCCC G[AGT]GC[ACT]^C BspHI 288 TCATGA T^CATGA BspMI 209 ACCTGC ACCTGC(4/8) BstYI 4 GGATCC [GA]^GATC[CT] Cac8I 279 GCTGGC GC[ACGT]^[ACGT]GC DdeI 259 CTCAG C^T[ACGT]AG DpnII 5 GATC ^GATC DraII 135 GGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 295 TGGCCG [CT]^GGCC[GA] EarI 70 GAAGAG CTCTTC(1/4) EcoO109I 135 GGGTCCT [GA]G^G[ACGT]CC[CT] HgaI 29 GCGTC GACGC(5/10) Ksp632I 70 GAAGAG CTCTTC(1/4) MboI 5 GATC ^GATC MboII 70 GAAGA GAAGA(8/7) MflI 4 GGATCC [GA]^GATC[CT] MseI 304 TTAA T^TAA NdeII 5 GATC ^GATC PpuMI 135 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 288 TCATGA T^CATGA Sau3AI 5 GATC ^GATC SfcI 167 CTACAG C^T[GA][CT]AG Tru9I 304 TTAA T^TAA Tsp45I 238 GTGAC ^GT[GC]AC TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 131 CCAAGGGTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 4 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwI 5 GATCC GGATC(4/5) 4 GGATC BsgI 13 GTGCAG GTGCAG(16/14) 90 GTGCAG BsmFI 34 GGGAC GGGAC(10/14) 163 GTCCC BsrI 293 ACTGG ACTGG(1/-1) 246 CCAGT BstF5I 102 CATCC GGATG(2/0) 149 CATCC EcoT14I 51 CCTTGG C^C[AT][AT]GG 131 CCAAGG FokI 102 CATCC GGATG(9/13) 149 CATCC MaeIII 176 GTTAC ^GT[ACGT]AC 238 GTGAC MspA1I 170 CAGCTG C[AC]G^C[GT]G 277 CAGCTG NlaIII 264 CATG CATG^ 289 CATG PvuII 170 CAGCTG CAG^CTG 277 CAGCTG StyI 51 CCTTGG C^C[AT][AT]GG 131 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI NlaIV Sau96I ScrFI TseI