Restriction map for
pFN21AE6544
(Circular) (Six-base) MAPSORT of: pFN21AE6544.seq Check: 3405 from: 1 to: 5026
pFN21AE6544 5026 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2836 278
Size: 53 83 186 936 1300 2468
Fragments arranged by size:
2468 1300 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2369 1937
Size: 432 4594
Acc65I G'GTAC_C
Cuts at: 2347 2347
Size: 5026
AclI AA'CG_TT
Cuts at: 1563 3153 3526 1563
Size: 1590 373 3063
Fragments arranged by size:
3063 1590 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3089 4473 4741 1632
Size: 1457 1384 268 1917
Fragments arranged by size:
1917 1457 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4829
AflIII A'CryG_T
Cuts at: 1203 3925 4902 1203
Size: 2722 977 1327
Fragments arranged by size:
2722 1327 977
AgeI A'CCGG_T
Cuts at: 3857 3857
Size: 5026
AhdI GACnn_n'nnGTC
Cuts at: 3755 3755
Size: 5026
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2217 2249 2217
Size: 32 4994
AlwNI CAG_nnn'CTG
Cuts at: 2125 2769 4341 2125
Size: 644 1572 2810
Fragments arranged by size:
2810 1572 644
ApaI G_GGCC'C
Cuts at: 2153 2153
Size: 5026
ApaLI G'TGCA_C
Cuts at: 3083 4239 3083
Size: 1156 3870
ApoI r'AATT_y
Cuts at: 1493 2335 2561 2713 4822 1493
Size: 842 226 152 2109 1697
Fragments arranged by size:
2109 1697 842 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3580 160
Size: 872 2548 1606
Fragments arranged by size:
2548 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5026
AvaI C'yCGr_G
Cuts at: 1870 1943 2351 1870
Size: 73 408 4545
Fragments arranged by size:
4545 408 73
BamHI G'GATC_C
Cuts at: 2009 2356 2723 2009
Size: 347 367 4312
Fragments arranged by size:
4312 367 347
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2347 3802 618
Size: 325 217 627 560 1455 1842
Fragments arranged by size:
1842 1455 627 560 325 217
BanII G_rGCy'C
Cuts at: 1391 2153 2345 1391
Size: 762 192 4072
Fragments arranged by size:
4072 762 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3217 3251 1903
Size: 34 1280 34 3678
Fragments arranged by size:
3678 1280 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2265 2918 4128 2265
Size: 653 1210 3163
Fragments arranged by size:
3163 1210 653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5026
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3637 136
Size: 107 122 71 631 605 1965 1525
Fragments arranged by size:
1965 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5021 5021
Size: 5026
BlpI GC'TnA_GC
Cuts at: 2443 2443
Size: 5026
Bme1580I G_kGCm'C
Cuts at: 1790 2153 3087 4243 1790
Size: 363 934 1156 2573
Fragments arranged by size:
2573 1156 934 363
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 2757 3715
Size: 416 53 83 399 24 828 512 958
Cuts at: 3715 442
Size: 1753
Fragments arranged by size:
1753 958 828 512 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 3686 1239
Size: 1005 1442 2579
Fragments arranged by size:
2579 1442 1005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3151 4016 4314 3151
Size: 865 298 3863
Fragments arranged by size:
3863 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3689 882
Size: 617 2190 2219
Fragments arranged by size:
2219 2190 617
BsaAI yAC'GTr
Cuts at: 493 1204 2817 493
Size: 711 1613 2702
Fragments arranged by size:
2702 1613 711
BsaBI GATnn'nnATC
Cuts at: 5020 5020
Size: 5026
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2833 3215 3850 275
Size: 53 83 186 936 1300 382 635 1451
Fragments arranged by size:
1451 1300 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3459 3857 4131 4278 4689 3459
Size: 398 274 147 411 3796
Fragments arranged by size:
3796 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2217 2247 1178
Size: 30 165 30 771 30 13 30 3957
Fragments arranged by size:
3957 771 165 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5026
BseYI C'CCAG_C
Cuts at: 4229 4725 4229
Size: 496 4530
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2116 1163
Size: 573 303 77 4073
Fragments arranged by size:
4073 573 303 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2476 3237 4265 1540
Size: 275 186 475 761 1028 2301
Fragments arranged by size:
2301 1028 761 475 275 186
BsiHKAI G_wGCw'C
Cuts at: 3087 3172 4243 3087
Size: 85 1071 3870
Fragments arranged by size:
3870 1071 85
BsmI GAATG_Cn'
Cuts at: 2537 2630 2537
Size: 93 4933
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2345 3087 3172 4243 1391
Size: 399 363 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 192 85
BspEI T'CCGG_A
Cuts at: 4689 4689
Size: 5026
BspHI T'CATG_A
Cuts at: 2293 2915 4645 2293
Size: 622 1730 2674
Fragments arranged by size:
2674 1730 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2381 844
Size: 1379 158 3489
Fragments arranged by size:
3489 1379 158
BsrBI CCG'CTC
Cuts at: 1394 1823 2913 1394
Size: 429 1090 3507
Fragments arranged by size:
3507 1090 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3521 3695 65
Size: 1183 2273 174 1396
Fragments arranged by size:
2273 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3670 3857 1700
Size: 110 1860 187 2869
Fragments arranged by size:
2869 1860 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5026
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5026
BssSI C'ACGA_G
Cuts at: 3086 4098 3086
Size: 1012 4014
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5026
BstBI TT'CG_AA
Cuts at: 3830 3830
Size: 5026
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5026
BstYI r'GATC_y
Cuts at: 1972 2009 2356 2723 3108 3125 4577 4663 4675
Size: 37 347 367 385 17 1452 86 12
Cuts at: 4675 5021 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 347 346 86 37
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3535
Fragments arranged by size:
3535 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4287
Fragments arranged by size:
4287 683 56
BtsI GCAGTG_nn'
Cuts at: 2542 3336 3356 2542
Size: 794 20 4212
Fragments arranged by size:
4212 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5026
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4991
DraI TTT'AAA
Cuts at: 2330 2330
Size: 5026
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4033 809
Size: 507 2717 1802
Fragments arranged by size:
2717 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2300 2473 3362
Size: 54 1425 50 275 18 470 173 889
Cuts at: 3362 4991 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 470 275 173 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2473 1537
Size: 275 661 4090
Fragments arranged by size:
4090 661 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2956 1353
Size: 717 886 3423
Fragments arranged by size:
3423 886 717
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3613 3987 4133 387
Size: 1064 138 2024 374 146 1280
Fragments arranged by size:
2024 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 3089 3686 4473 4741 1239
Size: 393 612 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 612 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2141 2141
Size: 5026
EcoRI G'AATT_C
Cuts at: 2335 2713 2335
Size: 378 4648
FspI TGC'GCA
Cuts at: 3532 3532
Size: 5026
HaeII r_GCGC'y
Cuts at: 1678 4173 1678
Size: 2495 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3667 3699 3741 3773 1373
Size: 32 135 32 2095 32 42 32 2626
Fragments arranged by size:
2626 2095 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2370 2616 669
Size: 988 281 432 246 3079
Fragments arranged by size:
3079 988 432 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5026
HpaI GTT'AAC
Cuts at: 2616 2616
Size: 5026
KpnI G_GTAC'C
Cuts at: 2351 2351
Size: 5026
MfeI C'AATT_G
Cuts at: 2625 2625
Size: 5026
MluI A'CGCG_T
Cuts at: 4902 4902
Size: 5026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4140 4324 1305
Size: 2835 184 2007
Fragments arranged by size:
2835 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3204
Fragments arranged by size:
3204 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2984 3343 3502 518
Size: 648 1818 359 159 2042
Fragments arranged by size:
2042 1818 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2284 2442 3119 4267 4512 1606
Size: 571 107 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 571 245 158 107
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3535
Fragments arranged by size:
3535 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5026
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5026
NotI GC'GGCC_GC
Cuts at: 2473 2473
Size: 5026
NspI r_CATG'y
Cuts at: 1132 1229 2384 3929 1132
Size: 97 1155 1545 2229
Fragments arranged by size:
2229 1545 1155 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5026
PciI A'CATG_T
Cuts at: 3925 3925
Size: 5026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5026
PmeI GTTT'AAAC
Cuts at: 2330 2330
Size: 5026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2217 2249 3095 3127 4608 4640 2217
Size: 32 846 32 1481 32 2603
Fragments arranged by size:
2603 1481 846 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2141 2141
Size: 5026
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5026
PsiI TTA'TAA
Cuts at: 2596 2596
Size: 5026
PspOMI G'GGCC_C
Cuts at: 2149 2149
Size: 5026
PstI C_TGCA'G
Cuts at: 830 2378 830
Size: 1548 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5026
PvuII CAG'CTG
Cuts at: 2177 2284 2177
Size: 107 4919
SalI G'TCGA_C
Cuts at: 1936 2368 1936
Size: 432 4594
SbfI CC_TGCA'GG
Cuts at: 2378 2378
Size: 5026
ScaI AGT'ACT
Cuts at: 3274 4986 3274
Size: 1712 3314
SfcI C'TryA_G
Cuts at: 826 1045 2172 2374 3509 4190 4381 826
Size: 219 1127 202 1135 681 191 1471
Fragments arranged by size:
1471 1135 1127 681 219 202 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5026
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5026
SmaI CCC'GGG
Cuts at: 1872 2353 1872
Size: 481 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3130 4031 4293 820
Size: 197 926 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2817 493
Size: 2324 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5026
SphI G_CATG'C
Cuts at: 1132 2384 1132
Size: 1252 3774
SspI AAT'ATT
Cuts at: 5 52 2950 5
Size: 47 2898 2081
Fragments arranged by size:
2898 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5026
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2136 2465 513
Size: 552 939 52 80 329 3074
Fragments arranged by size:
3074 939 552 329 80 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2005 3053 3070 3223 3408 3853 325
Size: 1255 425 1048 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1048 445 425 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3272 4984 96
Size: 274 80 33 51 1443 1295 1712 138
Fragments arranged by size:
1712 1443 1295 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 4986 5018 2001
Size: 32 2953 32 2009
Fragments arranged by size:
2953 2009 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5026
XbaI T'CTAG_A
Cuts at: 2362 2362
Size: 5026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2143
Size: 5026
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5026
XmaI C'CCGG_G
Cuts at: 1870 2351 1870
Size: 481 4545
XmnI GAAnn'nnTTC
Cuts at: 3155 3155
Size: 5026
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2834 276
Size: 53 83 186 936 1300 2468
Fragments arranged by size:
2468 1300 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI