(Linear) (Six-base) MAPSORT of: KIEE6544.seq Check: 4697 from: 1 to: 321 KIEE6544.orf With 182 enzymes: SgfI * March 16, 2015 11:33 .. AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 212 244 321 Size: 212 32 77 Fragments arranged by size: 212 77 32 AlwNI CAG_nnn'CTG Cuts at: 0 120 321 Size: 120 201 ApaI G_GGCC'C Cuts at: 0 148 321 Size: 148 173 BamHI G'GATC_C Cuts at: 0 4 321 Size: 4 317 BanII G_rGCy'C Cuts at: 0 148 321 Size: 148 173 BciVI GTATCCnnnnn_n' Cuts at: 0 260 321 Size: 260 61 Bme1580I G_kGCm'C Cuts at: 0 148 321 Size: 148 173 BmrI ACTGGGnnnn_n' Cuts at: 0 240 321 Size: 240 81 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 321 Size: 239 82 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 212 242 321 Size: 169 30 13 30 79 Fragments arranged by size: 169 79 30 30 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 111 321 Size: 34 77 210 Fragments arranged by size: 210 77 34 Bsp1286I G_dGCh'C Cuts at: 0 148 321 Size: 148 173 BspHI T'CATG_A Cuts at: 0 288 321 Size: 288 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 218 321 Size: 218 103 BstYI r'GATC_y Cuts at: 0 4 321 Size: 4 317 EaeI y'GGCC_r Cuts at: 0 295 321 Size: 295 26 EarI CTCTTCn'nnn_ Cuts at: 0 65 321 Size: 65 256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 321 Size: 239 82 EcoO109I rG'GnC_Cy Cuts at: 0 136 321 Size: 136 185 MspA1I CmG'CkG Cuts at: 0 172 279 321 Size: 172 107 42 Fragments arranged by size: 172 107 42 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 212 244 321 Size: 212 32 77 Fragments arranged by size: 212 77 32 PpuMI rG'GwC_Cy Cuts at: 0 136 321 Size: 136 185 PspOMI G'GGCC_C Cuts at: 0 144 321 Size: 144 177 PvuII CAG'CTG Cuts at: 0 172 279 321 Size: 172 107 42 Fragments arranged by size: 172 107 42 SfcI C'TryA_G Cuts at: 0 167 321 Size: 167 154 StyI C'CwwG_G Cuts at: 0 51 131 321 Size: 51 80 190 Fragments arranged by size: 190 80 51 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 28 321 Size: 28 293 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 138 321 Size: 138 183 Enzymes that do cut: AloI AlwNI ApaI BamHI BanII BciVI Bme1580I BmrI BpmI BsaXI BsgI Bsp1286I BspHI BspMI BstYI EaeI EarI Eco57MI EcoO109I MspA1I PpiI PpuMI PspOMI PvuII SfcI StyI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI