Restriction map for
pFN21AE5774
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BbrPI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MunI MvnI
NaeI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 750 TCCGGA T^CCGGA
AcyI 117 GGCGCC G[GA]^CG[CT]C
AocI 615 CCTGAGG CC^T[ACGT]AGG
AspI 1203 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 117 GGCGCC G^G[CT][GA]CC
BbeI 117 GGCGCC GGCGC^C
BciVI 864 GTATCC GTATCC(6/5)
BsaHI 117 GGCGCC G[GA]^CG[CT]C
BsaWI 750 TCCGGA [AT]^CCGG[AT]
BseAI 750 TCCGGA T^CCGGA
BspEI 750 TCCGGA T^CCGGA
BspMI 1591 ACCTGC ACCTGC(4/8)
BstXI 173 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1442 GGATCT [GA]^GATC[CT]
Bsu36I 615 CCTGAGG CC^T[ACGT]AGG
CfoI 118 GCGC GCG^C
DraI 135 TTTAAA TTT^AAA
Eco81I 615 CCTGAGG CC^T[ACGT]AGG
FauI 1706 CCCGC CCCGC(4/6)
HaeII 117 GGCGCC [GA]GCGC^[CT]
HapII 751 CCGG C^CGG
HhaI 118 GCGC GCG^C
Hin1I 117 GGCGCC G[GA]^CG[CT]C
HinP1I 118 GCGC G^CGC
HpaII 751 CCGG C^CGG
KasI 117 GGCGCC G^GCGCC
MflI 1442 GGATCT [GA]^GATC[CT]
MroI 750 TCCGGA T^CCGGA
MspI 751 CCGG C^CGG
NarI 117 GGCGCC GG^CGCC
PflFI 1203 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 241 CTGCAG CTGCA^G
SacI 1636 GAGCTC GAGCT^C
SfcI 241 CTGCAG C^T[GA][CT]AG
SfoI 117 GGCGCC GGC^GCC
Tth111I 1203 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 717 TCTAGA T^CTAGA
XhoII 1442 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 816 GATCC GGATC(4/5)
1442 GGATC
AspHI 1636 GAGCTC G[AT]GC[AT]^C
1846 GAGCAC
BalI 608 TGGCCA TGG^CCA
1154 TGGCCA
BbsI 972 GAAGAC GAAGAC(2/6)
1435 GAAGAC
BbvCI 385 GCTGAGG CCTCAGC(-5/-2)
679 GCTGAGG
BpuAI 972 GAAGAC GAAGAC(2/6)
1435 GAAGAC
BsaI 946 GAGACC GGTCTC(1/5)
1294 GGTCTC
BseRI 434 GAGGAG GAGGAG(10/8)
1633 GAGGAG
BsiHKAI 1636 GAGCTC G[AT]GC[AT]^C
1846 GAGCAC
DraII 939 AGGTCCT [GA]G^G[ACGT]CC[CT]
1702 GGGACCC
EaeI 608 TGGCCA [CT]^GGCC[GA]
1154 TGGCCA
EcoO109I 939 AGGTCCT [GA]G^G[ACGT]CC[CT]
1702 GGGACCC
EcoT14I 950 CCAAGG C^C[AT][AT]GG
1853 CCAAGG
HincII 163 GTCAAC GT[CT]^[GA]AC
187 GTCAAC
HindII 163 GTCAAC GT[CT]^[GA]AC
187 GTCAAC
MluNI 608 TGGCCA TGG^CCA
1154 TGGCCA
MscI 608 TGGCCA TGG^CCA
1154 TGGCCA
MspA1I 174 CAGCTG C[AC]G^C[GT]G
383 CAGCTG
NdeI 471 CATATG CA^TATG
1289 CATATG
PflMI 687 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCACGAACTGG
PpuMI 939 AGGTCCT [GA]G^G[AT]CC[CT]
1702 GGGACCC
PvuII 174 CAGCTG CAG^CTG
383 CAGCTG
StyI 950 CCAAGG C^C[AT][AT]GG
1853 CCAAGG
TaqI 150 TCGA T^CGA
1687 TCGA
TthHB8I 150 TCGA T^CGA
1687 TCGA
Van91I 687 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
803 CCACGAACTGG
XcmI 1050 CCAAGCTGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1466 CCAGTGACAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StuI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI