Restriction map for
pFN21AE5774
(Circular) (Six-base) MAPSORT of: pFN21AE5774.seq Check: 860 from: 1 to: 6571
pFN21AE5774 6571 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3081 3431 3589 4634 6018 6286 1632
Size: 1449 350 158 1045 1384 268 1917
Fragments arranged by size:
1917 1449 1384 1045 350 268 158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2733 1184
Size: 1549 5022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2285 2317 2285
Size: 32 6539
AlwNI CAG_nnn'CTG
Cuts at: 4314 5886 4314
Size: 1572 4999
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3199 3528 3805 3824 3880 4106 4258 6367
Size: 1706 329 277 19 56 226 152 2109
Cuts at: 6367 1493
Size: 1697
Fragments arranged by size:
2109 1706 1697 329 277 226 152 56
19
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 3896 1870
Size: 73 1953 4545
Fragments arranged by size:
4545 1953 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3892 5347 618
Size: 325 217 627 335 1770 1455 1842
Fragments arranged by size:
1842 1770 1455 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2446 2775 3645 3787 3890 1391
Size: 1055 329 870 142 103 4072
Fragments arranged by size:
4072 1055 870 329 142 103
BbeI G_GCGC'C
Cuts at: 2126 2126
Size: 6571
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2984 3447 928
Size: 971 1085 463 4052
Fragments arranged by size:
4052 1085 971 463
BbvCI CC'TCA_GC
Cuts at: 2391 2685 2391
Size: 294 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2880 4463 5673 2880
Size: 1583 1210 3778
Fragments arranged by size:
3778 1583 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5182 136
Size: 107 122 71 631 605 3510 1525
Fragments arranged by size:
3510 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 4632 5788 1790
Size: 2842 1156 2573
Fragments arranged by size:
2842 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2728 1318
Size: 1410 5161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 3465 3641
Size: 416 53 83 399 24 1024 1024 176
Cuts at: 3641 4302 5260 442
Size: 661 958 1753
Fragments arranged by size:
1753 1024 1024 958 661 416 399 176
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 2326 2335 2592 3137 5231 1239
Size: 796 291 9 257 545 2094 2579
Fragments arranged by size:
2579 2094 796 545 291 257 9
Bpu10I CC'TnA_GC
Cuts at: 2391 2685 2391
Size: 294 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2945 3305 5234 882
Size: 617 1446 360 1929 2219
Fragments arranged by size:
2219 1929 1446 617 360
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 4378 4760 5395
Size: 53 83 186 936 590 2255 382 635
Cuts at: 5395 275
Size: 1451
Fragments arranged by size:
2255 1451 936 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2755 5004 5402 5676 5823 6234 2755
Size: 2249 398 274 147 411 3092
Fragments arranged by size:
3092 2249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 2433 2463 3855
Size: 30 165 30 882 30 118 30 1392
Cuts at: 3855 3885 1178
Size: 30 3864
Fragments arranged by size:
3864 1392 882 165 118 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2454 3653 1186
Size: 1268 1199 4104
Fragments arranged by size:
4104 1268 1199
BseYI C'CCAG_C
Cuts at: 3173 5774 6270 3173
Size: 2601 496 3474
Fragments arranged by size:
3474 2601 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5998
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4021 4782 5810 1540
Size: 275 186 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3645 3855 4632 4717 5788 3645
Size: 210 777 85 1071 4428
Fragments arranged by size:
4428 1071 777 210 85
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 2775 3645 3787 3855 3890 4632
Size: 399 656 329 870 142 68 35 742
Cuts at: 4632 4717 5788 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 656 399 329 142
85 68 35
BspEI T'CCGG_A
Cuts at: 2755 6234 2755
Size: 3479 3092
BspHI T'CATG_A
Cuts at: 4460 6190 4460
Size: 1730 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3605 3926 844
Size: 2761 321 3489
Fragments arranged by size:
3489 2761 321
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5215 5402 1700
Size: 110 3405 187 2869
Fragments arranged by size:
3405 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6571
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6571
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2185
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 3447 3901 4268 4653 4670 6122 6208 6220
Size: 1475 454 367 385 17 1452 86 12
Cuts at: 6220 6566 1972
Size: 346 1977
Fragments arranged by size:
1977 1475 1452 454 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2621 2621
Size: 6571
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2293 487
Size: 683 56 1067 4765
Fragments arranged by size:
4765 1067 683 56
BtsI GCAGTG_nn'
Cuts at: 4087 4881 4901 4087
Size: 794 20 5757
Fragments arranged by size:
5757 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 2142 3875 2142
Size: 1733 4838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2613 3159 4018
Size: 54 1425 50 275 18 783 546 859
Cuts at: 4018 4907 6536 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 859 783 546 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4501 1353
Size: 3148 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2411 3654 5158 5532 5678 387
Size: 1064 138 822 1243 1504 374 146 1280
Fragments arranged by size:
1504 1280 1243 1064 822 374 146 138
EcoICRI GAG'CTC
Cuts at: 3643 3643
Size: 6571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2326 2335 2592 3081 3137 3431
Size: 393 403 291 9 257 489 56 294
Cuts at: 3431 3589 4634 5231 6018 6286 1239
Size: 158 1045 597 787 268 1524
Fragments arranged by size:
1524 1045 787 597 489 403 393 294
291 268 257 158 56 9
EcoO109I rG'GnC_Cy
Cuts at: 2945 3708 2945
Size: 763 5808
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3749 3749
Size: 6571
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 2126 5718 1678
Size: 448 3592 2531
Fragments arranged by size:
3592 2531 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 2433 2465 2750
Size: 32 135 32 699 32 130 32 285
Cuts at: 2750 2782 5212 5244 5286 5318 1373
Size: 32 2430 32 42 32 2626
Fragments arranged by size:
2626 2430 699 285 135 130 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 2194 3915 4161 669
Size: 988 281 232 24 1721 246 3079
Fragments arranged by size:
3079 1721 988 281 246 232 24
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KasI G'GCGC_C
Cuts at: 2122 2122
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5685 5869 1305
Size: 4380 184 2007
Fragments arranged by size:
4380 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 3161 10
Size: 54 1425 343 783 546 3420
Fragments arranged by size:
3420 1425 783 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2653 2738 2864 2972 3384 3771 4529
Size: 648 1487 85 126 108 412 387 758
Cuts at: 4529 4888 5047 518
Size: 359 159 2042
Fragments arranged by size:
2042 1487 758 648 412 387 359 159
126 108 85
MspA1I CmG'CkG
Cuts at: 1606 2181 2390 3987 4664 5812 6057 1606
Size: 575 209 1597 677 1148 245 2120
Fragments arranged by size:
2120 1597 1148 677 575 245 209
NarI GG'CG_CC
Cuts at: 2123 2123
Size: 6571
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6019
NdeI CA'TA_TG
Cuts at: 387 2477 3295 387
Size: 2090 818 3663
Fragments arranged by size:
3663 2090 818
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3929 5474 1132
Size: 97 2700 1545 2229
Fragments arranged by size:
2700 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3622 1348
Size: 2274 4297
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2698 2814 1108
Size: 1590 116 4865
Fragments arranged by size:
4865 1590 116
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2285 2317 4640 4672 6153 6185 2285
Size: 32 2323 32 1481 32 2671
Fragments arranged by size:
2671 2323 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2945 3708 2945
Size: 763 5808
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 2589 4141 2589
Size: 1552 5019
PstI C_TGCA'G
Cuts at: 830 2250 3923 830
Size: 1420 1673 3478
Fragments arranged by size:
3478 1673 1420
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2181 2390 2181
Size: 209 6362
SacI G_AGCT'C
Cuts at: 3645 3645
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SanDI GG'GwC_CC
Cuts at: 3708 3708
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SfcI C'TryA_G
Cuts at: 826 1045 2246 3919 5054 5735 5926 826
Size: 219 1201 1673 1135 681 191 1471
Fragments arranged by size:
1673 1471 1201 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2124 2124
Size: 6571
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4675 5576 5838 820
Size: 197 926 2732 901 262 1553
Fragments arranged by size:
2732 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2675 3264 3610 1517
Size: 1158 589 346 4478
Fragments arranged by size:
4478 1158 589 346
StyI C'CwwG_G
Cuts at: 513 1065 2955 3858 4010 513
Size: 552 1890 903 152 3074
Fragments arranged by size:
3074 1890 903 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2560 4598 4615 4768 4953 5398 325
Size: 1255 980 2038 17 153 185 445 1498
Fragments arranged by size:
2038 1498 1255 980 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4817 6529 96
Size: 274 80 33 51 1443 2840 1712 138
Fragments arranged by size:
2840 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6531 6563 6531
Size: 32 6539
Tth111I GACn'n_nGTC
Cuts at: 1316 3211 1316
Size: 1895 4676
XbaI T'CTAG_A
Cuts at: 2722 3907 2722
Size: 1185 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 3478 3062
Size: 416 6155
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6571
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 2751 2931 3843 4700 2751
Size: 180 912 857 4622
Fragments arranged by size:
4622 912 857 180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI