(Linear) (Six-base) MAPSORT of: KIEE5774.seq Check: 5393 from: 1 to: 1866 KIEE5774.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1076 1426 1584 1866 Size: 1076 350 158 282 Fragments arranged by size: 1076 350 282 158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 728 1866 Size: 728 1138 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 280 312 1866 Size: 280 32 1554 Fragments arranged by size: 1554 280 32 ApoI r'AATT_y Cuts at: 0 1194 1523 1800 1819 1866 Size: 1194 329 277 19 47 Fragments arranged by size: 1194 329 277 47 19 BanI G'GyrC_C Cuts at: 0 117 1866 Size: 117 1749 BanII G_rGCy'C Cuts at: 0 441 770 1640 1782 1866 Size: 441 329 870 142 84 Fragments arranged by size: 870 441 329 142 84 BbeI G_GCGC'C Cuts at: 0 121 1866 Size: 121 1745 BbsI GAAGACnn'nnnn_ Cuts at: 0 979 1442 1866 Size: 979 463 424 Fragments arranged by size: 979 463 424 BbvCI CC'TCA_GC Cuts at: 0 386 680 1866 Size: 386 294 1186 Fragments arranged by size: 1186 386 294 BciVI GTATCCnnnnn_n' Cuts at: 0 875 1866 Size: 875 991 BmgBI CAC'GTC Cuts at: 0 723 1866 Size: 723 1143 BmrI ACTGGGnnnn_n' Cuts at: 0 436 1460 1636 1866 Size: 436 1024 176 230 Fragments arranged by size: 1024 436 230 176 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 321 330 587 1132 1866 Size: 30 291 9 257 545 734 Fragments arranged by size: 734 545 291 257 30 9 Bpu10I CC'TnA_GC Cuts at: 0 386 680 1866 Size: 386 294 1186 Fragments arranged by size: 1186 386 294 BsaI GGTCTCn'nnnn_ Cuts at: 0 940 1300 1866 Size: 940 360 566 Fragments arranged by size: 940 566 360 BsaHI Gr'CG_yC Cuts at: 0 118 1866 Size: 118 1748 BsaWI w'CCGG_w Cuts at: 0 750 1866 Size: 750 1116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 280 310 428 458 1866 Size: 280 30 118 30 1408 Fragments arranged by size: 1408 280 118 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 449 1648 1866 Size: 449 1199 218 Fragments arranged by size: 1199 449 218 BseYI C'CCAG_C Cuts at: 0 1168 1866 Size: 1168 698 BsiHKAI G_wGCw'C Cuts at: 0 1640 1850 1866 Size: 1640 210 16 Fragments arranged by size: 1640 210 16 Bsp1286I G_dGCh'C Cuts at: 0 441 770 1640 1782 1850 1866 Size: 441 329 870 142 68 16 Fragments arranged by size: 870 441 329 142 68 16 BspEI T'CCGG_A Cuts at: 0 750 1866 Size: 750 1116 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1600 1866 Size: 1600 266 BstXI CCAn_nnnn'nTGG Cuts at: 0 180 1866 Size: 180 1686 BstYI r'GATC_y Cuts at: 0 1442 1866 Size: 1442 424 Bsu36I CC'TnA_GG Cuts at: 0 616 1866 Size: 616 1250 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 288 1866 Size: 288 1578 DraI TTT'AAA Cuts at: 0 137 1866 Size: 137 1729 EaeI y'GGCC_r Cuts at: 0 608 1154 1866 Size: 608 546 712 Fragments arranged by size: 712 608 546 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 406 1649 1866 Size: 406 1243 217 Fragments arranged by size: 1243 406 217 EcoICRI GAG'CTC Cuts at: 0 1638 1866 Size: 1638 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 321 330 587 1076 1132 1426 1584 Size: 30 291 9 257 489 56 294 158 Cuts at: 1584 1866 Size: 282 Fragments arranged by size: 489 294 291 282 257 158 56 30 9 EcoO109I rG'GnC_Cy Cuts at: 0 940 1703 1866 Size: 940 763 163 Fragments arranged by size: 940 763 163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1744 1866 Size: 1744 122 HaeII r_GCGC'y Cuts at: 0 121 1866 Size: 121 1745 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 266 298 428 460 745 777 1866 Size: 266 32 130 32 285 32 1089 Fragments arranged by size: 1089 285 266 130 32 32 32 HincII GTy'rAC Cuts at: 0 165 189 1866 Size: 165 24 1677 Fragments arranged by size: 1677 165 24 KasI G'GCGC_C Cuts at: 0 117 1866 Size: 117 1749 MscI TGG'CCA Cuts at: 0 610 1156 1866 Size: 610 546 710 Fragments arranged by size: 710 610 546 MslI CAynn'nnrTG Cuts at: 0 648 733 859 967 1379 1766 1866 Size: 648 85 126 108 412 387 100 Fragments arranged by size: 648 412 387 126 108 100 85 MspA1I CmG'CkG Cuts at: 0 176 385 1866 Size: 176 209 1481 Fragments arranged by size: 1481 209 176 NarI GG'CG_CC Cuts at: 0 118 1866 Size: 118 1748 NdeI CA'TA_TG Cuts at: 0 472 1290 1866 Size: 472 818 576 Fragments arranged by size: 818 576 472 PasI CC'CwG_GG Cuts at: 0 1617 1866 Size: 1617 249 PflMI CCAn_nnn'nTGG Cuts at: 0 693 809 1866 Size: 693 116 1057 Fragments arranged by size: 1057 693 116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 280 312 1866 Size: 280 32 1554 Fragments arranged by size: 1554 280 32 PpuMI rG'GwC_Cy Cuts at: 0 940 1703 1866 Size: 940 763 163 Fragments arranged by size: 940 763 163 PsiI TTA'TAA Cuts at: 0 584 1866 Size: 584 1282 PstI C_TGCA'G Cuts at: 0 245 1866 Size: 245 1621 PvuII CAG'CTG Cuts at: 0 176 385 1866 Size: 176 209 1481 Fragments arranged by size: 1481 209 176 SacI G_AGCT'C Cuts at: 0 1640 1866 Size: 1640 226 SanDI GG'GwC_CC Cuts at: 0 1703 1866 Size: 1703 163 SfcI C'TryA_G Cuts at: 0 241 1866 Size: 241 1625 SfoI GGC'GCC Cuts at: 0 119 1866 Size: 119 1747 StuI AGG'CCT Cuts at: 0 670 1259 1605 1866 Size: 670 589 346 261 Fragments arranged by size: 670 589 346 261 StyI C'CwwG_G Cuts at: 0 950 1853 1866 Size: 950 903 13 Fragments arranged by size: 950 903 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 555 1866 Size: 555 1311 Tth111I GACn'n_nGTC Cuts at: 0 1206 1866 Size: 1206 660 XbaI T'CTAG_A Cuts at: 0 717 1866 Size: 717 1149 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1057 1473 1866 Size: 1057 416 393 Fragments arranged by size: 1057 416 393 XmnI GAAnn'nnTTC Cuts at: 0 746 926 1838 1866 Size: 746 180 912 28 Fragments arranged by size: 912 746 180 28 Enzymes that do cut: AcuI AlfI AloI ApoI BanI BanII BbeI BbsI BbvCI BciVI BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BstXI BstYI Bsu36I BtgZI DraI EaeI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MscI MslI MspA1I NarI NdeI PasI PflMI PpiI PpuMI PsiI PstI PvuII SacI SanDI SfcI SfoI StuI StyI TaqII Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpuEI BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtsI ClaI CspCI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XhoI XmaI ZraI