Restriction map for
pFN21AE6783
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BcgI BciVI BfrI BglI BlnI
BsaBI BseAI BsiWI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssSI Bst1107I BstBI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FseI
FspI HgaI HindIII KpnI Ksp632I
KspI MamI MfeI MluI MroI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 229 GGCGCC G[GA]^CG[CT]C
ApaI 648 GGGCCC GGGCC^C
BalI 1244 TGGCCA TGG^CCA
BamHI 762 GGATCC G^GATCC
BbeI 229 GGCGCC GGCGC^C
BbvCI 1632 GCTGAGG CCTCAGC(-5/-2)
BclI 1232 TGATCA T^GATCA
BfaI 1459 CTAG C^TAG
BglII 15 AGATCT A^GATCT
BsaHI 229 GGCGCC G[GA]^CG[CT]C
BsaWI 137 TCCGGT [AT]^CCGG[AT]
BsiEI 1689 CGACCG CG[GA][CT]^CG
BsmBI 678 GAGACG CGTCTC(1/5)
Bsp1407I 883 TGTACA T^GTACA
BsrBI 1294 CCGCTC CCGCTC(-3/-3)
BsrGI 883 TGTACA T^GTACA
BssHII 190 GCGCGC G^CGCGC
BstEII 1161 GGTGACC G^GT[ACGT]ACC
BstPI 1161 GGTGACC G^GT[ACGT]ACC
EcoO65I 1161 GGTGACC G^GT[ACGT]ACC
EcoT14I 1304 CCAAGG C^C[AT][AT]GG
FauI 1742 GCGGG CCCGC(4/6)
FbaI 1232 TGATCA T^GATCA
HaeII 229 GGCGCC [GA]GCGC^[CT]
Hin1I 229 GGCGCC G[GA]^CG[CT]C
HpaI 1653 GTTAAC GTT^AAC
KasI 229 GGCGCC G^GCGCC
MaeI 1459 CTAG C^TAG
MluNI 1244 TGGCCA TGG^CCA
MscI 1244 TGGCCA TGG^CCA
NarI 229 GGCGCC GG^CGCC
NspI 1750 GCATGC [GA]CATG^[CT]
PflMI 1796 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1348 GAGCTC GAGCT^C
SfoI 229 GGCGCC GGC^GCC
SphI 1750 GCATGC GCATG^C
SspBI 883 TGTACA T^GTACA
StuI 983 AGGCCT AGG^CCT
StyI 1304 CCAAGG C^C[AT][AT]GG
TfiI 801 GAATC G^A[AT]TC
Van91I 1796 CCAATCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 653 CCAGGCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 306 GTGCAC G^TGCAC
728 GTGCAC
AocI 282 CCTGAGG CC^T[ACGT]AGG
1566 CCTGAGG
ApaLI 306 GTGCAC G^TGCAC
728 GTGCAC
BanII 648 GGGCCC G[GA]GC[CT]^C
1348 GAGCTC
BbrPI 586 CACGTG CAC^GTG
1248 CACGTG
BsaAI 586 CACGTG [CT]AC^GT[GA]
1248 CACGTG
BseRI 94 GAGGAG GAGGAG(10/8)
97 GAGGAG
BstAPI 308 GCACGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
417 GCAGCTCTTGC
Bsu36I 282 CCTGAGG CC^T[ACGT]AGG
1566 CCTGAGG
DraIII 253 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
309 CACGGTGTG
DrdI 325 GACAGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
694 GACACTGCGGTC
EaeI 1244 TGGCCA [CT]^GGCC[GA]
1483 CGGCCA
Eco81I 282 CCTGAGG CC^T[ACGT]AGG
1566 CCTGAGG
EcoT22I 975 ATGCAT ATGCA^T
1748 ATGCAT
HphI 1161 GGTGA GGTGA(8/7)
710 TCACC
NsiI 975 ATGCAT ATGCA^T
1748 ATGCAT
PleI 1815 GACTC GAGTC(4/5)
1091 GAGTC
PmaCI 586 CACGTG CAC^GTG
1248 CACGTG
PmlI 586 CACGTG CAC^GTG
1248 CACGTG
PpuMI 59 AGGTCCT [GA]G^G[AT]CC[CT]
289 GGGACCC
SmlI 28 CTCAAG C^T[CT][GA]AG
1406 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII