(Circular) (Six-base) MAPSORT of: pFN21AE6783.seq Check: 5533 from: 1 to: 6535 pFN21AE6783 6535 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2139 2197 2139 Size: 58 6477 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4345 278 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3878 1937 Size: 1941 4594 Acc65I G'GTAC_C Cuts at: 3856 3856 Size: 6535 AclI AA'CG_TT Cuts at: 1563 4662 5035 1563 Size: 3099 373 3063 Fragments arranged by size: 3099 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 3598 4598 5982 6250 1632 Size: 1946 20 1000 1384 268 1917 Fragments arranged by size: 1946 1917 1384 1000 268 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6338 AflIII A'CryG_T Cuts at: 1203 5434 6411 1203 Size: 4231 977 1327 Fragments arranged by size: 4231 1327 977 AgeI A'CCGG_T Cuts at: 5366 5366 Size: 6535 AhdI GACnn_n'nnGTC Cuts at: 5264 5264 Size: 6535 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6535 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6535 AlwNI CAG_nnn'CTG Cuts at: 2068 2212 2446 2886 4278 5850 2068 Size: 144 234 440 1392 1572 2753 Fragments arranged by size: 2753 1572 1392 440 234 144 ApaI G_GGCC'C Cuts at: 2657 2657 Size: 6535 ApaLI G'TGCA_C Cuts at: 2311 2733 4592 5748 2311 Size: 422 1859 1156 3098 Fragments arranged by size: 3098 1859 1156 422 ApoI r'AATT_y Cuts at: 1493 2384 2454 2528 2566 2936 3475 3844 4070 Size: 891 70 74 38 370 539 369 226 Cuts at: 4070 4222 6331 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 891 539 370 369 226 152 74 70 38 AseI AT'TA_AT Cuts at: 160 1032 5089 160 Size: 872 4057 1606 Fragments arranged by size: 4057 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 AvaI C'yCGr_G Cuts at: 1870 1943 3860 1870 Size: 73 1917 4545 Fragments arranged by size: 4545 1917 73 BamHI G'GATC_C Cuts at: 2767 3865 4232 2767 Size: 1098 367 5070 Fragments arranged by size: 5070 1098 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2824 3497 3856 5311 Size: 325 217 627 447 590 673 359 1455 Cuts at: 5311 618 Size: 1842 Fragments arranged by size: 1842 1455 673 627 590 447 359 325 217 BanII G_rGCy'C Cuts at: 1391 2657 3357 3854 1391 Size: 1266 700 497 4072 Fragments arranged by size: 4072 1266 700 497 BbeI G_GCGC'C Cuts at: 2238 2238 Size: 6535 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3053 3273 3333 3604 928 Size: 971 1154 220 60 271 3859 Fragments arranged by size: 3859 1154 971 271 220 60 BbvCI CC'TCA_GC Cuts at: 3638 3638 Size: 6535 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4726 4760 1903 Size: 34 2789 34 3678 Fragments arranged by size: 3678 2789 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4427 5637 4427 Size: 1210 5325 BclI T'GATC_A Cuts at: 1548 3237 1548 Size: 1689 4846 BfrBI ATG'CAT Cuts at: 2982 3755 2982 Size: 773 5762 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5146 136 Size: 107 122 71 631 605 3474 1525 Fragments arranged by size: 3474 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2020 6530 2020 Size: 4510 2025 BlpI GC'TnA_GC Cuts at: 2447 3369 3633 3952 2447 Size: 922 264 319 5030 Fragments arranged by size: 5030 922 319 264 Bme1580I G_kGCm'C Cuts at: 1790 2272 2315 2657 2737 3206 4596 5752 1790 Size: 482 43 342 80 469 1390 1156 2573 Fragments arranged by size: 2573 1390 1156 482 469 342 80 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 2266 2823 Size: 416 53 83 399 24 762 87 557 Cuts at: 2823 3586 4266 5224 442 Size: 763 680 958 1753 Fragments arranged by size: 1753 958 763 762 680 557 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6535 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2441 2441 Size: 6535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2373 2415 2865 2946 3114 3758 5195 Size: 806 328 42 450 81 168 644 1437 Cuts at: 5195 1239 Size: 2579 Fragments arranged by size: 2579 1437 806 644 450 328 168 81 42 Bpu10I CC'TnA_GC Cuts at: 3638 3638 Size: 6535 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2018 3396 4660 5525 5823 2018 Size: 1378 1264 865 298 2730 Fragments arranged by size: 2730 1378 1264 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2070 3125 3190 5198 882 Size: 617 571 1055 65 2008 2219 Fragments arranged by size: 2219 2008 1055 617 571 65 BsaAI yAC'GTr Cuts at: 493 1204 2593 3255 4326 493 Size: 711 1389 662 1071 2702 Fragments arranged by size: 2702 1389 1071 711 662 BsaBI GATnn'nnATC Cuts at: 6529 6529 Size: 6535 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2235 4342 4724 5359 Size: 53 83 186 936 702 2107 382 635 Cuts at: 5359 275 Size: 1451 Fragments arranged by size: 2107 1451 936 702 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 4968 5366 5640 5787 6198 2142 Size: 2826 398 274 147 411 2479 Fragments arranged by size: 2826 2479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2464 2494 2793 2823 3056 Size: 30 165 30 1061 30 299 30 233 Cuts at: 3056 3086 1178 Size: 30 4627 Fragments arranged by size: 4627 1061 299 233 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2117 1186 Size: 928 3 5604 Fragments arranged by size: 5604 928 3 BseYI C'CCAG_C Cuts at: 2212 3159 3628 5738 6234 2212 Size: 947 469 2110 496 2513 Fragments arranged by size: 2513 2110 947 496 469 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 2521 3815 1163 Size: 573 474 311 1294 3883 Fragments arranged by size: 3883 1294 573 474 311 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 3985 4746 5774 1540 Size: 275 186 1696 288 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 288 275 186 BsiHKAI G_wGCw'C Cuts at: 2032 2178 2315 2737 3357 4596 4681 5752 2032 Size: 146 137 422 620 1239 85 1071 2815 Fragments arranged by size: 2815 1239 1071 620 422 146 137 85 BsmI GAATG_Cn' Cuts at: 4046 4139 4046 Size: 93 6442 BsmBI CGTCTCn'nnnn_ Cuts at: 2677 2677 Size: 6535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2178 2272 2315 2657 2737 3206 Size: 399 242 146 94 43 342 80 469 Cuts at: 3206 3357 3854 4596 4681 5752 1391 Size: 151 497 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 497 469 399 342 242 151 146 94 85 80 43 BspEI T'CCGG_A Cuts at: 6198 6198 Size: 6535 BspHI T'CATG_A Cuts at: 4424 6154 4424 Size: 1730 4805 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2197 2508 3172 3890 844 Size: 1295 58 311 664 718 3489 Fragments arranged by size: 3489 1295 718 664 311 58 BsrBI CCG'CTC Cuts at: 1394 1823 3301 4422 1394 Size: 429 1478 1121 3507 Fragments arranged by size: 3507 1478 1121 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5030 5204 65 Size: 1183 3782 174 1396 Fragments arranged by size: 3782 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5179 5366 1700 Size: 110 3369 187 2869 Fragments arranged by size: 3369 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2888 96 Size: 2792 3743 BssHII G'CGCG_C Cuts at: 1925 2195 1925 Size: 270 6265 BssSI C'ACGA_G Cuts at: 4595 5607 4595 Size: 1012 5523 BstAPI GCAn_nnn'nTGC Cuts at: 794 2319 2428 794 Size: 1525 109 4901 Fragments arranged by size: 4901 1525 109 BstBI TT'CG_AA Cuts at: 5339 5339 Size: 6535 BstEII G'GTnAC_C Cuts at: 1184 3166 1184 Size: 1982 4553 BstYI r'GATC_y Cuts at: 1972 2020 2767 3068 3865 4232 4617 4634 6086 Size: 48 747 301 797 367 385 17 1452 Cuts at: 6086 6172 6184 6530 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 797 747 385 367 346 301 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 2288 3572 2288 Size: 1284 5251 BtgI C'CryG_G Cuts at: 513 1065 2004 2559 2649 3111 513 Size: 552 939 555 90 462 3937 Fragments arranged by size: 3937 939 555 552 462 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5796 Fragments arranged by size: 5796 683 56 BtsI GCAGTG_nn' Cuts at: 2669 2700 4051 4845 4865 2669 Size: 31 1351 794 20 4339 Fragments arranged by size: 4339 1351 794 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6535 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6500 DraI TTT'AAA Cuts at: 3839 3839 Size: 6535 DraIII CAC_nnn'GTG Cuts at: 2263 2319 2263 Size: 56 6479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2336 2705 5542 809 Size: 507 1020 369 2837 1802 Fragments arranged by size: 2837 1802 1020 507 369 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3249 3488 3982 Size: 54 1425 50 275 18 1419 239 494 Cuts at: 3982 4871 6500 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1419 889 494 275 239 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3982 1537 Size: 275 2170 4090 Fragments arranged by size: 4090 2170 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4465 1353 Size: 3112 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3329 5122 5496 5642 387 Size: 1064 138 1740 1793 374 146 1280 Fragments arranged by size: 1793 1740 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3355 3355 Size: 6535 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2373 2415 2865 2946 3114 3578 Size: 393 413 328 42 450 81 168 464 Cuts at: 3578 3598 3758 4598 5195 5982 6250 1239 Size: 20 160 840 597 787 268 1524 Fragments arranged by size: 1524 840 787 597 464 450 413 393 328 268 168 160 81 42 20 EcoO109I rG'GnC_Cy Cuts at: 2065 2295 2654 3676 2065 Size: 230 359 1022 4924 Fragments arranged by size: 4924 1022 359 230 EcoRI G'AATT_C Cuts at: 3844 4222 3844 Size: 378 6157 FspI TGC'GCA Cuts at: 5041 5041 Size: 6535 HaeII r_GCGC'y Cuts at: 1678 2238 5682 1678 Size: 560 3444 2531 Fragments arranged by size: 3444 2531 560 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3056 3088 3222 3229 3254 Size: 32 135 32 1484 32 134 7 25 Cuts at: 3254 3261 3416 3448 3743 3775 5176 5208 5250 Size: 7 155 32 295 32 1401 32 42 Cuts at: 5250 5282 1373 Size: 32 2626 Fragments arranged by size: 2626 1484 1401 295 155 135 134 42 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2710 3446 3660 3879 4125 669 Size: 988 281 772 736 214 219 246 3079 Fragments arranged by size: 3079 988 772 736 281 246 219 214 HindIII A'AGCT_T Cuts at: 748 748 Size: 6535 HpaI GTT'AAC Cuts at: 3660 4125 3660 Size: 465 6070 KasI G'GCGC_C Cuts at: 2234 2234 Size: 6535 KpnI G_GTAC'C Cuts at: 3860 3860 Size: 6535 MfeI C'AATT_G Cuts at: 4134 4134 Size: 6535 MluI A'CGCG_T Cuts at: 6411 6411 Size: 6535 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2395 2414 5649 5833 1305 Size: 1090 19 3235 184 2007 Fragments arranged by size: 3235 2007 1090 184 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 3251 10 Size: 54 1425 343 1419 3294 Fragments arranged by size: 3294 1425 1419 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4493 4852 5011 518 Size: 648 3327 359 159 2042 Fragments arranged by size: 3327 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3163 3178 3291 3637 3740 3951 4628 5776 Size: 1557 15 113 346 103 211 677 1148 Cuts at: 5776 6021 1606 Size: 245 2120 Fragments arranged by size: 2120 1557 1148 677 346 245 211 113 103 15 NarI GG'CG_CC Cuts at: 2235 2235 Size: 6535 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5044 Fragments arranged by size: 5044 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6535 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6535 NotI GC'GGCC_GC Cuts at: 3982 3982 Size: 6535 NsiI A_TGCA'T Cuts at: 2984 3757 2984 Size: 773 5762 NspI r_CATG'y Cuts at: 1132 1229 3759 3893 5438 1132 Size: 97 2530 134 1545 2229 Fragments arranged by size: 2530 2229 1545 134 97 PasI CC'CwG_GG Cuts at: 1348 3076 1348 Size: 1728 4807 PciI A'CATG_T Cuts at: 5434 5434 Size: 6535 PflMI CCAn_nnn'nTGG Cuts at: 1108 3807 1108 Size: 2699 3836 PmeI GTTT'AAAC Cuts at: 3839 3839 Size: 6535 PmlI CAC'GTG Cuts at: 2593 3255 2593 Size: 662 5873 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4604 4636 6117 6149 4604 Size: 32 1481 32 4990 Fragments arranged by size: 4990 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2295 2065 Size: 230 6305 PshAI GACnn'nnGTC Cuts at: 1613 3095 3559 3824 1613 Size: 1482 464 265 4324 Fragments arranged by size: 4324 1482 464 265 PsiI TTA'TAA Cuts at: 4105 4105 Size: 6535 PspOMI G'GGCC_C Cuts at: 2653 2653 Size: 6535 PstI C_TGCA'G Cuts at: 830 2136 2255 3183 3887 830 Size: 1306 119 928 704 3478 Fragments arranged by size: 3478 1306 928 704 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6535 PvuII CAG'CTG Cuts at: 3163 3178 3637 3740 3163 Size: 15 459 103 5958 Fragments arranged by size: 5958 459 103 15 SacI G_AGCT'C Cuts at: 3357 3357 Size: 6535 SalI G'TCGA_C Cuts at: 1936 3877 1936 Size: 1941 4594 SanDI GG'GwC_CC Cuts at: 2295 2295 Size: 6535 SbfI CC_TGCA'GG Cuts at: 3887 3887 Size: 6535 ScaI AGT'ACT Cuts at: 4783 6495 4783 Size: 1712 4823 SfcI C'TryA_G Cuts at: 826 1045 2132 2251 3179 3883 5018 5699 5890 Size: 219 1087 119 928 704 1135 681 191 Cuts at: 5890 826 Size: 1471 Fragments arranged by size: 1471 1135 1087 928 704 681 219 191 119 SfoI GGC'GCC Cuts at: 2236 2236 Size: 6535 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6535 SmaI CCC'GGG Cuts at: 1872 3862 1872 Size: 1990 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 3411 4639 5540 5802 820 Size: 197 926 90 1378 1228 901 262 1553 Fragments arranged by size: 1553 1378 1228 926 901 262 197 90 SnaBI TAC'GTA Cuts at: 493 4326 493 Size: 3833 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6535 SphI G_CATG'C Cuts at: 1132 3759 3893 1132 Size: 2627 134 3774 Fragments arranged by size: 3774 2627 134 SspI AAT'ATT Cuts at: 5 52 4459 5 Size: 47 4407 2081 Fragments arranged by size: 4407 2081 47 StuI AGG'CCT Cuts at: 1517 2990 1517 Size: 1473 5062 StyI C'CwwG_G Cuts at: 513 1065 2004 3309 3974 513 Size: 552 939 1305 665 3074 Fragments arranged by size: 3074 1305 939 665 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2842 4562 4579 4732 4917 5362 325 Size: 1255 1262 1720 17 153 185 445 1498 Fragments arranged by size: 1720 1498 1262 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2888 4781 6493 Size: 274 80 33 51 1443 911 1893 1712 Cuts at: 6493 96 Size: 138 Fragments arranged by size: 1893 1712 1443 911 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2716 2748 3270 3302 6495 6527 2716 Size: 32 522 32 3193 32 2724 Fragments arranged by size: 3193 2724 522 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6535 XbaI T'CTAG_A Cuts at: 3871 3871 Size: 6535 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2665 2665 Size: 6535 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6535 XmaI C'CCGG_G Cuts at: 1870 3860 1870 Size: 1990 4545 XmnI GAAnn'nnTTC Cuts at: 4664 4664 Size: 6535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4343 276 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI