(Linear) (Six-base) MAPSORT of: KIEE6783.seq Check: 5853 from: 1 to: 1830 KIEE6783.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 134 192 1830 Size: 134 58 1638 Fragments arranged by size: 1638 134 58 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1573 1593 1830 Size: 1573 20 237 Fragments arranged by size: 1573 237 20 AlwNI CAG_nnn'CTG Cuts at: 0 63 207 441 881 1830 Size: 63 144 234 440 949 Fragments arranged by size: 949 440 234 144 63 ApaI G_GGCC'C Cuts at: 0 652 1830 Size: 652 1178 ApaLI G'TGCA_C Cuts at: 0 306 728 1830 Size: 306 422 1102 Fragments arranged by size: 1102 422 306 ApoI r'AATT_y Cuts at: 0 379 449 523 561 931 1470 1830 Size: 379 70 74 38 370 539 360 Fragments arranged by size: 539 379 370 360 74 70 38 BamHI G'GATC_C Cuts at: 0 762 1830 Size: 762 1068 BanI G'GyrC_C Cuts at: 0 229 819 1492 1830 Size: 229 590 673 338 Fragments arranged by size: 673 590 338 229 BanII G_rGCy'C Cuts at: 0 652 1352 1830 Size: 652 700 478 Fragments arranged by size: 700 652 478 BbeI G_GCGC'C Cuts at: 0 233 1830 Size: 233 1597 BbsI GAAGACnn'nnnn_ Cuts at: 0 1048 1268 1328 1599 1830 Size: 1048 220 60 271 231 Fragments arranged by size: 1048 271 231 220 60 BbvCI CC'TCA_GC Cuts at: 0 1633 1830 Size: 1633 197 BclI T'GATC_A Cuts at: 0 1232 1830 Size: 1232 598 BfrBI ATG'CAT Cuts at: 0 977 1750 1830 Size: 977 773 80 Fragments arranged by size: 977 773 80 BglII A'GATC_T Cuts at: 0 15 1830 Size: 15 1815 BlpI GC'TnA_GC Cuts at: 0 442 1364 1628 1830 Size: 442 922 264 202 Fragments arranged by size: 922 442 264 202 Bme1580I G_kGCm'C Cuts at: 0 267 310 652 732 1201 1830 Size: 267 43 342 80 469 629 Fragments arranged by size: 629 469 342 267 80 43 BmrI ACTGGGnnnn_n' Cuts at: 0 174 261 818 1581 1830 Size: 174 87 557 763 249 Fragments arranged by size: 763 557 249 174 87 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 436 1830 Size: 436 1394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 368 410 860 941 1109 1753 1830 Size: 40 328 42 450 81 168 644 77 Fragments arranged by size: 644 450 328 168 81 77 42 40 Bpu10I CC'TnA_GC Cuts at: 0 1633 1830 Size: 1633 197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 1391 1830 Size: 13 1378 439 Fragments arranged by size: 1378 439 13 BsaI GGTCTCn'nnnn_ Cuts at: 0 65 1120 1185 1830 Size: 65 1055 65 645 Fragments arranged by size: 1055 645 65 65 BsaAI yAC'GTr Cuts at: 0 588 1250 1830 Size: 588 662 580 Fragments arranged by size: 662 588 580 BsaHI Gr'CG_yC Cuts at: 0 230 1830 Size: 230 1600 BsaWI w'CCGG_w Cuts at: 0 137 1830 Size: 137 1693 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 459 489 788 818 1051 1081 1830 Size: 459 30 299 30 233 30 749 Fragments arranged by size: 749 459 299 233 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 109 112 1830 Size: 109 3 1718 Fragments arranged by size: 1718 109 3 BseYI C'CCAG_C Cuts at: 0 207 1154 1623 1830 Size: 207 947 469 207 Fragments arranged by size: 947 469 207 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 516 1810 1830 Size: 205 311 1294 20 Fragments arranged by size: 1294 311 205 20 BsiEI CG_ry'CG Cuts at: 0 1692 1830 Size: 1692 138 BsiHKAI G_wGCw'C Cuts at: 0 27 173 310 732 1352 1830 Size: 27 146 137 422 620 478 Fragments arranged by size: 620 478 422 146 137 27 BsmBI CGTCTCn'nnnn_ Cuts at: 0 672 1830 Size: 672 1158 Bsp1286I G_dGCh'C Cuts at: 0 27 173 267 310 652 732 1201 1352 Size: 27 146 94 43 342 80 469 151 Cuts at: 1352 1830 Size: 478 Fragments arranged by size: 478 469 342 151 146 94 80 43 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 134 192 503 1167 1830 Size: 134 58 311 664 663 Fragments arranged by size: 664 663 311 134 58 BsrBI CCG'CTC Cuts at: 0 1296 1830 Size: 1296 534 BsrGI T'GTAC_A Cuts at: 0 883 1830 Size: 883 947 BssHII G'CGCG_C Cuts at: 0 190 1830 Size: 190 1640 BstAPI GCAn_nnn'nTGC Cuts at: 0 314 423 1830 Size: 314 109 1407 Fragments arranged by size: 1407 314 109 BstEII G'GTnAC_C Cuts at: 0 1161 1830 Size: 1161 669 BstYI r'GATC_y Cuts at: 0 15 762 1063 1830 Size: 15 747 301 767 Fragments arranged by size: 767 747 301 15 Bsu36I CC'TnA_GG Cuts at: 0 283 1567 1830 Size: 283 1284 263 Fragments arranged by size: 1284 283 263 BtgI C'CryG_G Cuts at: 0 554 644 1106 1830 Size: 554 90 462 724 Fragments arranged by size: 724 554 462 90 BtsI GCAGTG_nn' Cuts at: 0 664 695 1830 Size: 664 31 1135 Fragments arranged by size: 1135 664 31 DraIII CAC_nnn'GTG Cuts at: 0 258 314 1830 Size: 258 56 1516 Fragments arranged by size: 1516 258 56 DrdI GACnn_nn'nnGTC Cuts at: 0 331 700 1830 Size: 331 369 1130 Fragments arranged by size: 1130 369 331 EaeI y'GGCC_r Cuts at: 0 1244 1483 1830 Size: 1244 239 347 Fragments arranged by size: 1244 347 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1324 1830 Size: 1324 506 EcoICRI GAG'CTC Cuts at: 0 1350 1830 Size: 1350 480 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 368 410 860 941 1109 1573 1593 Size: 40 328 42 450 81 168 464 20 Cuts at: 1593 1753 1830 Size: 160 77 Fragments arranged by size: 464 450 328 168 160 81 77 42 40 20 EcoO109I rG'GnC_Cy Cuts at: 0 60 290 649 1671 1830 Size: 60 230 359 1022 159 Fragments arranged by size: 1022 359 230 159 60 HaeII r_GCGC'y Cuts at: 0 233 1830 Size: 233 1597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1051 1083 1217 1224 1249 1256 1411 1443 Size: 1051 32 134 7 25 7 155 32 Cuts at: 1443 1738 1770 1830 Size: 295 32 60 Fragments arranged by size: 1051 295 155 134 60 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 0 705 1441 1655 1830 Size: 705 736 214 175 Fragments arranged by size: 736 705 214 175 HpaI GTT'AAC Cuts at: 0 1655 1830 Size: 1655 175 KasI G'GCGC_C Cuts at: 0 229 1830 Size: 229 1601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 390 409 1830 Size: 390 19 1421 Fragments arranged by size: 1421 390 19 MscI TGG'CCA Cuts at: 0 1246 1830 Size: 1246 584 MspA1I CmG'CkG Cuts at: 0 1158 1173 1286 1632 1735 1830 Size: 1158 15 113 346 103 95 Fragments arranged by size: 1158 346 113 103 95 15 NarI GG'CG_CC Cuts at: 0 230 1830 Size: 230 1600 NsiI A_TGCA'T Cuts at: 0 979 1752 1830 Size: 979 773 78 Fragments arranged by size: 979 773 78 NspI r_CATG'y Cuts at: 0 1754 1830 Size: 1754 76 PasI CC'CwG_GG Cuts at: 0 1071 1830 Size: 1071 759 PflMI CCAn_nnn'nTGG Cuts at: 0 1802 1830 Size: 1802 28 PmlI CAC'GTG Cuts at: 0 588 1250 1830 Size: 588 662 580 Fragments arranged by size: 662 588 580 PpuMI rG'GwC_Cy Cuts at: 0 60 290 1830 Size: 60 230 1540 Fragments arranged by size: 1540 230 60 PshAI GACnn'nnGTC Cuts at: 0 1090 1554 1819 1830 Size: 1090 464 265 11 Fragments arranged by size: 1090 464 265 11 PspOMI G'GGCC_C Cuts at: 0 648 1830 Size: 648 1182 PstI C_TGCA'G Cuts at: 0 131 250 1178 1830 Size: 131 119 928 652 Fragments arranged by size: 928 652 131 119 PvuII CAG'CTG Cuts at: 0 1158 1173 1632 1735 1830 Size: 1158 15 459 103 95 Fragments arranged by size: 1158 459 103 95 15 SacI G_AGCT'C Cuts at: 0 1352 1830 Size: 1352 478 SanDI GG'GwC_CC Cuts at: 0 290 1830 Size: 290 1540 SfcI C'TryA_G Cuts at: 0 127 246 1174 1830 Size: 127 119 928 656 Fragments arranged by size: 928 656 127 119 SfoI GGC'GCC Cuts at: 0 231 1830 Size: 231 1599 SmlI C'TyrA_G Cuts at: 0 28 1406 1830 Size: 28 1378 424 Fragments arranged by size: 1378 424 28 SphI G_CATG'C Cuts at: 0 1754 1830 Size: 1754 76 StuI AGG'CCT Cuts at: 0 985 1830 Size: 985 845 StyI C'CwwG_G Cuts at: 0 1304 1830 Size: 1304 526 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 837 1830 Size: 837 993 TatI w'GTAC_w Cuts at: 0 883 1830 Size: 883 947 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 711 743 1265 1297 1830 Size: 711 32 522 32 533 Fragments arranged by size: 711 533 522 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 660 1830 Size: 660 1170 Enzymes that do cut: AarI AcuI AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbeI BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrGI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HpaI KasI MmeI MscI MspA1I NarI NsiI NspI PasI PflMI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BcgI BciVI BglI BmgBI BmtI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrFI BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI