(Circular) (Six-base) MAPSORT of: pFN21AE5778.seq Check: 545 from: 1 to: 6580 pFN21AE5778 6580 bp With 182 enzymes: SgfI * June 20, 2019 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2096 3199 2096 Size: 1103 5477 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2436 4390 278 Size: 53 83 186 936 900 1954 2468 Fragments arranged by size: 2468 1954 936 900 186 83 53 AccI GT'mk_AC Cuts at: 1937 2500 2842 3336 3923 1937 Size: 563 342 494 587 4594 Fragments arranged by size: 4594 587 563 494 342 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2525 4643 6027 6295 1632 Size: 399 494 2118 1384 268 1917 Fragments arranged by size: 2118 1917 1384 494 399 268 AfeI AGC'GCT Cuts at: 2329 2329 Size: 6580 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 5479 6456 1203 Size: 4276 977 1327 Fragments arranged by size: 4276 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 3249 3833 1166 Size: 2083 584 3913 Fragments arranged by size: 3913 2083 584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AlwNI CAG_nnn'CTG Cuts at: 2054 2962 4323 5895 2054 Size: 908 1361 1572 2739 Fragments arranged by size: 2739 1572 1361 908 ApaLI G'TGCA_C Cuts at: 2583 2965 4637 5793 2583 Size: 382 1672 1156 3370 Fragments arranged by size: 3370 1672 1156 382 ApoI r'AATT_y Cuts at: 1493 3889 4115 4267 6376 1493 Size: 2396 226 152 2109 1697 Fragments arranged by size: 2396 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 3905 1870 Size: 73 1962 4545 Fragments arranged by size: 4545 1962 73 BamHI G'GATC_C Cuts at: 2209 3910 4277 2209 Size: 1701 367 4512 Fragments arranged by size: 4512 1701 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3901 5356 618 Size: 325 217 627 2114 1455 1842 Fragments arranged by size: 2114 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3899 1391 Size: 2508 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 928 Size: 971 211 5398 Fragments arranged by size: 5398 971 211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3483 3517 3782 3816 4771 4805 1903 Size: 34 1546 34 265 34 955 34 3678 Fragments arranged by size: 3678 1546 955 265 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3101 4472 5682 3101 Size: 1371 1210 3999 Fragments arranged by size: 3999 1371 1210 BclI T'GATC_A Cuts at: 1548 3412 1548 Size: 1864 4716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6575 6575 Size: 6580 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 2587 2969 4641 5797 1790 Size: 797 382 1672 1156 2573 Fragments arranged by size: 2573 1672 1156 797 382 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2793 2861 4311 Size: 416 53 83 399 24 1376 68 1450 Cuts at: 4311 5269 442 Size: 958 1753 Fragments arranged by size: 1753 1450 1376 958 416 399 83 68 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2841 2841 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 3584 3881 5240 1239 Size: 1262 1083 297 1359 2579 Fragments arranged by size: 2579 1359 1262 1083 297 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2388 3855 4705 5570 5868 2388 Size: 1467 850 865 298 3100 Fragments arranged by size: 3100 1467 865 850 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5243 882 Size: 617 3744 2219 Fragments arranged by size: 3744 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2454 3847 4371 493 Size: 711 1250 1393 524 2702 Fragments arranged by size: 2702 1393 1250 711 524 BsaBI GATnn'nnATC Cuts at: 6574 6574 Size: 6580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2433 4387 4769 5404 Size: 53 83 186 936 900 1954 382 635 Cuts at: 5404 275 Size: 1451 Fragments arranged by size: 1954 1451 936 900 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5013 5411 5685 5832 6243 5013 Size: 398 274 147 411 5350 Fragments arranged by size: 5350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6355 Fragments arranged by size: 6355 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3108 1186 Size: 1922 4658 BseYI C'CCAG_C Cuts at: 2474 3004 3546 3786 5783 6279 2474 Size: 530 542 240 1997 496 2775 Fragments arranged by size: 2775 1997 542 530 496 240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 2677 3203 1163 Size: 573 401 540 526 4540 Fragments arranged by size: 4540 573 540 526 401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2404 2587 2969 3805 4641 4726 5797 2404 Size: 183 382 836 836 85 1071 3187 Fragments arranged by size: 3187 1071 836 836 382 183 85 BsiWI C'GTAC_G Cuts at: 2454 2454 Size: 6580 BsmI GAATG_Cn' Cuts at: 2414 4091 4184 2414 Size: 1677 93 4810 Fragments arranged by size: 4810 1677 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2404 2587 2969 3805 3899 4641 4726 Size: 399 614 183 382 836 94 742 85 Cuts at: 4726 5797 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 836 742 614 399 382 183 94 85 BspEI T'CCGG_A Cuts at: 6243 6243 Size: 6580 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2096 3199 3935 844 Size: 1252 1103 736 3489 Fragments arranged by size: 3489 1252 1103 736 BsrBI CCG'CTC Cuts at: 1394 1823 3293 3814 4467 1394 Size: 429 1470 521 653 3507 Fragments arranged by size: 3507 1470 653 521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2315 5075 5249 65 Size: 1183 1067 2760 174 1396 Fragments arranged by size: 2760 1396 1183 1067 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3686 5224 5411 1700 Size: 110 1876 1538 187 2869 Fragments arranged by size: 2869 1876 1538 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 2945 3781 4640 5652 2945 Size: 836 859 1012 3873 Fragments arranged by size: 3873 1012 859 836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6580 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 3211 1184 Size: 2027 4553 BstYI r'GATC_y Cuts at: 1972 2209 3065 3910 4277 4662 4679 6131 6217 Size: 237 856 845 367 385 17 1452 86 Cuts at: 6217 6229 6575 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 856 845 385 367 346 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3203 513 Size: 552 2138 3890 Fragments arranged by size: 3890 2138 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 2258 2437 3882 4096 4890 4910 2258 Size: 179 1445 214 794 20 3928 Fragments arranged by size: 3928 1445 794 214 179 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 3351 3884 3351 Size: 533 6047 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2683 3200 3815 Size: 54 1425 50 275 18 853 517 615 Cuts at: 3815 4027 4916 6545 8 Size: 212 889 1629 43 Fragments arranged by size: 1629 1425 889 853 615 517 275 212 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 3627 3638 4510 1353 Size: 830 1444 11 872 3423 Fragments arranged by size: 3423 1444 872 830 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2207 5167 5541 5687 387 Size: 1064 138 618 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 618 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2501 2525 3584 3881 4643 5240 Size: 393 399 470 24 1059 297 762 597 Cuts at: 5240 6027 6295 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1059 787 762 597 470 399 393 297 268 24 EcoNI CCTnn'n_nnAGG Cuts at: 3513 3513 Size: 6580 EcoO109I rG'GnC_Cy Cuts at: 3734 3734 Size: 6580 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3061 3634 3061 Size: 573 6007 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 2133 2331 5727 1678 Size: 455 198 3396 2531 Fragments arranged by size: 3396 2531 455 198 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2160 2192 3084 3116 3246 Size: 32 135 32 588 32 892 32 130 Cuts at: 3246 3278 3362 3394 5221 5253 5295 5327 1373 Size: 32 84 32 1827 32 42 32 2626 Fragments arranged by size: 2626 1827 892 588 135 130 84 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3924 4170 669 Size: 988 281 1986 246 3079 Fragments arranged by size: 3079 1986 988 281 246 HindIII A'AGCT_T Cuts at: 748 3050 748 Size: 2302 4278 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3095 3151 5694 5878 1305 Size: 1790 56 2543 184 2007 Fragments arranged by size: 2543 2007 1790 184 56 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4758 Fragments arranged by size: 4758 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2867 3249 3833 4538 4897 5056 Size: 648 1182 519 382 584 705 359 159 Cuts at: 5056 518 Size: 2042 Fragments arranged by size: 2042 1182 705 648 584 519 382 359 159 MspA1I CmG'CkG Cuts at: 1606 2982 3996 4673 5821 6066 1606 Size: 1376 1014 677 1148 245 2120 Fragments arranged by size: 2120 1376 1148 1014 677 245 NaeI GCC'GGC Cuts at: 3688 3688 Size: 6580 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6028 NdeI CA'TA_TG Cuts at: 387 3500 387 Size: 3113 3467 NgoMIV G'CCGG_C Cuts at: 3686 3686 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 3938 5483 1132 Size: 97 2709 1545 2229 Fragments arranged by size: 2709 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 5479 5479 Size: 6580 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 1108 Size: 1112 5468 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PstI C_TGCA'G Cuts at: 830 2855 3932 830 Size: 2025 1077 3478 Fragments arranged by size: 3478 2025 1077 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 2138 4828 6540 2138 Size: 2690 1712 2178 Fragments arranged by size: 2690 2178 1712 SfcI C'TryA_G Cuts at: 826 1045 2851 3337 3691 3928 5063 5744 5935 Size: 219 1806 486 354 237 1135 681 191 Cuts at: 5935 826 Size: 1471 Fragments arranged by size: 1806 1471 1135 681 486 354 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2403 3870 4684 5585 5847 820 Size: 197 926 460 1467 814 901 262 1553 Fragments arranged by size: 1553 1467 926 901 814 460 262 197 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 2951 1517 Size: 1434 5146 StyI C'CwwG_G Cuts at: 513 1065 4019 513 Size: 552 2954 3074 Fragments arranged by size: 3074 2954 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2178 2528 3247 3538 4607 4624 4777 Size: 1255 598 350 719 291 1069 17 153 Cuts at: 4777 4962 5407 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1069 719 598 445 350 291 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2801 4826 Size: 274 80 33 51 1443 159 665 2025 Cuts at: 4826 6538 96 Size: 1712 138 Fragments arranged by size: 2025 1712 1443 665 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2686 2718 3125 3157 6540 6572 2686 Size: 32 407 32 3383 32 2694 Fragments arranged by size: 3383 2694 407 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2654 1316 Size: 1338 5242 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 4709 4709 Size: 6580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2434 4388 276 Size: 53 83 186 936 900 1954 2468 Fragments arranged by size: 2468 1954 936 900 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI