Restriction map for
pFN21AE5680
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BbeI BcgI BciVI BfaI
BfrI BglII BlnI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeIII MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PmeI PpuMI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1165 GTCTAC GT^[AC][GT]AC
AfaI 547 GTAC GT^AC
AocI 735 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1020 AGCGCT AGC^GCT
Asp700I 660 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 96 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 655 CCCGGG C^[CT]CG[GA]G
AviII 1025 TGCGCA TGC^GCA
BamHI 1325 GGATCC G^GATCC
BbrPI 1103 CACGTG CAC^GTG
BbsI 664 GTCTTC GAAGAC(2/6)
BclI 185 TGATCA T^GATCA
BglI 1339 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 243 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 243 GCTGAGC GC^T[ACGT]AGC
BpuAI 664 GTCTTC GAAGAC(2/6)
BsiEI 383 CGGCCG CG[GA][CT]^CG
BsmFI 1265 GGGAC GGGAC(10/14)
BsmI 760 GAATGC GAATGC(1/-1)
BsoBI 655 CCCGGG C^[CT]CG[GA]G
BspHI 1499 TCATGA T^CATGA
BssHII 416 GCGCGC G^CGCGC
Bsu36I 735 CCTCAGG CC^T[ACGT]AGG
CelII 243 GCTGAGC GC^T[ACGT]AGC
DraIII 1279 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 383 CGGCCG C^GGCCG
EclXI 383 CGGCCG C^GGCCG
Eco47III 1020 AGCGCT AGC^GCT
Eco52I 383 CGGCCG C^GGCCG
Eco81I 735 CCTCAGG CC^T[ACGT]AGG
FbaI 185 TGATCA T^GATCA
FspI 1025 TGCGCA TGC^GCA
MspA1I 241 CCGCTG C[AC]G^C[GT]G
NcoI 89 CCATGG C^CATGG
PflFI 96 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1103 CACGTG CAC^GTG
PmlI 1103 CACGTG CAC^GTG
PshAI 856 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1499 TCATGA T^CATGA
RsaI 547 GTAC GT^AC
SfiI 1338 GGCCTTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 655 CCCGGG CCC^GGG
SmlI 1390 CTCAAG C^T[CT][GA]AG
Tth111I 96 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 655 CCCGGG C^CCGGG
XmaI 655 CCCGGG C^CCGGG
XmnI 660 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 96 GACGCC G[GA]^CG[CT]C
151 GACGCC
AflIII 920 ACGTGT A^C[GA][CT]GT
1102 ACACGT
AgeI 233 ACCGGT A^CCGGT
1441 ACCGGT
ApaI 877 GGGCCC GGGCC^C
1530 GGGCCC
BanI 142 GGTGCC G^G[CT][GA]CC
1272 GGTGCC
BbvCI 1305 CCTCAGC CCTCAGC(-5/-2)
495 GCTGAGG
BsaBI 180 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATCCTCATC
BsaHI 96 GACGCC G[GA]^CG[CT]C
151 GACGCC
BsaI 565 GAGACC GGTCTC(1/5)
796 GAGACC
BsgI 532 CTGCAC GTGCAG(16/14)
292 GTGCAG
BsmBI 17 GAGACG CGTCTC(1/5)
94 GAGACG
BsrBI 969 GAGCGG CCGCTC(-3/-3)
1320 GAGCGG
BstYI 179 AGATCC [GA]^GATC[CT]
1325 GGATCC
DsaI 89 CCATGG C^C[GA][CT]GG
1136 CCGTGG
EcoNI 168 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
984 CCTGGAGAAGG
EcoT14I 64 CCTTGG C^C[AT][AT]GG
89 CCATGG
HaeII 352 AGCGCC [GA]GCGC^[CT]
1020 AGCGCT
Hin1I 96 GACGCC G[GA]^CG[CT]C
151 GACGCC
HinfI 69 GAGTC G^A[ACGT]TC
1183 GAGTC
MamI 180 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATCCTCATC
MflI 179 AGATCC [GA]^GATC[CT]
1325 GGATCC
PflMI 1056 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1293 CCAGATGGTGG
PinAI 233 ACCGGT A^CCGGT
1441 ACCGGT
PleI 69 GAGTC GAGTC(4/5)
1183 GAGTC
PstI 403 CTGCAG CTGCA^G
1069 CTGCAG
SfcI 403 CTGCAG C^T[GA][CT]AG
1069 CTGCAG
StyI 64 CCTTGG C^C[AT][AT]GG
89 CCATGG
Van91I 1056 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1293 CCAGATGGTGG
XcmI 680 CCATCCTCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1244 CCATGACAACGGTGG
XhoII 179 AGATCC [GA]^GATC[CT]
1325 GGATCC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV SacI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I