(Circular) (Six-base) MAPSORT of: pFN21AE5680.seq Check: 5012 from: 1 to: 6262 pFN21AE5680 6262 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3171 3605 1937 Size: 1234 434 4594 Fragments arranged by size: 4594 1234 434 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 3401 4325 5709 5977 1632 Size: 563 1206 924 1384 268 1917 Fragments arranged by size: 1917 1384 1206 924 563 268 AfeI AGC'GCT Cuts at: 3027 3027 Size: 6262 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 2925 3107 5161 6138 1203 Size: 1722 182 2054 977 1327 Fragments arranged by size: 2054 1722 1327 977 182 AgeI A'CCGG_T Cuts at: 2238 3446 5093 2238 Size: 1208 1647 3407 Fragments arranged by size: 3407 1647 1208 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AlwNI CAG_nnn'CTG Cuts at: 2083 2274 3082 4005 5577 2083 Size: 191 808 923 1572 2768 Fragments arranged by size: 2768 1572 923 808 191 ApaI G_GGCC'C Cuts at: 2886 3539 2886 Size: 653 5609 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 3571 3797 3949 6058 1493 Size: 2078 226 152 2109 1697 Fragments arranged by size: 2109 2078 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2660 3587 1870 Size: 73 717 927 4545 Fragments arranged by size: 4545 927 717 73 BamHI G'GATC_C Cuts at: 3330 3592 3959 3330 Size: 262 367 5633 Fragments arranged by size: 5633 367 262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 3277 3583 5038 618 Size: 325 217 627 360 1130 306 1455 1842 Fragments arranged by size: 1842 1455 1130 627 360 325 306 217 BanII G_rGCy'C Cuts at: 1391 2354 2886 2952 2984 3090 3396 3504 3539 Size: 963 532 66 32 106 306 108 35 Cuts at: 3539 3581 1391 Size: 42 4072 Fragments arranged by size: 4072 963 532 306 108 106 66 42 35 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2662 928 Size: 971 763 4528 Fragments arranged by size: 4528 971 763 BbvCI CC'TCA_GC Cuts at: 2501 3311 2501 Size: 810 5452 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4453 4487 1903 Size: 34 50 34 2432 34 3678 Fragments arranged by size: 3678 2432 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 2190 1548 Size: 642 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3350 4873 136 Size: 107 122 71 631 605 1678 1523 1525 Fragments arranged by size: 1678 1525 1523 631 605 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 2249 3679 2249 Size: 1430 4832 Bme1580I G_kGCm'C Cuts at: 1790 2152 2886 3282 3539 4323 5479 1790 Size: 362 734 396 257 784 1156 2573 Fragments arranged by size: 2573 1156 784 734 396 362 257 BmgBI CAC'GTC Cuts at: 1318 3357 1318 Size: 2039 4223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2159 2461 3993 Size: 416 53 83 399 24 742 302 1532 Cuts at: 3993 4951 442 Size: 958 1753 Fragments arranged by size: 1753 1532 958 742 416 399 302 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2245 2495 2531 2540 2849 3011 3224 3410 Size: 1006 250 36 9 309 162 213 186 Cuts at: 3410 3551 4922 1239 Size: 141 1371 2579 Fragments arranged by size: 2579 1371 1006 309 250 213 186 162 141 36 9 Bpu10I CC'TnA_GC Cuts at: 2258 2501 3311 3323 2258 Size: 243 810 12 5197 Fragments arranged by size: 5197 810 243 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3380 4387 5252 5550 3380 Size: 1007 865 298 4092 Fragments arranged by size: 4092 1007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 2795 4925 882 Size: 617 1065 231 2130 2219 Fragments arranged by size: 2219 2130 1065 617 231 BsaAI yAC'GTr Cuts at: 493 1204 2926 3043 3110 3175 4053 493 Size: 711 1722 117 67 65 878 2702 Fragments arranged by size: 2702 1722 878 711 117 67 65 BsaBI GATnn'nnATC Cuts at: 2189 3335 6256 2189 Size: 1146 2921 2195 Fragments arranged by size: 2921 2195 1146 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 2157 4069 4451 Size: 53 83 186 936 569 55 1912 382 Cuts at: 4451 5086 275 Size: 635 1451 Fragments arranged by size: 1912 1451 936 635 569 382 186 83 55 53 BsaWI w'CCGG_w Cuts at: 2127 2238 3446 4695 5093 5367 5514 5925 2127 Size: 111 1208 1249 398 274 147 411 2464 Fragments arranged by size: 2464 1249 1208 411 398 274 147 111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 3359 3389 1178 Size: 30 165 30 687 30 1239 30 4051 Fragments arranged by size: 4051 1239 687 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2303 2453 2477 2528 2537 2584 2765 Size: 1021 96 150 24 51 9 47 181 Cuts at: 2765 2960 1186 Size: 195 4488 Fragments arranged by size: 4488 1021 195 181 150 96 51 47 24 9 BseYI C'CCAG_C Cuts at: 2354 2601 3067 3082 3538 5465 5961 2354 Size: 247 466 15 456 1927 496 2655 Fragments arranged by size: 2655 1927 496 466 456 247 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2522 1163 Size: 573 582 204 4903 Fragments arranged by size: 4903 582 573 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2391 3712 4473 5501 1540 Size: 275 186 390 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 390 275 186 BsiHKAI G_wGCw'C Cuts at: 2231 2984 3396 3504 4323 4408 5479 2231 Size: 753 412 108 819 85 1071 3014 Fragments arranged by size: 3014 1071 819 753 412 108 85 BsmI GAATG_Cn' Cuts at: 2771 3773 3866 2771 Size: 1002 93 5167 Fragments arranged by size: 5167 1002 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2093 2016 Size: 77 6185 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2231 2354 2886 2952 2984 3090 Size: 399 362 79 123 532 66 32 106 Cuts at: 3090 3282 3396 3504 3539 3581 4323 4408 5479 Size: 192 114 108 35 42 742 85 1071 Cuts at: 5479 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 532 399 362 192 123 114 108 106 85 79 66 42 35 32 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 3504 4151 5881 3504 Size: 647 1730 3885 Fragments arranged by size: 3885 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3617 844 Size: 2773 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2976 3327 4149 1394 Size: 429 1153 351 822 3507 Fragments arranged by size: 3507 1153 822 429 351 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2238 2619 3446 4906 5093 1700 Size: 110 428 381 827 1460 187 2869 Fragments arranged by size: 2869 1460 827 428 381 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 2421 1925 Size: 496 5766 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 2054 2095 2812 2054 Size: 41 717 5504 Fragments arranged by size: 5504 717 41 BstYI r'GATC_y Cuts at: 1972 2184 3330 3592 3959 4344 4361 5813 5899 Size: 212 1146 262 367 385 17 1452 86 Cuts at: 5899 5911 6257 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1146 385 367 346 262 212 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2741 2741 Size: 6262 BtgI C'CryG_G Cuts at: 513 1065 2094 3141 513 Size: 552 1029 1047 3634 Fragments arranged by size: 3634 1047 1029 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2322 487 Size: 683 56 1096 4427 Fragments arranged by size: 4427 1096 683 56 BtsI GCAGTG_nn' Cuts at: 3778 4572 4592 3778 Size: 794 20 5448 Fragments arranged by size: 5448 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2051 2086 529 Size: 35 1487 35 4705 Fragments arranged by size: 4705 1487 35 35 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DraIII CAC_nnn'GTG Cuts at: 3289 3289 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 2529 2697 Size: 54 1425 50 275 18 558 141 168 Cuts at: 2697 2907 3138 3144 3177 3351 3486 3709 4598 Size: 210 231 6 33 174 135 223 889 Cuts at: 4598 6227 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 558 275 231 223 210 174 168 141 135 54 50 43 33 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2388 3709 1537 Size: 275 576 1321 4090 Fragments arranged by size: 4090 1321 576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2696 3137 3161 4192 1353 Size: 1343 441 24 1031 3423 Fragments arranged by size: 3423 1343 1031 441 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3419 4849 5223 5369 387 Size: 1064 138 1830 1430 374 146 1280 Fragments arranged by size: 1830 1430 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2982 3394 3502 2982 Size: 412 108 5742 Fragments arranged by size: 5742 412 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2245 2495 2531 2540 2849 3011 Size: 393 563 50 250 36 9 309 162 Cuts at: 3011 3224 3401 3410 3551 4325 4922 5709 5977 Size: 213 177 9 141 774 597 787 268 Cuts at: 5977 1239 Size: 1524 Fragments arranged by size: 1524 787 774 597 563 393 309 268 250 213 177 162 141 50 36 9 9 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2993 2177 Size: 816 5446 EcoO109I rG'GnC_Cy Cuts at: 2029 2116 2605 2882 2883 3535 2029 Size: 87 489 277 1 652 4756 Fragments arranged by size: 4756 652 489 277 87 1 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FspI TGC'GCA Cuts at: 3032 4768 3032 Size: 1736 4526 HaeII r_GCGC'y Cuts at: 1678 2361 3029 5409 1678 Size: 683 668 2380 2531 Fragments arranged by size: 2531 2380 683 668 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 3173 3205 4903 Size: 32 135 32 518 32 1051 32 1698 Cuts at: 4903 4935 4977 5009 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1698 1051 518 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2258 5376 5560 1305 Size: 953 3118 184 2007 Fragments arranged by size: 3118 2007 953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 2699 2909 3146 3488 Size: 54 1425 343 699 168 210 237 342 Cuts at: 3488 10 Size: 2784 Fragments arranged by size: 2784 1425 699 343 342 237 210 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2052 2810 3248 3557 4220 4579 Size: 648 843 43 758 438 309 663 359 Cuts at: 4579 4738 518 Size: 159 2042 Fragments arranged by size: 2042 843 758 663 648 438 359 309 159 43 MspA1I CmG'CkG Cuts at: 1606 2248 3678 4355 5503 5748 1606 Size: 642 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 677 642 245 NcoI C'CATG_G Cuts at: 513 1065 2094 513 Size: 552 1029 4681 Fragments arranged by size: 4681 1029 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 3620 5165 1132 Size: 97 2391 1545 2229 Fragments arranged by size: 2391 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 3067 3304 1108 Size: 1959 237 4066 Fragments arranged by size: 4066 1959 237 PfoI T'CCnGG_A Cuts at: 2433 3471 3528 2433 Size: 1038 57 5167 Fragments arranged by size: 5167 1038 57 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PmlI CAC'GTG Cuts at: 3110 3110 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3486 3518 4331 4363 5844 5876 3486 Size: 32 813 32 1481 32 3872 Fragments arranged by size: 3872 1481 813 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2865 1613 Size: 1252 5010 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 2882 3535 2882 Size: 653 5609 PstI C_TGCA'G Cuts at: 830 2412 3078 3614 830 Size: 1582 666 536 3478 Fragments arranged by size: 3478 1582 666 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 SacI G_AGCT'C Cuts at: 2984 3396 3504 2984 Size: 412 108 5742 Fragments arranged by size: 5742 412 108 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SfcI C'TryA_G Cuts at: 826 1045 2408 3074 3610 4745 5426 5617 826 Size: 219 1363 666 536 1135 681 191 1471 Fragments arranged by size: 1471 1363 1135 681 666 536 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3350 3350 Size: 6262 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 2662 3589 1872 Size: 790 927 4545 Fragments arranged by size: 4545 927 790 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 4366 5267 5529 820 Size: 197 926 1452 971 901 262 1553 Fragments arranged by size: 1553 1452 971 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 2069 2094 3701 513 Size: 552 1004 25 1607 3074 Fragments arranged by size: 3074 1607 1004 552 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 4289 4306 4459 4644 5089 325 Size: 1255 555 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 555 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4508 6220 96 Size: 274 80 33 51 1443 2531 1712 138 Fragments arranged by size: 2531 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 2131 2163 3359 3391 6222 6254 2042 Size: 32 57 32 1196 32 2831 32 2050 Fragments arranged by size: 2831 2050 1196 57 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2104 1316 Size: 788 5474 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2692 3256 2692 Size: 564 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 2660 3587 1870 Size: 790 927 4545 Fragments arranged by size: 4545 927 790 XmnI GAAnn'nnTTC Cuts at: 2669 4391 2669 Size: 1722 4540 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI