Restriction map for
pFN21AE5680
(Linear) (Six-base) MAPSORT of: KIEE5680.seq Check: 7695 from: 1 to: 1557
KIEE5680.orf
With 182 enzymes: SgfI *
December 5, 2014 13:06 ..
AccI GT'mk_AC
Cuts at: 0 1166 1557
Size: 1166 391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1396 1557
Size: 190 1206 161
Fragments arranged by size:
1206 190 161
AfeI AGC'GCT
Cuts at: 0 1022 1557
Size: 1022 535
AflIII A'CryG_T
Cuts at: 0 920 1102 1557
Size: 920 182 455
Fragments arranged by size:
920 455 182
AgeI A'CCGG_T
Cuts at: 0 233 1441 1557
Size: 233 1208 116
Fragments arranged by size:
1208 233 116
AlwNI CAG_nnn'CTG
Cuts at: 0 78 269 1077 1557
Size: 78 191 808 480
Fragments arranged by size:
808 480 191 78
ApaI G_GGCC'C
Cuts at: 0 881 1534 1557
Size: 881 653 23
Fragments arranged by size:
881 653 23
AvaI C'yCGr_G
Cuts at: 0 655 1557
Size: 655 902
BamHI G'GATC_C
Cuts at: 0 1325 1557
Size: 1325 232
BanI G'GyrC_C
Cuts at: 0 142 1272 1557
Size: 142 1130 285
Fragments arranged by size:
1130 285 142
BanII G_rGCy'C
Cuts at: 0 349 881 947 979 1085 1391 1499 1534
Size: 349 532 66 32 106 306 108 35
Cuts at: 1534 1557
Size: 23
Fragments arranged by size:
532 349 306 108 106 66 35 32
23
BbsI GAAGACnn'nnnn_
Cuts at: 0 657 1557
Size: 657 900
BbvCI CC'TCA_GC
Cuts at: 0 496 1306 1557
Size: 496 810 251
Fragments arranged by size:
810 496 251
BclI T'GATC_A
Cuts at: 0 185 1557
Size: 185 1372
BglI GCCn_nnn'nGGC
Cuts at: 0 1345 1557
Size: 1345 212
BlpI GC'TnA_GC
Cuts at: 0 244 1557
Size: 244 1313
Bme1580I G_kGCm'C
Cuts at: 0 147 881 1277 1534 1557
Size: 147 734 396 257 23
Fragments arranged by size:
734 396 257 147 23
BmgBI CAC'GTC
Cuts at: 0 1352 1557
Size: 1352 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 154 456 1557
Size: 154 302 1101
Fragments arranged by size:
1101 302 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 490 526 535 844 1006 1219 1405
Size: 240 250 36 9 309 162 213 186
Cuts at: 1405 1546 1557
Size: 141 11
Fragments arranged by size:
309 250 240 213 186 162 141 36
11 9
Bpu10I CC'TnA_GC
Cuts at: 0 253 496 1306 1318 1557
Size: 253 243 810 12 239
Fragments arranged by size:
810 253 243 239 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1557
Size: 1375 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 790 1557
Size: 559 231 767
Fragments arranged by size:
767 559 231
BsaAI yAC'GTr
Cuts at: 0 921 1038 1105 1170 1557
Size: 921 117 67 65 387
Fragments arranged by size:
921 387 117 67 65
BsaBI GATnn'nnATC
Cuts at: 0 184 1330 1557
Size: 184 1146 227
Fragments arranged by size:
1146 227 184
BsaHI Gr'CG_yC
Cuts at: 0 97 152 1557
Size: 97 55 1405
Fragments arranged by size:
1405 97 55
BsaWI w'CCGG_w
Cuts at: 0 122 233 1441 1557
Size: 122 111 1208 116
Fragments arranged by size:
1208 122 116 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 1354 1384 1557
Size: 85 30 1239 30 173
Fragments arranged by size:
1239 173 85 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 298 448 472 523 532 579 760
Size: 202 96 150 24 51 9 47 181
Cuts at: 760 955 1557
Size: 195 602
Fragments arranged by size:
602 202 195 181 150 96 51 47
24 9
BseYI C'CCAG_C
Cuts at: 0 349 596 1062 1077 1533 1557
Size: 349 247 466 15 456 24
Fragments arranged by size:
466 456 349 247 24 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 517 1557
Size: 313 204 1040
Fragments arranged by size:
1040 313 204
BsiEI CG_ry'CG
Cuts at: 0 386 1557
Size: 386 1171
BsiHKAI G_wGCw'C
Cuts at: 0 226 979 1391 1499 1557
Size: 226 753 412 108 58
Fragments arranged by size:
753 412 226 108 58
BsmI GAATG_Cn'
Cuts at: 0 766 1557
Size: 766 791
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 88 1557
Size: 11 77 1469
Fragments arranged by size:
1469 77 11
Bsp1286I G_dGCh'C
Cuts at: 0 147 226 349 881 947 979 1085 1277
Size: 147 79 123 532 66 32 106 192
Cuts at: 1277 1391 1499 1534 1557
Size: 114 108 35 23
Fragments arranged by size:
532 192 147 123 114 108 106 79
66 35 32 23
BspHI T'CATG_A
Cuts at: 0 1499 1557
Size: 1499 58
BsrBI CCG'CTC
Cuts at: 0 971 1322 1557
Size: 971 351 235
Fragments arranged by size:
971 351 235
BsrFI r'CCGG_y
Cuts at: 0 233 614 1441 1557
Size: 233 381 827 116
Fragments arranged by size:
827 381 233 116
BssHII G'CGCG_C
Cuts at: 0 416 1557
Size: 416 1141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 49 90 807 1557
Size: 49 41 717 750
Fragments arranged by size:
750 717 49 41
BstYI r'GATC_y
Cuts at: 0 179 1325 1557
Size: 179 1146 232
Fragments arranged by size:
1146 232 179
Bsu36I CC'TnA_GG
Cuts at: 0 736 1557
Size: 736 821
BtgI C'CryG_G
Cuts at: 0 89 1136 1557
Size: 89 1047 421
Fragments arranged by size:
1047 421 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 317 1557
Size: 317 1240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 46 81 1557
Size: 46 35 1476
Fragments arranged by size:
1476 46 35
DraIII CAC_nnn'GTG
Cuts at: 0 1284 1557
Size: 1284 273
EaeI y'GGCC_r
Cuts at: 0 383 524 692 902 1133 1139 1172 1346
Size: 383 141 168 210 231 6 33 174
Cuts at: 1346 1481 1557
Size: 135 76
Fragments arranged by size:
383 231 210 174 168 141 135 76
33 6
EagI C'GGCC_G
Cuts at: 0 383 1557
Size: 383 1174
EarI CTCTTCn'nnn_
Cuts at: 0 691 1132 1156 1557
Size: 691 441 24 401
Fragments arranged by size:
691 441 401 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1414 1557
Size: 1414 143
EcoICRI GAG'CTC
Cuts at: 0 977 1389 1497 1557
Size: 977 412 108 60
Fragments arranged by size:
977 412 108 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 240 490 526 535 844 1006 1219
Size: 190 50 250 36 9 309 162 213
Cuts at: 1219 1396 1405 1546 1557
Size: 177 9 141 11
Fragments arranged by size:
309 250 213 190 177 162 141 50
36 11 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 988 1557
Size: 172 816 569
Fragments arranged by size:
816 569 172
EcoO109I rG'GnC_Cy
Cuts at: 0 24 111 600 877 878 1530 1557
Size: 24 87 489 277 1 652 27
Fragments arranged by size:
652 489 277 87 27 24 1
FspI TGC'GCA
Cuts at: 0 1027 1557
Size: 1027 530
HaeII r_GCGC'y
Cuts at: 0 356 1024 1557
Size: 356 668 533
Fragments arranged by size:
668 533 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1168 1200 1557
Size: 85 32 1051 32 357
Fragments arranged by size:
1051 357 85 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1557
Size: 253 1304
MscI TGG'CCA
Cuts at: 0 526 694 904 1141 1483 1557
Size: 526 168 210 237 342 74
Fragments arranged by size:
526 342 237 210 168 74
MslI CAynn'nnrTG
Cuts at: 0 47 805 1243 1552 1557
Size: 47 758 438 309 5
Fragments arranged by size:
758 438 309 47 5
MspA1I CmG'CkG
Cuts at: 0 243 1557
Size: 243 1314
NcoI C'CATG_G
Cuts at: 0 89 1557
Size: 89 1468
PflMI CCAn_nnn'nTGG
Cuts at: 0 1062 1299 1557
Size: 1062 237 258
Fragments arranged by size:
1062 258 237
PfoI T'CCnGG_A
Cuts at: 0 428 1466 1523 1557
Size: 428 1038 57 34
Fragments arranged by size:
1038 428 57 34
PmlI CAC'GTG
Cuts at: 0 1105 1557
Size: 1105 452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1481 1513 1557
Size: 1481 32 44
Fragments arranged by size:
1481 44 32
PshAI GACnn'nnGTC
Cuts at: 0 860 1557
Size: 860 697
PspOMI G'GGCC_C
Cuts at: 0 877 1530 1557
Size: 877 653 27
Fragments arranged by size:
877 653 27
PstI C_TGCA'G
Cuts at: 0 407 1073 1557
Size: 407 666 484
Fragments arranged by size:
666 484 407
SacI G_AGCT'C
Cuts at: 0 979 1391 1499 1557
Size: 979 412 108 58
Fragments arranged by size:
979 412 108 58
SfcI C'TryA_G
Cuts at: 0 403 1069 1557
Size: 403 666 488
Fragments arranged by size:
666 488 403
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1345 1557
Size: 1345 212
SmaI CCC'GGG
Cuts at: 0 657 1557
Size: 657 900
SmlI C'TyrA_G
Cuts at: 0 1390 1557
Size: 1390 167
StyI C'CwwG_G
Cuts at: 0 64 89 1557
Size: 64 25 1468
Fragments arranged by size:
1468 64 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 1557
Size: 130 1427
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 126 158 1354 1386 1557
Size: 37 32 57 32 1196 32 171
Fragments arranged by size:
1196 171 57 37 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 99 1557
Size: 99 1458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 687 1251 1557
Size: 687 564 306
Fragments arranged by size:
687 564 306
XmaI C'CCGG_G
Cuts at: 0 655 1557
Size: 655 902
XmnI GAAnn'nnTTC
Cuts at: 0 664 1557
Size: 664 893
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AlwNI ApaI AvaI
BamHI BanI BanII BbsI BbvCI BclI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI BssHII
BstXI BstYI Bsu36I BtgI BtgZI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I MmeI MscI MslI MspA1I NcoI PflMI
PfoI PmlI PpiI PshAI PspOMI PstI SacI SfcI
SfiI SmaI SmlI StyI TaqII TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BfrBI BglII BmtI BplI BsiWI
BspEI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I BtsI ClaI DraI DrdI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpuMI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XhoI ZraI