Restriction map for
pFN21AE6284
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspMI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRII EcoRV FseI FspI HaeIII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MseI MunI MvaI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
StuI SwaI Tru9I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 40 GTAGAC GT^[AC][GT]AC
AflIII 292 ACATGT A^C[GA][CT]GT
Aor51HI 440 AGCGCT AGC^GCT
AspHI 543 GTGCTC G[AT]GC[AT]^C
BamHI 462 GGATCC G^GATCC
BanII 133 GAGCCC G[GA]GC[CT]^C
BbsI 486 GAAGAC GAAGAC(2/6)
BbvI 3 GCTGC GCAGC(8/12)
BclI 71 TGATCA T^GATCA
BcnI 102 CCGGG CC^[GC]GG
BpmI 516 CTGGAG CTGGAG(16/14)
BpuAI 486 GAAGAC GAAGAC(2/6)
BsaBI 523 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 17 ACCGGA [AT]^CCGG[AT]
BsiHKAI 543 GTGCTC G[AT]GC[AT]^C
BsmAI 626 GAGAC GTCTC(1/5)
BsmI 756 GCATTC GAATGC(1/-1)
BspLU11I 292 ACATGT A^CATGT
BsrBI 31 CCGCTC CCGCTC(-3/-3)
BssKI 102 CCGGG ^CC[ACGT]GG
CfoI 441 GCGC GCG^C
DraII 143 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 613 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 153 CCATGG C^C[GA][CT]GG
Eco47III 440 AGCGCT AGC^GCT
EcoO109I 143 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 153 CCATGG C^C[AT][AT]GG
EcoT22I 721 ATGCAT ATGCA^T
FauI 180 GCGGG CCCGC(4/6)
FbaI 71 TGATCA T^GATCA
HaeII 440 AGCGCT [GA]GCGC^[CT]
HhaI 441 GCGC GCG^C
HinP1I 441 GCGC G^CGC
MaeII 389 ACGT A^CGT
MamI 523 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 486 GAAGA GAAGA(8/7)
MspA1I 61 CAGCTG C[AC]G^C[GT]G
MwoI 77 GCAAACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 102 CCGGG CC^[GC]GG
NcoI 153 CCATGG C^CATGG
NsiI 721 ATGCAT ATGCA^T
PleI 783 GAGTC GAGTC(4/5)
PpuMI 143 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 61 CAGCTG CAG^CTG
ScrFI 102 CCGGG CC^[ACGT]GG
SphI 26 GCATGC GCATG^C
SspI 234 AATATT AAT^ATT
StyI 153 CCATGG C^C[AT][AT]GG
TaqI 371 TCGA T^CGA
TfiI 454 GAATC G^A[AT]TC
TseI 3 GCTGC G^C[AT]GC
TthHB8I 371 TCGA T^CGA
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# TWO CUT ENZYMES ( 34 enzymes) #
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AciI 31 CCGC CCGC(-3/-1)
180 GCGG
AcsI 310 AAATTC [GA]^AATT[CT]
431 AAATTC
ApoI 310 AAATTC [GA]^AATT[CT]
431 AAATTC
AvaII 144 GGACC G^G[AT]CC
621 GGTCC
BfaI 206 CTAG C^TAG
215 CTAG
BmyI 133 GAGCCC G[AGT]GC[ACT]^C
543 GTGCTC
BsaJI 102 CCGGGG C^C[ACGT][ACGT]GG
153 CCATGG
BsiYI 148 CCTCACCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
173 CCGAGAAGCGG
BslI 148 CCTCACCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
173 CCGAGAAGCGG
BsmFI 498 GGGAC GGGAC(10/14)
226 GTCCC
Bsp1286I 133 GAGCCC G[AGT]GC[ACT]^C
543 GTGCTC
Bsp1407I 65 TGTACA T^GTACA
609 TGTACA
BsrDI 158 GCAATG GCAATG(2/0)
356 GCAATG
BsrGI 65 TGTACA T^GTACA
609 TGTACA
BstF5I 693 GGATG GGATG(2/0)
771 GGATG
BstXI 153 CCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
383 CCAAAGACGTGG
BstYI 20 GGATCT [GA]^GATC[CT]
462 GGATCC
Cac8I 26 GCATGC GC[ACGT]^[ACGT]GC
421 GCCTGC
Cfr13I 144 GGACC G^G[ACGT]CC
621 GGTCC
DdeI 458 CTGAG C^T[ACGT]AG
467 CTAAG
Fnu4HI 3 GCTGC GC^[ACGT]GC
30 GCCGC
FokI 693 GGATG GGATG(9/13)
771 GGATG
HapII 18 CCGG C^CGG
102 CCGG
HinfI 454 GAATC G^A[ACGT]TC
783 GAGTC
HpaII 18 CCGG C^CGG
102 CCGG
ItaI 3 GCTGC GC^[ACGT]GC
30 GCCGC
MaeI 206 CTAG C^TAG
215 CTAG
MflI 20 GGATCT [GA]^GATC[CT]
462 GGATCC
MslI 82 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
154 CATGGCAATG
MspI 18 CCGG C^CGG
102 CCGG
NlaIV 144 GGACCC GG[ACGT]^[ACGT]CC
462 GGATCC
Sau96I 144 GGACC G^G[ACGT]CC
621 GGTCC
SspBI 65 TGTACA T^GTACA
609 TGTACA
XhoII 20 GGATCT [GA]^GATC[CT]
462 GGATCC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AfaI AluI AlwI DpnII HphI
MaeIII MboI MnlI NdeII NlaIII
NspI RsaI Sau3AI Tsp45I Tsp509I