(Circular) (Six-base) MAPSORT of: pFN21AE6284.seq Check: 9725 from: 1 to: 5509 pFN21AE6284 5509 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2046 2852 1937 Size: 109 806 4594 Fragments arranged by size: 4594 806 109 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 3572 4956 5224 1632 Size: 944 996 1384 268 1917 Fragments arranged by size: 1917 1384 996 944 268 AfeI AGC'GCT Cuts at: 2447 2447 Size: 5509 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 2297 4408 5385 1203 Size: 1094 2111 977 1327 Fragments arranged by size: 2111 1327 1094 977 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 3252 4824 3252 Size: 1572 3937 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2315 2436 2818 3044 3196 5305 1493 Size: 822 121 382 226 152 2109 1697 Fragments arranged by size: 2109 1697 822 382 226 152 121 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2834 1870 Size: 73 891 4545 Fragments arranged by size: 4545 891 73 BamHI G'GATC_C Cuts at: 2467 2839 3206 2467 Size: 372 367 4770 Fragments arranged by size: 4770 372 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2830 4285 618 Size: 325 217 627 1043 1455 1842 Fragments arranged by size: 1842 1455 1043 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2828 1391 Size: 751 686 4072 Fragments arranged by size: 4072 751 686 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 928 Size: 971 599 3939 Fragments arranged by size: 3939 971 599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3700 3734 1903 Size: 34 50 34 1679 34 3678 Fragments arranged by size: 3678 1679 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4611 3401 Size: 1210 4299 BclI T'GATC_A Cuts at: 1548 2076 1548 Size: 528 4981 BfrBI ATG'CAT Cuts at: 2728 2728 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4120 136 Size: 107 122 71 631 605 2448 1525 Fragments arranged by size: 2448 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5504 5504 Size: 5509 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 3570 4726 1790 Size: 1780 1156 2573 Fragments arranged by size: 2573 1780 1156 BmgBI CAC'GTC Cuts at: 1318 2395 1318 Size: 1077 4432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3240 4198 442 Size: 416 53 83 399 24 1823 958 1753 Fragments arranged by size: 1823 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2542 4169 1239 Size: 1303 1627 2579 Fragments arranged by size: 2579 1627 1303 Bpu10I CC'TnA_GC Cuts at: 2472 2472 Size: 5509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3634 4499 4797 3634 Size: 865 298 4346 Fragments arranged by size: 4346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 882 Size: 617 2673 2219 Fragments arranged by size: 2673 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 2532 5503 2532 Size: 2971 2538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3316 3698 4333 275 Size: 53 83 186 936 1783 382 635 1451 Fragments arranged by size: 1783 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2022 3942 4340 4614 4761 5172 2022 Size: 1920 398 274 147 411 2359 Fragments arranged by size: 2359 1920 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2103 2133 2515 2545 1178 Size: 30 165 30 700 30 382 30 4142 Fragments arranged by size: 4142 700 382 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5509 BseYI C'CCAG_C Cuts at: 4712 5208 4712 Size: 496 5013 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4936 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 3720 4748 1540 Size: 275 186 958 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2552 3570 3655 4726 2552 Size: 1018 85 1071 3335 Fragments arranged by size: 3335 1071 1018 85 BsmI GAATG_Cn' Cuts at: 2761 3020 3113 2761 Size: 259 93 5157 Fragments arranged by size: 5157 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2552 2828 3570 3655 4726 1391 Size: 399 352 410 276 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 352 276 85 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 3398 5128 3398 Size: 1730 3779 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2038 3396 1394 Size: 429 215 1358 3507 Fragments arranged by size: 3507 1358 429 215 BsrDI GCAATG_nn' Cuts at: 65 1248 2170 2368 4004 4178 65 Size: 1183 922 198 1636 174 1396 Fragments arranged by size: 1636 1396 1183 922 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4153 4340 1700 Size: 110 2343 187 2869 Fragments arranged by size: 2869 2343 187 110 BsrGI T'GTAC_A Cuts at: 96 2070 2614 96 Size: 1974 544 2991 Fragments arranged by size: 2991 1974 544 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5509 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstXI CCAn_nnnn'nTGG Cuts at: 2165 2395 2165 Size: 230 5279 BstYI r'GATC_y Cuts at: 1972 2025 2467 2839 3206 3591 3608 5060 5146 Size: 53 442 372 367 385 17 1452 86 Cuts at: 5146 5158 5504 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 442 385 372 367 346 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2158 513 Size: 552 1093 3864 Fragments arranged by size: 3864 1093 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2445 487 Size: 683 56 1219 3551 Fragments arranged by size: 3551 1219 683 56 BtsI GCAGTG_nn' Cuts at: 3025 3819 3839 3025 Size: 794 20 4695 Fragments arranged by size: 4695 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DraIII CAC_nnn'GTG Cuts at: 2623 2623 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2956 3845 5474 Size: 54 1425 50 275 18 1126 889 1629 Cuts at: 5474 8 Size: 43 Fragments arranged by size: 1629 1425 1126 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 1537 Size: 275 1144 4090 Fragments arranged by size: 4090 1144 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3439 1353 Size: 2086 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4096 4470 4616 387 Size: 1064 138 2507 374 146 1280 Fragments arranged by size: 2507 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2542 2576 3572 4169 4956 5224 1239 Size: 393 910 34 996 597 787 268 1524 Fragments arranged by size: 1524 996 910 787 597 393 268 34 EcoO109I rG'GnC_Cy Cuts at: 2149 2149 Size: 5509 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FspI TGC'GCA Cuts at: 4015 4015 Size: 5509 HaeII r_GCGC'y Cuts at: 1678 2449 4656 1678 Size: 771 2207 2531 Fragments arranged by size: 2531 2207 771 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2214 2246 2515 2547 4150 Size: 32 135 32 642 32 269 32 1603 Cuts at: 4150 4182 4224 4256 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1603 642 269 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3099 669 Size: 988 281 915 246 3079 Fragments arranged by size: 3079 988 915 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5509 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4623 4807 1305 Size: 3318 184 2007 Fragments arranged by size: 3318 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3687 Fragments arranged by size: 3687 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2091 2163 3467 3826 3985 518 Size: 648 925 72 1304 359 159 2042 Fragments arranged by size: 2042 1304 925 648 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2068 2925 3602 4750 4995 1606 Size: 462 857 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 677 462 245 NcoI C'CATG_G Cuts at: 513 1065 2158 513 Size: 552 1093 3864 Fragments arranged by size: 3864 1093 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2956 2956 Size: 5509 NsiI A_TGCA'T Cuts at: 2730 2730 Size: 5509 NspI r_CATG'y Cuts at: 1132 1229 2035 2301 2728 2867 4412 1132 Size: 97 806 266 427 139 1545 2229 Fragments arranged by size: 2229 1545 806 427 266 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 2297 4408 2297 Size: 2111 3398 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2149 Size: 5509 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2598 2630 2598 Size: 32 5477 PstI C_TGCA'G Cuts at: 830 2861 830 Size: 2031 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 PvuII CAG'CTG Cuts at: 2068 2068 Size: 5509 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2857 3992 4673 4864 826 Size: 219 1812 1135 681 191 1471 Fragments arranged by size: 1812 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2836 1872 Size: 964 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3613 4514 4776 820 Size: 197 926 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2035 2867 1132 Size: 903 832 3774 Fragments arranged by size: 3774 903 832 SspI AAT'ATT Cuts at: 5 52 2241 3433 5 Size: 47 2189 1192 2081 Fragments arranged by size: 2189 2081 1192 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5509 StyI C'CwwG_G Cuts at: 513 1065 2158 2948 513 Size: 552 1093 790 3074 Fragments arranged by size: 3074 1093 790 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3553 3706 3891 4336 325 Size: 1255 1956 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 2070 2614 Size: 274 80 33 51 1443 75 18 544 Cuts at: 2614 2804 3755 5467 96 Size: 190 951 1712 138 Fragments arranged by size: 1712 1443 951 544 274 190 138 80 75 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 5469 5501 2103 Size: 32 3334 32 2111 Fragments arranged by size: 3334 2111 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2834 1870 Size: 964 4545 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI