Restriction map for
pFN21AE6284
(Linear) (Six-base) MAPSORT of: KIEE6284.seq Check: 3673 from: 1 to: 804
KIEE6284.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 41 804
Size: 41 763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 804
Size: 571 233
AfeI AGC'GCT
Cuts at: 0 442 804
Size: 442 362
AflIII A'CryG_T
Cuts at: 0 292 804
Size: 292 512
ApoI r'AATT_y
Cuts at: 0 310 431 804
Size: 310 121 373
Fragments arranged by size:
373 310 121
BamHI G'GATC_C
Cuts at: 0 462 804
Size: 462 342
BanII G_rGCy'C
Cuts at: 0 137 804
Size: 137 667
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 804
Size: 493 311
BclI T'GATC_A
Cuts at: 0 71 804
Size: 71 733
BfrBI ATG'CAT
Cuts at: 0 723 804
Size: 723 81
BmgBI CAC'GTC
Cuts at: 0 390 804
Size: 390 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 804
Size: 537 267
Bpu10I CC'TnA_GC
Cuts at: 0 467 804
Size: 467 337
BsaBI GATnn'nnATC
Cuts at: 0 527 804
Size: 527 277
BsaWI w'CCGG_w
Cuts at: 0 17 804
Size: 17 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 510 540 804
Size: 98 30 382 30 264
Fragments arranged by size:
382 264 98 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 547 804
Size: 547 257
BsmI GAATG_Cn'
Cuts at: 0 756 804
Size: 756 48
Bsp1286I G_dGCh'C
Cuts at: 0 137 547 804
Size: 137 410 257
Fragments arranged by size:
410 257 137
BsrBI CCG'CTC
Cuts at: 0 33 804
Size: 33 771
BsrDI GCAATG_nn'
Cuts at: 0 165 363 804
Size: 165 198 441
Fragments arranged by size:
441 198 165
BsrGI T'GTAC_A
Cuts at: 0 65 609 804
Size: 65 544 195
Fragments arranged by size:
544 195 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 160 390 804
Size: 160 230 414
Fragments arranged by size:
414 230 160
BstYI r'GATC_y
Cuts at: 0 20 462 804
Size: 20 442 342
Fragments arranged by size:
442 342 20
BtgI C'CryG_G
Cuts at: 0 153 804
Size: 153 651
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 440 804
Size: 440 364
DraIII CAC_nnn'GTG
Cuts at: 0 618 804
Size: 618 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 571 804
Size: 537 34 233
Fragments arranged by size:
537 233 34
EcoO109I rG'GnC_Cy
Cuts at: 0 144 804
Size: 144 660
HaeII r_GCGC'y
Cuts at: 0 444 804
Size: 444 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 510 542 804
Size: 209 32 269 32 262
Fragments arranged by size:
269 262 209 32 32
MslI CAynn'nnrTG
Cuts at: 0 86 158 804
Size: 86 72 646
Fragments arranged by size:
646 86 72
MspA1I CmG'CkG
Cuts at: 0 63 804
Size: 63 741
NcoI C'CATG_G
Cuts at: 0 153 804
Size: 153 651
NsiI A_TGCA'T
Cuts at: 0 725 804
Size: 725 79
NspI r_CATG'y
Cuts at: 0 30 296 723 804
Size: 30 266 427 81
Fragments arranged by size:
427 266 81 30
PciI A'CATG_T
Cuts at: 0 292 804
Size: 292 512
PpuMI rG'GwC_Cy
Cuts at: 0 144 804
Size: 144 660
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 593 625 804
Size: 593 32 179
Fragments arranged by size:
593 179 32
PvuII CAG'CTG
Cuts at: 0 63 804
Size: 63 741
SphI G_CATG'C
Cuts at: 0 30 804
Size: 30 774
SspI AAT'ATT
Cuts at: 0 236 804
Size: 236 568
StyI C'CwwG_G
Cuts at: 0 153 804
Size: 153 651
TatI w'GTAC_w
Cuts at: 0 47 65 609 799 804
Size: 47 18 544 190 5
Fragments arranged by size:
544 190 47 18 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 98 130 804
Size: 98 32 674
Fragments arranged by size:
674 98 32
Enzymes that do cut:
AccI AcuI AfeI AflIII ApoI BamHI BanII BbsI
BclI BfrBI BmgBI BpmI Bpu10I BsaBI BsaWI BsaXI
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI BstXI BstYI
BtgI BtgZI DraIII Eco57MI EcoO109I HaeII Hin4I MslI
MspA1I NcoI NsiI NspI PciI PpuMI PsrI PvuII
SphI SspI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI