################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeIII HapII HincII HindII HindIII HinfI HpaI HpaII ItaI KpnI Ksp632I KspI MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvaI MvnI NaeI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI ScrFI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcyI 44 GGCGCC G[GA]^CG[CT]C AfaI 123 GTAC GT^AC AluI 211 AGCT AG^CT AvaII 144 GGACC G^G[AT]CC BanI 44 GGCGCC G^G[CT][GA]CC BbeI 44 GGCGCC GGCGC^C BbrPI 148 CACGTG CAC^GTG BfaI 14 CTAG C^TAG BsaAI 148 CACGTG [CT]AC^GT[GA] BsaHI 44 GGCGCC G[GA]^CG[CT]C Bsp1407I 122 TGTACA T^GTACA BsrGI 122 TGTACA T^GTACA BsrI 169 ACTGG ACTGG(1/-1) Cac8I 47 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 144 GGACC G^G[ACGT]CC DdeI 52 CTTAG C^T[ACGT]AG HaeII 44 GGCGCC [GA]GCGC^[CT] HgaI 60 GACGC GACGC(5/10) Hin1I 44 GGCGCC G[GA]^CG[CT]C KasI 44 GGCGCC G^GCGCC MaeI 14 CTAG C^TAG MslI 114 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 104 GCTCGGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 44 GGCGCC GG^CGCC NlaIII 174 CATG CATG^ NlaIV 44 GGCGCC GG[ACGT]^[ACGT]CC PmaCI 148 CACGTG CAC^GTG PmlI 148 CACGTG CAC^GTG RsaI 123 GTAC GT^AC Sau96I 144 GGACC G^G[ACGT]CC SfoI 44 GGCGCC GGC^GCC SspBI 122 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AspHI 37 GAGCAC G[AT]GC[AT]^C 111 GAGCAC BmyI 37 GAGCAC G[AGT]GC[ACT]^C 111 GAGCAC BsiHKAI 37 GAGCAC G[AT]GC[AT]^C 111 GAGCAC Bsp1286I 37 GAGCAC G[AGT]GC[ACT]^C 111 GAGCAC CfoI 45 GCGC GCG^C 69 GCGC HhaI 45 GCGC GCG^C 69 GCGC HinP1I 45 GCGC G^CGC 69 GCGC HphI 108 GGTGA GGTGA(8/7) 83 TCACC MaeII 74 ACGT A^CGT 149 ACGT MnlI 6 CCTC CCTC(7/6) 86 CCTC SfaNI 66 GATGC GCATC(5/9) 204 GATGC TspRI 18 CAGTG CA[GC]TG[ACGT][ACGT]^ 114 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 0 enzymes) # #########################################