(Circular) (Six-base) MAPSORT of: pFN21AE5184.seq Check: 142 from: 1 to: 4921 pFN21AE5184 4921 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2731 278 Size: 53 83 186 936 1195 2468 Fragments arranged by size: 2468 1195 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2264 1937 Size: 327 4594 Acc65I G'GTAC_C Cuts at: 2242 2242 Size: 4921 AclI AA'CG_TT Cuts at: 1563 3048 3421 1563 Size: 1485 373 3063 Fragments arranged by size: 3063 1485 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2984 4368 4636 1632 Size: 1352 1384 268 1917 Fragments arranged by size: 1917 1384 1352 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4724 AflIII A'CryG_T Cuts at: 1203 3820 4797 1203 Size: 2617 977 1327 Fragments arranged by size: 2617 1327 977 AgeI A'CCGG_T Cuts at: 3752 3752 Size: 4921 AhdI GACnn_n'nnGTC Cuts at: 3650 3650 Size: 4921 AleI CACnn'nnGTG Cuts at: 1166 2123 1166 Size: 957 3964 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4921 AlwNI CAG_nnn'CTG Cuts at: 2664 4236 2664 Size: 1572 3349 ApaLI G'TGCA_C Cuts at: 2978 4134 2978 Size: 1156 3765 ApoI r'AATT_y Cuts at: 1493 2230 2456 2608 4717 1493 Size: 737 226 152 2109 1697 Fragments arranged by size: 2109 1697 737 226 152 AseI AT'TA_AT Cuts at: 160 1032 3475 160 Size: 872 2443 1606 Fragments arranged by size: 2443 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4921 AvaI C'yCGr_G Cuts at: 1870 1943 2246 1870 Size: 73 303 4545 Fragments arranged by size: 4545 303 73 BamHI G'GATC_C Cuts at: 2251 2618 2251 Size: 367 4554 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2242 3697 618 Size: 325 217 627 262 193 1455 1842 Fragments arranged by size: 1842 1455 627 325 262 217 193 BanII G_rGCy'C Cuts at: 1391 2240 1391 Size: 849 4072 BbeI G_GCGC'C Cuts at: 2053 2053 Size: 4921 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3112 3146 1903 Size: 34 1175 34 3678 Fragments arranged by size: 3678 1175 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2813 4023 2813 Size: 1210 3711 BclI T'GATC_A Cuts at: 1548 1548 Size: 4921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3532 136 Size: 107 122 71 631 605 1860 1525 Fragments arranged by size: 1860 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4916 4916 Size: 4921 BlpI GC'TnA_GC Cuts at: 2338 2338 Size: 4921 Bme1580I G_kGCm'C Cuts at: 1790 2982 4138 1790 Size: 1192 1156 2573 Fragments arranged by size: 2573 1192 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2652 3610 442 Size: 416 53 83 399 24 1235 958 1753 Fragments arranged by size: 1753 1235 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4921 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3581 1239 Size: 2342 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3046 3911 4209 3046 Size: 865 298 3758 Fragments arranged by size: 3758 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3584 882 Size: 617 2085 2219 Fragments arranged by size: 2219 2085 617 BsaAI yAC'GTr Cuts at: 493 1204 2155 2712 493 Size: 711 951 557 2702 Fragments arranged by size: 2702 951 711 557 BsaBI GATnn'nnATC Cuts at: 4915 4915 Size: 4921 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2050 2728 3110 3745 Size: 53 83 186 936 517 678 382 635 Cuts at: 3745 275 Size: 1451 Fragments arranged by size: 1451 936 678 635 517 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3354 3752 4026 4173 4584 3354 Size: 398 274 147 411 3691 Fragments arranged by size: 3691 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4696 Fragments arranged by size: 4696 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4921 BseYI C'CCAG_C Cuts at: 4124 4620 4124 Size: 496 4425 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2371 3132 4160 1540 Size: 275 186 370 761 1028 2301 Fragments arranged by size: 2301 1028 761 370 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2120 2982 3067 4138 2046 Size: 74 862 85 1071 2829 Fragments arranged by size: 2829 1071 862 85 74 BsmI GAATG_Cn' Cuts at: 2432 2525 2432 Size: 93 4828 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2120 2240 2982 3067 4138 1391 Size: 399 256 74 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 256 120 85 74 BspEI T'CCGG_A Cuts at: 4584 4584 Size: 4921 BspHI T'CATG_A Cuts at: 2810 4540 2810 Size: 1730 3191 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2276 844 Size: 1432 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2808 1394 Size: 429 985 3507 Fragments arranged by size: 3507 985 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3416 3590 65 Size: 1183 2168 174 1396 Fragments arranged by size: 2168 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3565 3752 1700 Size: 110 1755 187 2869 Fragments arranged by size: 2869 1755 187 110 BsrGI T'GTAC_A Cuts at: 96 2127 96 Size: 2031 2890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4921 BssSI C'ACGA_G Cuts at: 2981 3993 2981 Size: 1012 3909 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4921 BstBI TT'CG_AA Cuts at: 3725 3725 Size: 4921 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4921 BstYI r'GATC_y Cuts at: 1972 2251 2618 3003 3020 4472 4558 4570 4916 Size: 279 367 385 17 1452 86 12 346 Cuts at: 4916 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4369 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4182 Fragments arranged by size: 4182 683 56 BtsI GCAGTG_nn' Cuts at: 2029 2118 2437 3231 3251 2029 Size: 89 319 794 20 3699 Fragments arranged by size: 3699 794 319 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4886 DraI TTT'AAA Cuts at: 2225 2225 Size: 4921 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3928 809 Size: 507 2612 1802 Fragments arranged by size: 2612 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2368 3257 4886 Size: 54 1425 50 275 18 538 889 1629 Cuts at: 4886 8 Size: 43 Fragments arranged by size: 1629 1425 889 538 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2368 1537 Size: 275 556 4090 Fragments arranged by size: 4090 556 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2851 1353 Size: 1498 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3508 3882 4028 387 Size: 1064 138 1919 374 146 1280 Fragments arranged by size: 1919 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2984 3581 4368 4636 1239 Size: 393 1352 597 787 268 1524 Fragments arranged by size: 1524 1352 787 597 393 268 EcoRI G'AATT_C Cuts at: 2230 2608 2230 Size: 378 4543 FspI TGC'GCA Cuts at: 3427 3427 Size: 4921 HaeII r_GCGC'y Cuts at: 1678 2053 4068 1678 Size: 375 2015 2531 Fragments arranged by size: 2531 2015 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3562 3594 3636 3668 1373 Size: 32 135 32 1990 32 42 32 2626 Fragments arranged by size: 2626 1990 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2265 2511 669 Size: 988 281 327 246 3079 Fragments arranged by size: 3079 988 327 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4921 HpaI GTT'AAC Cuts at: 2511 2511 Size: 4921 KasI G'GCGC_C Cuts at: 2049 2049 Size: 4921 KpnI G_GTAC'C Cuts at: 2246 2246 Size: 4921 MfeI C'AATT_G Cuts at: 2520 2520 Size: 4921 MluI A'CGCG_T Cuts at: 4797 4797 Size: 4921 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4035 4219 1305 Size: 2730 184 2007 Fragments arranged by size: 2730 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3099 Fragments arranged by size: 3099 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2879 3238 3397 518 Size: 648 957 756 359 159 2042 Fragments arranged by size: 2042 957 756 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2337 3014 4162 4407 1606 Size: 731 677 1148 245 2120 Fragments arranged by size: 2120 1148 731 677 245 NarI GG'CG_CC Cuts at: 2050 2050 Size: 4921 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4369 NdeI CA'TA_TG Cuts at: 387 387 Size: 4921 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4921 NotI GC'GGCC_GC Cuts at: 2368 2368 Size: 4921 NspI r_CATG'y Cuts at: 1132 1229 2279 3824 1132 Size: 97 1050 1545 2229 Fragments arranged by size: 2229 1545 1050 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4921 PciI A'CATG_T Cuts at: 3820 3820 Size: 4921 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4921 PmeI GTTT'AAAC Cuts at: 2225 2225 Size: 4921 PmlI CAC'GTG Cuts at: 2155 2155 Size: 4921 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2990 3022 4503 4535 2990 Size: 32 1481 32 3376 Fragments arranged by size: 3376 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4921 PsiI TTA'TAA Cuts at: 2491 2491 Size: 4921 PstI C_TGCA'G Cuts at: 830 2273 830 Size: 1443 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4921 SalI G'TCGA_C Cuts at: 1936 2263 1936 Size: 327 4594 SbfI CC_TGCA'GG Cuts at: 2273 2273 Size: 4921 ScaI AGT'ACT Cuts at: 3169 4881 3169 Size: 1712 3209 SfcI C'TryA_G Cuts at: 826 1045 2269 3404 4085 4276 826 Size: 219 1224 1135 681 191 1471 Fragments arranged by size: 1471 1224 1135 681 219 191 SfoI GGC'GCC Cuts at: 2051 2051 Size: 4921 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4921 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4921 SmaI CCC'GGG Cuts at: 1872 2248 1872 Size: 376 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3025 3926 4188 820 Size: 197 926 1082 901 262 1553 Fragments arranged by size: 1553 1082 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2712 493 Size: 2219 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4921 SphI G_CATG'C Cuts at: 1132 2279 1132 Size: 1147 3774 SspI AAT'ATT Cuts at: 5 52 2845 5 Size: 47 2793 2081 Fragments arranged by size: 2793 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4921 StyI C'CwwG_G Cuts at: 513 1065 2360 513 Size: 552 1295 3074 Fragments arranged by size: 3074 1295 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2948 2965 3118 3303 3748 325 Size: 1255 1368 17 153 185 445 1498 Fragments arranged by size: 1498 1368 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 3167 4879 Size: 274 80 33 51 1443 150 1040 1712 Cuts at: 4879 96 Size: 138 Fragments arranged by size: 1712 1443 1040 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4881 4913 4881 Size: 32 4889 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4921 XbaI T'CTAG_A Cuts at: 2257 2257 Size: 4921 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4921 XmaI C'CCGG_G Cuts at: 1870 2246 1870 Size: 376 4545 XmnI GAAnn'nnTTC Cuts at: 3050 3050 Size: 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2729 276 Size: 53 83 186 936 1195 2468 Fragments arranged by size: 2468 1195 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI