(Linear) (Six-base) MAPSORT of: KIEE5184.seq  Check: 9340  from: 1  to: 216



KIEE5184.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AleI CACnn'nnGTG

Cuts at:      0     118     216 
   Size:        118      98
 
BanI G'GyrC_C

Cuts at:      0      44     216 
   Size:         44     172
 
BbeI G_GCGC'C

Cuts at:      0      48     216 
   Size:         48     168
 
BsaAI yAC'GTr

Cuts at:      0     150     216 
   Size:        150      66
 
BsaHI Gr'CG_yC

Cuts at:      0      45     216 
   Size:         45     171
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     115     216 
   Size:         41      74     101

  Fragments arranged by size: 

                 101      74      41
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     115     216 
   Size:         41      74     101

  Fragments arranged by size: 

                 101      74      41
 
BsrGI T'GTAC_A

Cuts at:      0     122     216 
   Size:        122      94
 
BtsI GCAGTG_nn'

Cuts at:      0      24     113     216 
   Size:         24      89     103

  Fragments arranged by size: 

                 103      89      24
 
HaeII r_GCGC'y

Cuts at:      0      48     216 
   Size:         48     168
 
KasI G'GCGC_C

Cuts at:      0      44     216 
   Size:         44     172
 
MslI CAynn'nnrTG

Cuts at:      0     118     216 
   Size:        118      98
 
NarI GG'CG_CC

Cuts at:      0      45     216 
   Size:         45     171
 
PmlI CAC'GTG

Cuts at:      0     150     216 
   Size:        150      66
 
SfoI GGC'GCC

Cuts at:      0      46     216 
   Size:         46     170
 
TatI w'GTAC_w

Cuts at:      0     122     216 
   Size:        122      94
 

 Enzymes that do cut:

     AleI     BanI     BbeI    BsaAI    BsaHI  BsiHKAI Bsp1286I    BsrGI
     BtsI    HaeII     KasI     MslI     NarI     PmlI     SfoI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI