################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HphI KasI KpnI KspI MamI MluI MroI MslI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Tsp45I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 44 GACGTC GACGT^C AfaI 1385 GTAC GT^AC AflIII 1136 ACACGT A^C[GA][CT]GT AseI 976 ATTAAT AT^TAAT AsnI 976 ATTAAT AT^TAAT AspHI 406 GAGCTC G[AT]GC[AT]^C AspI 1279 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 752 TGGCCA TGG^CCA BamHI 426 GGATCC G^GATCC BbvCI 1093 GCTGAGG CCTCAGC(-5/-2) BcnI 1036 CCGGG CC^[GC]GG BglII 1369 AGATCT A^GATCT BlpI 938 GCTCAGC GC^T[ACGT]AGC Bpu1102I 938 GCTCAGC GC^T[ACGT]AGC BsaI 472 GGTCTC GGTCTC(1/5) BseRI 920 GAGGAG GAGGAG(10/8) BsiHKAI 406 GAGCTC G[AT]GC[AT]^C BsmFI 1277 GGGAC GGGAC(10/14) BsmI 1396 GCATTC GAATGC(1/-1) Bsp1407I 1384 TGTACA T^GTACA BspHI 902 TCATGA T^CATGA BsrBI 461 GAGCGG CCGCTC(-3/-3) BsrDI 730 GCAATG GCAATG(2/0) BsrGI 1384 TGTACA T^GTACA BstBI 1303 TTCGAA TT^CGAA CelII 938 GCTCAGC GC^T[ACGT]AGC CfoI 1120 GCGC GCG^C DraII 101 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 1184 CCATGG C^C[GA][CT]GG EcoO109I 101 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 1408 GAATTC G^AATTC EcoT14I 1184 CCATGG C^C[AT][AT]GG HhaI 1120 GCGC GCG^C HinP1I 1120 GCGC G^CGC HincII 1348 GTTAAC GT[CT]^[GA]AC HindII 1348 GTTAAC GT[CT]^[GA]AC HindIII 961 AAGCTT A^AGCTT HpaI 1348 GTTAAC GTT^AAC MfeI 601 CAATTG C^AATTG MluNI 752 TGGCCA TGG^CCA MscI 752 TGGCCA TGG^CCA MunI 601 CAATTG C^AATTG NciI 1036 CCGGG CC^[GC]GG NcoI 1184 CCATGG C^CATGG NspI 1381 GCATGT [GA]CATG^[CT] NspV 1303 TTCGAA TT^CGAA PflFI 1279 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 704 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 976 ATTAAT AT^TAAT RcaI 902 TCATGA T^CATGA RsaI 1385 GTAC GT^AC SacI 406 GAGCTC GAGCT^C SfuI 1303 TTCGAA TT^CGAA SspBI 1384 TGTACA T^GTACA SspI 979 AATATT AAT^ATT StuI 551 AGGCCT AGG^CCT StyI 1184 CCATGG C^C[AT][AT]GG Tth111I 1279 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Asp700I 116 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1404 GAAAGAATTC BanI 1180 GGTGCC G^G[CT][GA]CC 1207 GGTGCC BanII 406 GAGCTC G[GA]GC[CT]^C 769 GAGCCC BfaI 292 CTAG C^TAG 677 CTAG BpmI 403 CTGGAG CTGGAG(16/14) 1287 CTGGAG BsgI 161 GTGCAG GTGCAG(16/14) 384 GTGCAG EaeI 50 TGGCCG [CT]^GGCC[GA] 752 TGGCCA FauI 25 GCGGG CCCGC(4/6) 379 GCGGG MaeI 292 CTAG C^TAG 677 CTAG MaeIII 1127 GTTAC ^GT[ACGT]AC 1414 GTAAC PflMI 600 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 707 CCAAGTCTTGG PstI 1373 CTGCAG CTGCA^G 1391 CTGCAG PvuII 314 CAGCTG CAG^CTG 1388 CAGCTG SfcI 1373 CTGCAG C^T[GA][CT]AG 1391 CTGCAG Van91I 600 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 707 CCAAGTCTTGG XmnI 116 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1404 GAAAGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AcyI AluI AlwI ApoI AvaII BbsI BbvI BmyI BpuAI BsaHI BsaJI BsiYI BslI BsmAI BsmBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII ItaI Ksp632I MaeII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII