Restriction map for
pFN21AE5663
(Circular) (Six-base) MAPSORT of: pFN21AE5663.seq Check: 6410 from: 1 to: 6262
pFN21AE5663 6262 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2053 4072 278
Size: 53 83 186 936 517 2019 2468
Fragments arranged by size:
2468 2019 936 517 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3605 1937
Size: 1668 4594
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 4325 5709 5977 1632
Size: 626 2067 1384 268 1917
Fragments arranged by size:
2067 1917 1384 626 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 3141 5161 6138 1203
Size: 1938 2020 977 1327
Fragments arranged by size:
2020 1938 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2404 2956 1184
Size: 1220 552 4490
Fragments arranged by size:
4490 1220 552
AlwNI CAG_nnn'CTG
Cuts at: 4005 5577 4005
Size: 1572 4690
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2176 2793 3413 3571 3797 3949 6058 1493
Size: 683 617 620 158 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 620 617 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 2982 4816 160
Size: 872 1950 1834 1606
Fragments arranged by size:
1950 1834 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 3587 1870
Size: 73 1644 4545
Fragments arranged by size:
4545 1644 73
BamHI G'GATC_C
Cuts at: 2431 3592 3959 2431
Size: 1161 367 4734
Fragments arranged by size:
4734 1161 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3185 3212 3583 5038 618
Size: 325 217 627 1398 27 371 1455 1842
Fragments arranged by size:
1842 1455 1398 627 371 325 217 27
BanII G_rGCy'C
Cuts at: 1391 2415 2778 3581 1391
Size: 1024 363 803 4072
Fragments arranged by size:
4072 1024 803 363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 2776 3077 928
Size: 971 356 521 301 4113
Fragments arranged by size:
4113 971 521 356 301
BbvCI CC'TCA_GC
Cuts at: 3099 3099
Size: 6262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4873 136
Size: 107 122 71 631 605 3201 1525
Fragments arranged by size:
3201 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3374 6257 3374
Size: 2883 3379
BlpI GC'TnA_GC
Cuts at: 2944 3679 2944
Size: 735 5527
Bme1580I G_kGCm'C
Cuts at: 1790 3439 4323 5479 1790
Size: 1649 884 1156 2573
Fragments arranged by size:
2573 1649 1156 884
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2774 3228 3513
Size: 416 53 83 399 24 1357 454 285
Cuts at: 3513 3993 4951 442
Size: 480 958 1753
Fragments arranged by size:
1753 1357 958 480 454 416 399 285
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3318 3318
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 3313 4922 1239
Size: 1190 884 1609 2579
Fragments arranged by size:
2579 1609 1190 884
Bpu10I CC'TnA_GC
Cuts at: 2448 3099 2448
Size: 651 5611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4387 5252 5550 4387
Size: 865 298 5099
Fragments arranged by size:
5099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 4925 882
Size: 617 984 2442 2219
Fragments arranged by size:
2442 2219 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 2618 2636 4069
Size: 53 83 186 936 517 568 18 1433
Cuts at: 4069 4451 5086 275
Size: 382 635 1451
Fragments arranged by size:
1451 1433 936 635 568 517 382 186
83 53 18
BsaWI w'CCGG_w
Cuts at: 4695 5093 5367 5514 5925 4695
Size: 398 274 147 411 5032
Fragments arranged by size:
5032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2402 2432 1178
Size: 30 165 30 662 30 307 30 5008
Fragments arranged by size:
5008 662 307 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2940 1186
Size: 1754 4508
BseYI C'CCAG_C
Cuts at: 2285 3020 5465 5961 2285
Size: 735 2445 496 2586
Fragments arranged by size:
2586 2445 735 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2187 2410 1163
Size: 573 451 223 5015
Fragments arranged by size:
5015 573 451 223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 4323 4408 5479 2415
Size: 1908 85 1071 3198
Fragments arranged by size:
3198 1908 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3773 3866 3401
Size: 372 93 5797
Fragments arranged by size:
5797 372 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2056 2436 2609 2056
Size: 380 173 5709
Fragments arranged by size:
5709 380 173
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2778 3439 3581 4323 4408 5479
Size: 399 625 363 661 142 742 85 1071
Cuts at: 5479 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 661 625 399 363 142
85
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 2907 4151 5881 2907
Size: 1244 1730 3288
Fragments arranged by size:
3288 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2468 4149 1394
Size: 429 645 1681 3507
Fragments arranged by size:
3507 1681 645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 4757 4931 65
Size: 1183 1494 2015 174 1396
Fragments arranged by size:
2015 1494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4906 5093 1700
Size: 110 3096 187 2869
Fragments arranged by size:
3096 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3389 96
Size: 3293 2969
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 3309 5066 3309
Size: 1757 4505
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstYI r'GATC_y
Cuts at: 1972 2431 2765 3374 3592 3959 4344 4361 5813
Size: 459 334 609 218 367 385 17 1452
Cuts at: 5813 5899 5911 6257 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 609 459 385 367 346 334
218 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3189 513
Size: 552 2124 3586
Fragments arranged by size:
3586 2124 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 3778 4572 4592 3778
Size: 794 20 5448
Fragments arranged by size:
5448 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2757 3709
Size: 54 1425 50 275 18 225 702 952
Cuts at: 3709 4598 6227 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 952 889 702 275 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 3263 3311 4192 1353
Size: 672 1238 48 881 3423
Fragments arranged by size:
3423 1238 881 672 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2413
Size: 6262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2429 3313 4325 4922 5709 5977
Size: 393 626 171 884 1012 597 787 268
Cuts at: 5977 1239
Size: 1524
Fragments arranged by size:
1524 1012 884 787 626 597 393 268
171
EcoO109I rG'GnC_Cy
Cuts at: 2107 2107
Size: 6262
EcoRI G'AATT_C
Cuts at: 3413 3571 3949 3413
Size: 158 378 5726
Fragments arranged by size:
5726 378 158
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 5409 1678
Size: 3731 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2065 2097 2402
Size: 32 135 32 456 32 5 32 305
Cuts at: 2402 2434 2733 2765 4903 4935 4977 5009 1373
Size: 32 299 32 2138 32 42 32 2626
Fragments arranged by size:
2626 2138 456 305 299 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3355 3606 3852 669
Size: 988 281 1417 251 246 3079
Fragments arranged by size:
3079 1417 988 281 251 246
HindIII A'AGCT_T
Cuts at: 748 2966 748
Size: 2218 4044
HpaI GTT'AAC
Cuts at: 3355 3852 3355
Size: 497 5765
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 2606 3861 2606
Size: 1255 5007
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2071 5376 5560 1305
Size: 766 3305 184 2007
Fragments arranged by size:
3305 2007 766 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 10
Size: 54 1425 343 927 3513
Fragments arranged by size:
3513 1425 927 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4220 4579 4738 518
Size: 648 3054 359 159 2042
Fragments arranged by size:
3054 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2321 2384 3024 3395 3678 4355 5503 5748
Size: 715 63 640 371 283 677 1148 245
Cuts at: 5748 1606
Size: 2120
Fragments arranged by size:
2120 1148 715 677 640 371 283 245
63
NcoI C'CATG_G
Cuts at: 513 1065 3189 513
Size: 552 2124 3586
Fragments arranged by size:
3586 2124 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 3390 3620 5165 1132
Size: 97 2161 230 1545 2229
Fragments arranged by size:
2229 2161 1545 230 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2611 2718 1108
Size: 1503 107 4652
Fragments arranged by size:
4652 1503 107
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2713 1613
Size: 1100 5162
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PstI C_TGCA'G
Cuts at: 830 3382 3400 3614 830
Size: 2552 18 214 3478
Fragments arranged by size:
3478 2552 214 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 2321 3395 2321
Size: 1074 5188
SacI G_AGCT'C
Cuts at: 2415 2415
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SfcI C'TryA_G
Cuts at: 826 1045 3378 3396 3610 4745 5426 5617 826
Size: 219 2333 18 214 1135 681 191 1471
Fragments arranged by size:
2333 1471 1135 681 219 214 191 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3589 1872
Size: 1717 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4366 5267 5529 820
Size: 197 926 2423 901 262 1553
Fragments arranged by size:
2423 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 2986 4186 5
Size: 47 2934 1200 2081
Fragments arranged by size:
2934 2081 1200 47
StuI AGG'CCT
Cuts at: 1517 2558 1517
Size: 1041 5221
StyI C'CwwG_G
Cuts at: 513 1065 3189 3701 513
Size: 552 2124 512 3074
Fragments arranged by size:
3074 2124 552 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4289 4306 4459 4644 5089 325
Size: 1255 2709 17 153 185 445 1498
Fragments arranged by size:
2709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3389 4508 6220
Size: 274 80 33 51 1443 1412 1119 1712
Cuts at: 6220 96
Size: 138
Fragments arranged by size:
1712 1443 1412 1119 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6222 6254 6222
Size: 32 6230
Tth111I GACn'n_nGTC
Cuts at: 1316 3287 1316
Size: 1971 4291
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3587 1870
Size: 1717 4545
XmnI GAAnn'nnTTC
Cuts at: 2125 3413 4391 2125
Size: 1288 978 3996
Fragments arranged by size:
3996 1288 978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2051 4070 276
Size: 53 83 186 936 517 2019 2468
Fragments arranged by size:
2468 2019 936 517 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI