################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 979 GACGTC GACGT^C AccI 339 GTCGAC GT^[AC][GT]AC AccIII 239 TCCGGA T^CCGGA AcsI 1045 AAATTC [GA]^AATT[CT] AhdI 349 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 476 AGCGCT AGC^GCT ApaI 2014 GGGCCC GGGCC^C ApoI 1045 AAATTC [GA]^AATT[CT] AspEI 349 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1408 GGATCC G^GATCC BbvCI 1930 GCTGAGG CCTCAGC(-5/-2) BciVI 618 GGATAC GTATCC(6/5) BglI 2007 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1620 GGTCTC GGTCTC(1/5) BsaWI 239 TCCGGA [AT]^CCGG[AT] BseAI 239 TCCGGA T^CCGGA BspEI 239 TCCGGA T^CCGGA BsrDI 2120 GCAATG GCAATG(2/0) BstXI 800 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1946 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 349 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 476 AGCGCT AGC^GCT KspI 2303 CCGCGG CCGC^GG MamI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 239 TCCGGA T^CCGGA MseI 2723 TTAA T^TAA MslI 483 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 390 CCATGG C^CATGG NruI 2546 TCGCGA TCG^CGA PflMI 120 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1659 GAGCTC GAGCT^C SacII 2303 CCGCGG CCGC^GG SalI 339 GTCGAC G^TCGAC SapI 945 GCTCTTC GCTCTTC(1/4) SexAI 431 ACCTGGT A^CC[AT]GGT SmaI 1580 CCCGGG CCC^GGG Tru9I 2723 TTAA T^TAA Tsp509I 1046 AATT ^AATT Van91I 120 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 834 TCTAGA T^CTAGA XmaCI 1580 CCCGGG C^CCGGG XmaI 1580 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 609 GTAC GT^AC 2682 GTAC AlwNI 163 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1679 CAGCTACTG AocI 984 CCTGAGG CC^T[ACGT]AGG 1726 CCTGAGG Asp700I 3 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2328 GAAGTCATTC BalI 491 TGGCCA TGG^CCA 1293 TGGCCA BbeI 1970 GGCGCC GGCGC^C 2062 GGCGCC BbsI 696 GAAGAC GAAGAC(2/6) 1036 GAAGAC BcgI 2011 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1904 GCAGCATGATCG BpuAI 696 GAAGAC GAAGAC(2/6) 1036 GAAGAC BsmBI 1201 GAGACG CGTCTC(1/5) 1915 GAGACG BsrBI 1358 CCGCTC CCGCTC(-3/-3) 2175 GAGCGG BssSI 232 CACGAG CACGAG(-5/-1) 268 CTCGTG BstAPI 23 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 510 GCACACCCTGC Bsu36I 984 CCTGAGG CC^T[ACGT]AGG 1726 CCTGAGG Eco81I 984 CCTGAGG CC^T[ACGT]AGG 1726 CCTGAGG HgaI 1977 GACGC GACGC(5/10) 2618 GCGTC HincII 339 GTCGAC GT[CT]^[GA]AC 2569 GTCAAC HindII 339 GTCGAC GT[CT]^[GA]AC 2569 GTCAAC KasI 1970 GGCGCC G^GCGCC 2062 GGCGCC MaeII 785 ACGT A^CGT 980 ACGT MluNI 491 TGGCCA TGG^CCA 1293 TGGCCA MscI 491 TGGCCA TGG^CCA 1293 TGGCCA NarI 1970 GGCGCC GG^CGCC 2062 GGCGCC RsaI 609 GTAC GT^AC 2682 GTAC SfoI 1970 GGCGCC GGC^GCC 2062 GGCGCC StuI 196 AGGCCT AGG^CCT 669 AGGCCT XmnI 3 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2328 GAAGTCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII