(Circular) (Six-base) MAPSORT of: pFN21AE5827.seq Check: 7875 from: 1 to: 7456 pFN21AE5827 7456 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3709 3709 Size: 7456 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2988 5266 278 Size: 53 83 186 936 1452 2278 2468 Fragments arranged by size: 2468 2278 1452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2345 4799 1937 Size: 408 2454 4594 Fragments arranged by size: 4594 2454 408 Acc65I G'GTAC_C Cuts at: 4777 4777 Size: 7456 AclI AA'CG_TT Cuts at: 1563 5583 5956 1563 Size: 4020 373 3063 Fragments arranged by size: 4020 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3021 3429 4258 5519 6903 7171 1632 Size: 1389 408 829 1261 1384 268 1917 Fragments arranged by size: 1917 1389 1384 1261 829 408 268 AfeI AGC'GCT Cuts at: 2483 2483 Size: 7456 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7259 AflIII A'CryG_T Cuts at: 1203 6355 7332 1203 Size: 5152 977 1327 Fragments arranged by size: 5152 1327 977 AgeI A'CCGG_T Cuts at: 6287 6287 Size: 7456 AhdI GACnn_n'nnGTC Cuts at: 2359 6185 2359 Size: 3826 3630 AleI CACnn'nnGTG Cuts at: 1166 2492 1166 Size: 1326 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3467 4019 1184 Size: 2283 552 4621 Fragments arranged by size: 4621 2283 552 AlwNI CAG_nnn'CTG Cuts at: 2173 3689 5199 6771 2173 Size: 1516 1510 1572 2858 Fragments arranged by size: 2858 1572 1516 1510 ApaI G_GGCC'C Cuts at: 4023 4023 Size: 7456 ApaLI G'TGCA_C Cuts at: 5513 6669 5513 Size: 1156 6300 ApoI r'AATT_y Cuts at: 1493 3050 4765 4991 5143 7252 1493 Size: 1557 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 1557 226 152 AseI AT'TA_AT Cuts at: 160 1032 6010 160 Size: 872 4978 1606 Fragments arranged by size: 4978 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 AvaI C'yCGr_G Cuts at: 1870 1943 2068 3585 3787 4014 4781 1870 Size: 73 125 1517 202 227 767 4545 Fragments arranged by size: 4545 1517 767 227 202 125 73 BamHI G'GATC_C Cuts at: 3413 4786 5153 3413 Size: 1373 367 5716 Fragments arranged by size: 5716 1373 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2783 3138 3260 3975 4067 Size: 325 217 627 996 355 122 715 92 Cuts at: 4067 4777 6232 618 Size: 710 1455 1842 Fragments arranged by size: 1842 1455 996 715 710 627 355 325 217 122 92 BanII G_rGCy'C Cuts at: 1391 2025 3291 3668 4014 4023 4303 4671 4775 Size: 634 1266 377 346 9 280 368 104 Cuts at: 4775 1391 Size: 4072 Fragments arranged by size: 4072 1266 634 377 368 346 280 104 9 BbeI G_GCGC'C Cuts at: 3979 4071 3979 Size: 92 7364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2708 3048 928 Size: 971 809 340 5336 Fragments arranged by size: 5336 971 809 340 BbvCI CC'TCA_GC Cuts at: 3936 3936 Size: 7456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 4005 4039 5647 5681 1903 Size: 34 1961 34 73 34 1608 34 3678 Fragments arranged by size: 3678 1961 1608 73 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2617 5348 6558 2617 Size: 2731 1210 3515 Fragments arranged by size: 3515 2731 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4018 6067 136 Size: 107 122 71 631 605 2346 2049 1525 Fragments arranged by size: 2346 2049 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2842 3607 4533 7451 2842 Size: 765 926 2918 2847 Fragments arranged by size: 2918 2847 926 765 BlpI GC'TnA_GC Cuts at: 4873 4873 Size: 7456 Bme1580I G_kGCm'C Cuts at: 1790 4023 5517 6673 1790 Size: 2233 1494 1156 2573 Fragments arranged by size: 2573 2233 1494 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2250 2646 3699 Size: 416 53 83 399 24 833 396 1053 Cuts at: 3699 5187 6145 442 Size: 1488 958 1753 Fragments arranged by size: 1753 1488 1053 958 833 416 399 396 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2097 2342 2993 3297 3405 3459 4317 4481 Size: 858 245 651 304 108 54 858 164 Cuts at: 4481 6116 1239 Size: 1635 2579 Fragments arranged by size: 2579 1635 858 858 651 304 245 164 108 54 Bpu10I CC'TnA_GC Cuts at: 2415 3936 2415 Size: 1521 5935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2758 4091 4349 5581 6446 6744 2758 Size: 1333 258 1232 865 298 3470 Fragments arranged by size: 3470 1333 1232 865 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3631 6119 882 Size: 617 2132 2488 2219 Fragments arranged by size: 2488 2219 2132 617 BsaAI yAC'GTr Cuts at: 493 1204 5247 493 Size: 711 4043 2702 Fragments arranged by size: 4043 2702 711 BsaBI GATnn'nnATC Cuts at: 2063 7450 2063 Size: 5387 2069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2985 3976 4068 5263 Size: 53 83 186 936 1452 991 92 1195 Cuts at: 5263 5645 6280 275 Size: 382 635 1451 Fragments arranged by size: 1452 1451 1195 991 936 635 382 186 92 83 53 BsaWI w'CCGG_w Cuts at: 2244 5889 6287 6561 6708 7119 2244 Size: 3645 398 274 147 411 2581 Fragments arranged by size: 3645 2581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2340 2370 2638 2668 4199 Size: 30 165 30 937 30 268 30 1531 Cuts at: 4199 4229 4454 4484 4568 4598 1178 Size: 30 225 30 84 30 4036 Fragments arranged by size: 4036 1531 937 268 225 165 84 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2894 3812 3833 3842 3860 4466 1186 Size: 1708 918 21 9 18 606 4176 Fragments arranged by size: 4176 1708 918 606 21 18 9 BseYI C'CCAG_C Cuts at: 2475 3278 3332 3368 3440 4097 4117 4264 6659 Size: 803 54 36 72 657 20 147 2395 Cuts at: 6659 7155 2475 Size: 496 2776 Fragments arranged by size: 2776 2395 803 657 496 147 72 54 36 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 2511 2729 3980 1163 Size: 573 638 137 218 1251 4639 Fragments arranged by size: 4639 1251 638 573 218 137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4906 5667 6695 1540 Size: 275 186 2905 761 1028 2301 Fragments arranged by size: 2905 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2039 2214 3063 3668 4602 5517 5602 6673 2039 Size: 175 849 605 934 915 85 1071 2822 Fragments arranged by size: 2822 1071 934 915 849 605 175 85 BsmI GAATG_Cn' Cuts at: 4967 5060 4967 Size: 93 7363 BsmBI CGTCTCn'nnnn_ Cuts at: 3200 3914 3200 Size: 714 6742 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2039 2214 3063 3291 3668 4014 Size: 399 235 14 175 849 228 377 346 Cuts at: 4014 4023 4303 4602 4671 4775 5517 5602 6673 Size: 9 280 299 69 104 742 85 1071 Cuts at: 6673 1391 Size: 2174 Fragments arranged by size: 2174 1071 849 742 399 377 346 299 280 235 228 175 104 85 69 14 9 BspEI T'CCGG_A Cuts at: 2244 7119 2244 Size: 4875 2581 BspHI T'CATG_A Cuts at: 5345 7075 5345 Size: 1730 5726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2952 3517 3709 4623 4811 844 Size: 2108 565 192 914 188 3489 Fragments arranged by size: 3489 2108 914 565 192 188 BsrBI CCG'CTC Cuts at: 1394 1823 3365 4182 5343 1394 Size: 429 1542 817 1161 3507 Fragments arranged by size: 3507 1542 1161 817 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4132 5951 6125 65 Size: 1183 2884 1819 174 1396 Fragments arranged by size: 2884 1819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6100 6287 1700 Size: 110 4290 187 2869 Fragments arranged by size: 4290 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7456 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7456 BssSI C'ACGA_G Cuts at: 2237 2273 5516 6528 2237 Size: 36 3243 1012 3165 Fragments arranged by size: 3243 3165 1012 36 BstAPI GCAn_nnn'nTGC Cuts at: 794 2034 2521 794 Size: 1240 487 5729 Fragments arranged by size: 5729 1240 487 BstBI TT'CG_AA Cuts at: 6260 6260 Size: 7456 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7456 BstXI CCAn_nnnn'nTGG Cuts at: 2812 2812 Size: 7456 BstYI r'GATC_y Cuts at: 1972 2241 2842 3413 3607 4269 4533 4786 5153 Size: 269 601 571 194 662 264 253 367 Cuts at: 5153 5538 5555 7007 7093 7105 7451 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 662 601 571 385 367 346 269 264 253 194 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2990 3732 2990 Size: 742 6714 BtgI C'CryG_G Cuts at: 513 1065 2130 2395 2725 4308 513 Size: 552 1065 265 330 1583 3661 Fragments arranged by size: 3661 1583 1065 552 330 265 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3139 3885 4534 487 Size: 683 56 1913 746 649 3409 Fragments arranged by size: 3409 1913 746 683 649 56 BtsI GCAGTG_nn' Cuts at: 2541 3898 3950 4048 4422 4972 5766 5786 2541 Size: 1357 52 98 374 550 794 20 4211 Fragments arranged by size: 4211 1357 794 550 374 98 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7456 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7421 DraI TTT'AAA Cuts at: 4760 4760 Size: 7456 DraIII CAC_nnn'GTG Cuts at: 3956 3956 Size: 7456 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6463 809 Size: 507 5147 1802 Fragments arranged by size: 5147 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 2974 3298 Size: 54 1425 50 275 18 666 478 324 Cuts at: 3298 4903 5792 7421 8 Size: 1605 889 1629 43 Fragments arranged by size: 1629 1605 1425 889 666 478 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4903 1537 Size: 275 3091 4090 Fragments arranged by size: 4090 3091 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2957 3119 3218 3804 4160 4217 5386 1353 Size: 1604 162 99 586 356 57 1169 3423 Fragments arranged by size: 3423 1604 1169 586 356 162 99 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2165 3426 4297 6043 6417 6563 Size: 1064 138 576 1261 871 1746 374 146 Cuts at: 6563 387 Size: 1280 Fragments arranged by size: 1746 1280 1261 1064 871 576 374 146 138 EcoICRI GAG'CTC Cuts at: 3666 3666 Size: 7456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2342 2993 3021 3297 3405 3429 Size: 393 465 245 651 28 276 108 24 Cuts at: 3429 3459 4258 4317 4481 5519 6116 6903 7171 Size: 30 799 59 164 1038 597 787 268 Cuts at: 7171 1239 Size: 1524 Fragments arranged by size: 1524 1038 799 787 651 597 465 393 276 268 245 164 108 59 30 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 3650 4004 4244 3650 Size: 354 240 6862 Fragments arranged by size: 6862 354 240 EcoO109I rG'GnC_Cy Cuts at: 2320 3254 3502 3538 3589 3643 3673 3694 4019 Size: 934 248 36 51 54 30 21 325 Cuts at: 4019 4020 2320 Size: 1 5756 Fragments arranged by size: 5756 934 325 248 54 51 36 30 21 1 EcoRI G'AATT_C Cuts at: 4765 5143 4765 Size: 378 7078 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 4448 4552 4678 2101 Size: 2347 104 126 4879 Fragments arranged by size: 4879 2347 126 104 FspI TGC'GCA Cuts at: 5962 5962 Size: 7456 HaeII r_GCGC'y Cuts at: 1678 2047 2425 2485 3979 4071 6603 1678 Size: 369 378 60 1494 92 2532 2531 Fragments arranged by size: 2532 2531 1494 378 369 92 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 4199 4231 6097 Size: 32 135 32 773 32 1822 32 1866 Cuts at: 6097 6129 6171 6203 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1866 1822 773 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2346 4576 4800 5046 669 Size: 988 281 408 2230 224 246 3079 Fragments arranged by size: 3079 2230 988 408 281 246 224 HindIII A'AGCT_T Cuts at: 748 748 Size: 7456 HpaI GTT'AAC Cuts at: 5046 5046 Size: 7456 KasI G'GCGC_C Cuts at: 3975 4067 3975 Size: 92 7364 KpnI G_GTAC'C Cuts at: 4781 4781 Size: 7456 MfeI C'AATT_G Cuts at: 5055 5055 Size: 7456 MluI A'CGCG_T Cuts at: 7332 7332 Size: 7456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3126 4059 6570 6754 1305 Size: 1821 933 2511 184 2007 Fragments arranged by size: 2511 2007 1821 933 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 3300 10 Size: 54 1425 343 666 802 4166 Fragments arranged by size: 4166 1425 802 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 5414 5773 5932 518 Size: 648 1326 2922 359 159 2042 Fragments arranged by size: 2922 2042 1326 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2173 2191 2430 2581 2599 2979 3172 4310 Size: 567 18 239 151 18 380 193 1138 Cuts at: 4310 4872 5549 6697 6942 1606 Size: 562 677 1148 245 2120 Fragments arranged by size: 2120 1148 1138 677 567 562 380 245 239 193 151 18 18 NarI GG'CG_CC Cuts at: 3976 4068 3976 Size: 92 7364 NcoI C'CATG_G Cuts at: 513 1065 2395 513 Size: 552 1330 5574 Fragments arranged by size: 5574 1330 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7456 NotI GC'GGCC_GC Cuts at: 4903 4903 Size: 7456 NruI TCG'CGA Cuts at: 4553 4553 Size: 7456 NspI r_CATG'y Cuts at: 1132 1229 2765 4090 4566 4814 6359 1132 Size: 97 1536 1325 476 248 1545 2229 Fragments arranged by size: 2229 1545 1536 1325 476 248 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7456 PciI A'CATG_T Cuts at: 6355 6355 Size: 7456 PflMI CCAn_nnn'nTGG Cuts at: 1108 2131 1108 Size: 1023 6433 PfoI T'CCnGG_A Cuts at: 3313 3570 4217 3313 Size: 257 647 6552 Fragments arranged by size: 6552 647 257 PmeI GTTT'AAAC Cuts at: 4760 4760 Size: 7456 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5525 5557 7038 7070 5525 Size: 32 1481 32 5911 Fragments arranged by size: 5911 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 3502 3538 3589 3673 3694 2320 Size: 1182 36 51 84 21 6082 Fragments arranged by size: 6082 1182 84 51 36 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7456 PsiI TTA'TAA Cuts at: 5026 5026 Size: 7456 PspOMI G'GGCC_C Cuts at: 4019 4019 Size: 7456 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2987 3019 2987 Size: 32 7424 PstI C_TGCA'G Cuts at: 830 2169 2190 3027 4008 4620 4808 830 Size: 1339 21 837 981 612 188 3478 Fragments arranged by size: 3478 1339 981 837 612 188 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7456 PvuII CAG'CTG Cuts at: 2173 2191 2430 2599 3172 2173 Size: 18 239 169 573 6457 Fragments arranged by size: 6457 573 239 169 18 SacI G_AGCT'C Cuts at: 3668 3668 Size: 7456 SacII CC_GC'GG Cuts at: 4311 4311 Size: 7456 SalI G'TCGA_C Cuts at: 1936 2344 4798 1936 Size: 408 2454 4594 Fragments arranged by size: 4594 2454 408 SanDI GG'GwC_CC Cuts at: 3694 3694 Size: 7456 SapI GCTCTTCn'nnn_ Cuts at: 2957 2957 Size: 7456 SbfI CC_TGCA'GG Cuts at: 4808 4808 Size: 7456 ScaI AGT'ACT Cuts at: 5704 7416 5704 Size: 1712 5744 SexAI A'CCwGG_T Cuts at: 2436 2436 Size: 7456 SfcI C'TryA_G Cuts at: 826 1045 2165 2186 3023 3754 4004 4616 4804 Size: 219 1120 21 837 731 250 612 188 Cuts at: 4804 5939 6620 6811 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 837 731 681 612 250 219 191 188 21 SfoI GGC'GCC Cuts at: 3977 4069 3977 Size: 92 7364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7456 SmaI CCC'GGG Cuts at: 1872 3587 4783 1872 Size: 1715 1196 4545 Fragments arranged by size: 4545 1715 1196 SmlI C'TyrA_G Cuts at: 820 1017 1943 2773 4106 4364 5560 6461 6723 Size: 197 926 830 1333 258 1196 901 262 Cuts at: 6723 820 Size: 1553 Fragments arranged by size: 1553 1333 1196 926 901 830 262 258 197 SnaBI TAC'GTA Cuts at: 493 5247 493 Size: 4754 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7456 SphI G_CATG'C Cuts at: 1132 2765 4090 4566 4814 1132 Size: 1633 1325 476 248 3774 Fragments arranged by size: 3774 1633 1325 476 248 SspI AAT'ATT Cuts at: 5 52 5380 5 Size: 47 5328 2081 Fragments arranged by size: 5328 2081 47 StuI AGG'CCT Cuts at: 1517 2203 2676 1517 Size: 686 473 6297 Fragments arranged by size: 6297 686 473 StyI C'CwwG_G Cuts at: 513 1065 2395 2958 3237 3639 4245 4380 4521 Size: 552 1330 563 279 402 606 135 141 Cuts at: 4521 4605 4895 513 Size: 84 290 3074 Fragments arranged by size: 3074 1330 606 563 552 402 290 279 141 135 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 3292 3346 5483 5500 5653 5838 Size: 1255 828 884 54 2137 17 153 185 Cuts at: 5838 6283 325 Size: 445 1498 Fragments arranged by size: 2137 1498 1255 884 828 445 185 153 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5702 7414 96 Size: 274 80 33 51 1443 3725 1712 138 Fragments arranged by size: 3725 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2372 4133 4165 7416 7448 2340 Size: 32 1761 32 3251 32 2348 Fragments arranged by size: 3251 2348 1761 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7456 XbaI T'CTAG_A Cuts at: 2839 4792 2839 Size: 1953 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7456 XmaI C'CCGG_G Cuts at: 1870 3585 4781 1870 Size: 1715 1196 4545 Fragments arranged by size: 4545 1715 1196 XmnI GAAnn'nnTTC Cuts at: 2012 4337 5585 2012 Size: 2325 1248 3883 Fragments arranged by size: 3883 2325 1248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2986 5264 276 Size: 53 83 186 936 1452 2278 2468 Fragments arranged by size: 2468 2278 1452 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PmlI PspXI RsrII SfiI SrfI SwaI XcmI