(Linear) (Six-base) MAPSORT of: KIEE5827.seq Check: 9382 from: 1 to: 2751 KIEE5827.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1704 2751 Size: 1704 1047 AatII G_ACGT'C Cuts at: 0 983 2751 Size: 983 1768 AccI GT'mk_AC Cuts at: 0 340 2751 Size: 340 2411 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1016 1424 2253 2751 Size: 1016 408 829 498 Fragments arranged by size: 1016 829 498 408 AfeI AGC'GCT Cuts at: 0 478 2751 Size: 478 2273 AhdI GACnn_n'nnGTC Cuts at: 0 354 2751 Size: 354 2397 AleI CACnn'nnGTG Cuts at: 0 487 2751 Size: 487 2264 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1462 2014 2751 Size: 1462 552 737 Fragments arranged by size: 1462 737 552 AlwNI CAG_nnn'CTG Cuts at: 0 168 1684 2751 Size: 168 1516 1067 Fragments arranged by size: 1516 1067 168 ApaI G_GGCC'C Cuts at: 0 2018 2751 Size: 2018 733 ApoI r'AATT_y Cuts at: 0 1045 2751 Size: 1045 1706 AvaI C'yCGr_G Cuts at: 0 63 1580 1782 2009 2751 Size: 63 1517 202 227 742 Fragments arranged by size: 1517 742 227 202 63 BamHI G'GATC_C Cuts at: 0 1408 2751 Size: 1408 1343 BanI G'GyrC_C Cuts at: 0 778 1133 1255 1970 2062 2751 Size: 778 355 122 715 92 689 Fragments arranged by size: 778 715 689 355 122 92 BanII G_rGCy'C Cuts at: 0 20 1286 1663 2009 2018 2298 2666 2751 Size: 20 1266 377 346 9 280 368 85 Fragments arranged by size: 1266 377 368 346 280 85 20 9 BbeI G_GCGC'C Cuts at: 0 1974 2066 2751 Size: 1974 92 685 Fragments arranged by size: 1974 685 92 BbsI GAAGACnn'nnnn_ Cuts at: 0 703 1043 2751 Size: 703 340 1708 Fragments arranged by size: 1708 703 340 BbvCI CC'TCA_GC Cuts at: 0 1931 2751 Size: 1931 820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1893 1927 2000 2034 2751 Size: 1893 34 73 34 717 Fragments arranged by size: 1893 717 73 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 612 2751 Size: 612 2139 BglI GCCn_nnn'nGGC Cuts at: 0 2013 2751 Size: 2013 738 BglII A'GATC_T Cuts at: 0 837 1602 2528 2751 Size: 837 765 926 223 Fragments arranged by size: 926 837 765 223 Bme1580I G_kGCm'C Cuts at: 0 2018 2751 Size: 2018 733 BmrI ACTGGGnnnn_n' Cuts at: 0 245 641 1694 2751 Size: 245 396 1053 1057 Fragments arranged by size: 1057 1053 396 245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 92 337 988 1292 1400 1454 2312 2476 Size: 92 245 651 304 108 54 858 164 Cuts at: 2476 2751 Size: 275 Fragments arranged by size: 858 651 304 275 245 164 108 92 54 Bpu10I CC'TnA_GC Cuts at: 0 410 1931 2751 Size: 410 1521 820 Fragments arranged by size: 1521 820 410 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 753 2086 2344 2751 Size: 753 1333 258 407 Fragments arranged by size: 1333 753 407 258 BsaI GGTCTCn'nnnn_ Cuts at: 0 1626 2751 Size: 1626 1125 BsaBI GATnn'nnATC Cuts at: 0 58 2751 Size: 58 2693 BsaHI Gr'CG_yC Cuts at: 0 980 1971 2063 2751 Size: 980 991 92 688 Fragments arranged by size: 991 980 688 92 BsaWI w'CCGG_w Cuts at: 0 239 2751 Size: 239 2512 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 335 365 633 663 2194 2224 2449 2479 Size: 335 30 268 30 1531 30 225 30 Cuts at: 2479 2563 2593 2751 Size: 84 30 158 Fragments arranged by size: 1531 335 268 225 158 84 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 889 1807 1828 1837 1855 2461 2751 Size: 889 918 21 9 18 606 290 Fragments arranged by size: 918 889 606 290 21 18 9 BseYI C'CCAG_C Cuts at: 0 470 1273 1327 1363 1435 2092 2112 2259 Size: 470 803 54 36 72 657 20 147 Cuts at: 2259 2751 Size: 492 Fragments arranged by size: 803 657 492 470 147 72 54 36 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 369 506 724 1975 2751 Size: 369 137 218 1251 776 Fragments arranged by size: 1251 776 369 218 137 BsiHKAI G_wGCw'C Cuts at: 0 34 209 1058 1663 2597 2751 Size: 34 175 849 605 934 154 Fragments arranged by size: 934 849 605 175 154 34 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1195 1909 2751 Size: 1195 714 842 Fragments arranged by size: 1195 842 714 Bsp1286I G_dGCh'C Cuts at: 0 20 34 209 1058 1286 1663 2009 2018 Size: 20 14 175 849 228 377 346 9 Cuts at: 2018 2298 2597 2666 2751 Size: 280 299 69 85 Fragments arranged by size: 849 377 346 299 280 228 175 85 69 20 14 9 BspEI T'CCGG_A Cuts at: 0 239 2751 Size: 239 2512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 947 1512 1704 2618 2751 Size: 947 565 192 914 133 Fragments arranged by size: 947 914 565 192 133 BsrBI CCG'CTC Cuts at: 0 1360 2177 2751 Size: 1360 817 574 Fragments arranged by size: 1360 817 574 BsrDI GCAATG_nn' Cuts at: 0 2127 2751 Size: 2127 624 BssSI C'ACGA_G Cuts at: 0 232 268 2751 Size: 232 36 2483 Fragments arranged by size: 2483 232 36 BstAPI GCAn_nnn'nTGC Cuts at: 0 29 516 2751 Size: 29 487 2235 Fragments arranged by size: 2235 487 29 BstXI CCAn_nnnn'nTGG Cuts at: 0 807 2751 Size: 807 1944 BstYI r'GATC_y Cuts at: 0 236 837 1408 1602 2264 2528 2751 Size: 236 601 571 194 662 264 223 Fragments arranged by size: 662 601 571 264 236 223 194 Bsu36I CC'TnA_GG Cuts at: 0 985 1727 2751 Size: 985 742 1024 Fragments arranged by size: 1024 985 742 BtgI C'CryG_G Cuts at: 0 125 390 720 2303 2751 Size: 125 265 330 1583 448 Fragments arranged by size: 1583 448 330 265 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1134 1880 2529 2751 Size: 1134 746 649 222 Fragments arranged by size: 1134 746 649 222 BtsI GCAGTG_nn' Cuts at: 0 536 1893 1945 2043 2417 2751 Size: 536 1357 52 98 374 334 Fragments arranged by size: 1357 536 374 334 98 52 DraIII CAC_nnn'GTG Cuts at: 0 1951 2751 Size: 1951 800 EaeI y'GGCC_r Cuts at: 0 491 969 1293 2751 Size: 491 478 324 1458 Fragments arranged by size: 1458 491 478 324 EarI CTCTTCn'nnn_ Cuts at: 0 952 1114 1213 1799 2155 2212 2751 Size: 952 162 99 586 356 57 539 Fragments arranged by size: 952 586 539 356 162 99 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 160 1421 2292 2751 Size: 160 1261 871 459 Fragments arranged by size: 1261 871 459 160 EcoICRI GAG'CTC Cuts at: 0 1661 2751 Size: 1661 1090 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 92 337 988 1016 1292 1400 1424 1454 Size: 92 245 651 28 276 108 24 30 Cuts at: 1454 2253 2312 2476 2751 Size: 799 59 164 275 Fragments arranged by size: 799 651 276 275 245 164 108 92 59 30 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1645 1999 2239 2751 Size: 1645 354 240 512 Fragments arranged by size: 1645 512 354 240 EcoO109I rG'GnC_Cy Cuts at: 0 315 1249 1497 1533 1584 1638 1668 1689 Size: 315 934 248 36 51 54 30 21 Cuts at: 1689 2014 2015 2751 Size: 325 1 736 Fragments arranged by size: 934 736 325 315 248 54 51 36 30 21 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 96 2443 2547 2673 2751 Size: 96 2347 104 126 78 Fragments arranged by size: 2347 126 104 96 78 HaeII r_GCGC'y Cuts at: 0 42 420 480 1974 2066 2751 Size: 42 378 60 1494 92 685 Fragments arranged by size: 1494 685 378 92 60 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 2194 2226 2751 Size: 340 32 1822 32 525 Fragments arranged by size: 1822 525 340 32 32 HincII GTy'rAC Cuts at: 0 341 2571 2751 Size: 341 2230 180 Fragments arranged by size: 2230 341 180 KasI G'GCGC_C Cuts at: 0 1970 2062 2751 Size: 1970 92 689 Fragments arranged by size: 1970 689 92 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1121 2054 2751 Size: 1121 933 697 Fragments arranged by size: 1121 933 697 MscI TGG'CCA Cuts at: 0 493 1295 2751 Size: 493 802 1456 Fragments arranged by size: 1456 802 493 MslI CAynn'nnrTG Cuts at: 0 487 2751 Size: 487 2264 MspA1I CmG'CkG Cuts at: 0 168 186 425 576 594 974 1167 2305 Size: 168 18 239 151 18 380 193 1138 Cuts at: 2305 2751 Size: 446 Fragments arranged by size: 1138 446 380 239 193 168 151 18 18 NarI GG'CG_CC Cuts at: 0 1971 2063 2751 Size: 1971 92 688 Fragments arranged by size: 1971 688 92 NcoI C'CATG_G Cuts at: 0 390 2751 Size: 390 2361 NruI TCG'CGA Cuts at: 0 2548 2751 Size: 2548 203 NspI r_CATG'y Cuts at: 0 760 2085 2561 2751 Size: 760 1325 476 190 Fragments arranged by size: 1325 760 476 190 PflMI CCAn_nnn'nTGG Cuts at: 0 126 2751 Size: 126 2625 PfoI T'CCnGG_A Cuts at: 0 1308 1565 2212 2751 Size: 1308 257 647 539 Fragments arranged by size: 1308 647 539 257 PpuMI rG'GwC_Cy Cuts at: 0 315 1497 1533 1584 1668 1689 2751 Size: 315 1182 36 51 84 21 1062 Fragments arranged by size: 1182 1062 315 84 51 36 21 PspOMI G'GGCC_C Cuts at: 0 2014 2751 Size: 2014 737 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 982 1014 2751 Size: 982 32 1737 Fragments arranged by size: 1737 982 32 PstI C_TGCA'G Cuts at: 0 164 185 1022 2003 2615 2751 Size: 164 21 837 981 612 136 Fragments arranged by size: 981 837 612 164 136 21 PvuII CAG'CTG Cuts at: 0 168 186 425 594 1167 2751 Size: 168 18 239 169 573 1584 Fragments arranged by size: 1584 573 239 169 168 18 SacI G_AGCT'C Cuts at: 0 1663 2751 Size: 1663 1088 SacII CC_GC'GG Cuts at: 0 2306 2751 Size: 2306 445 SalI G'TCGA_C Cuts at: 0 339 2751 Size: 339 2412 SanDI GG'GwC_CC Cuts at: 0 1689 2751 Size: 1689 1062 SapI GCTCTTCn'nnn_ Cuts at: 0 952 2751 Size: 952 1799 SexAI A'CCwGG_T Cuts at: 0 431 2751 Size: 431 2320 SfcI C'TryA_G Cuts at: 0 160 181 1018 1749 1999 2611 2751 Size: 160 21 837 731 250 612 140 Fragments arranged by size: 837 731 612 250 160 140 21 SfoI GGC'GCC Cuts at: 0 1972 2064 2751 Size: 1972 92 687 Fragments arranged by size: 1972 687 92 SmaI CCC'GGG Cuts at: 0 1582 2751 Size: 1582 1169 SmlI C'TyrA_G Cuts at: 0 768 2101 2359 2751 Size: 768 1333 258 392 Fragments arranged by size: 1333 768 392 258 SphI G_CATG'C Cuts at: 0 760 2085 2561 2751 Size: 760 1325 476 190 Fragments arranged by size: 1325 760 476 190 StuI AGG'CCT Cuts at: 0 198 671 2751 Size: 198 473 2080 Fragments arranged by size: 2080 473 198 StyI C'CwwG_G Cuts at: 0 390 953 1232 1634 2240 2375 2516 2600 Size: 390 563 279 402 606 135 141 84 Cuts at: 2600 2751 Size: 151 Fragments arranged by size: 606 563 402 390 279 151 141 135 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 403 1287 1341 2751 Size: 403 884 54 1410 Fragments arranged by size: 1410 884 403 54 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 335 367 2128 2160 2751 Size: 335 32 1761 32 591 Fragments arranged by size: 1761 591 335 32 32 XbaI T'CTAG_A Cuts at: 0 834 2751 Size: 834 1917 XmaI C'CCGG_G Cuts at: 0 1580 2751 Size: 1580 1171 XmnI GAAnn'nnTTC Cuts at: 0 7 2332 2751 Size: 7 2325 419 Fragments arranged by size: 2325 419 7 ZraI GAC'GTC Cuts at: 0 981 2751 Size: 981 1770 Enzymes that do cut: AarI AatII AccI AcuI AfeI AhdI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NruI NspI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SacII SalI SanDI SapI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TstI XbaI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsiEI BsiWI BsmI BspHI BsrFI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XcmI XhoI