################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccII 190 CGCG CG^CG AcsI 626 GAATTC [GA]^AATT[CT] AlwNI 640 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 626 GAATTC [GA]^AATT[CT] BamHI 388 GGATCC G^GATCC BcnI 422 CCCGG CC^[GC]GG BglII 43 AGATCT A^GATCT BlpI 260 GCTCAGC GC^T[ACGT]AGC Bpu1102I 260 GCTCAGC GC^T[ACGT]AGC BsgI 138 GTGCAG GTGCAG(16/14) BstUI 190 CGCG CG^CG BstXI 323 CCAAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 256 GCTCGC GC[ACGT]^[ACGT]GC CelII 260 GCTCAGC GC^T[ACGT]AGC DraIII 321 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 626 GAATTC G^AATTC EcoT14I 708 CCAAGG C^C[AT][AT]GG HincII 175 GTCAAC GT[CT]^[GA]AC HindII 175 GTCAAC GT[CT]^[GA]AC MaeIII 602 GTGAC ^GT[ACGT]AC MspA1I 64 CAGCTG C[AC]G^C[GT]G MvnI 190 CGCG CG^CG NciI 422 CCCGG CC^[GC]GG NspI 507 ACATGC [GA]CATG^[CT] PvuII 64 CAGCTG CAG^CTG SapI 414 GAAGAGC GCTCTTC(1/4) SmlI 721 CTCAAG C^T[CT][GA]AG StyI 708 CCAAGG C^C[AT][AT]GG TfiI 296 GAATC G^A[AT]TC Tsp45I 602 GTGAC ^GT[GC]AC Tsp509I 627 AATT ^AATT XcmI 323 CCAAGTGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 494 TCCGGA T^CCGGA 548 TCCGGA AlwI 389 GATCC GGATC(4/5) 388 GGATC ApaI 122 GGGCCC GGGCC^C 196 GGGCCC AspHI 371 GAGCAC G[AT]GC[AT]^C 417 GAGCAC AvaII 26 GGACC G^G[AT]CC 131 GGTCC BanI 280 GGTGCC G^G[CT][GA]CC 319 GGCACC BfaI 607 CTAG C^TAG 658 CTAG BglI 311 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 667 GCCATTATGGC BsaI 109 GAGACC GGTCTC(1/5) 337 GAGACC BsaWI 494 TCCGGA [AT]^CCGG[AT] 548 TCCGGA BseAI 494 TCCGGA T^CCGGA 548 TCCGGA BseRI 561 CTCCTC GAGGAG(10/8) 368 GAGGAG BsiHKAI 371 GAGCAC G[AT]GC[AT]^C 417 GAGCAC BsmAI 109 GAGAC GTCTC(1/5) 337 GAGAC BspEI 494 TCCGGA T^CCGGA 548 TCCGGA BstF5I 497 GGATG GGATG(2/0) 726 GGATG BstYI 43 AGATCT [GA]^GATC[CT] 388 GGATCC CfoI 36 GCGC GCG^C 191 GCGC FauI 534 GCGGG CCCGC(4/6) 622 GCGGG FokI 497 GGATG GGATG(9/13) 726 GGATG HhaI 36 GCGC GCG^C 191 GCGC HinP1I 36 GCGC G^CGC 191 GCGC HphI 345 GGTGA GGTGA(8/7) 480 TCACC MaeI 607 CTAG C^TAG 658 CTAG MflI 43 AGATCT [GA]^GATC[CT] 388 GGATCC MroI 494 TCCGGA T^CCGGA 548 TCCGGA NlaIII 21 CATG CATG^ 508 CATG PleI 304 GAGTC GAGTC(4/5) 592 GAGTC PpuMI 25 GGGACCT [GA]G^G[AT]CC[CT] 130 GGGTCCC PstI 85 CTGCAG CTGCA^G 251 CTGCAG SfaNI 477 GCATC GCATC(5/9) 633 GCATC TspRI 384 CAGTG CA[GC]TG[ACGT][ACGT]^ 600 CAGTG XhoII 43 AGATCT [GA]^GATC[CT] 388 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI Ksp632I MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfcI TseI