Restriction map for
pFN21AE6653
(Circular) (Six-base) MAPSORT of: pFN21AE6653.seq Check: 9667 from: 1 to: 5449
pFN21AE6653 5449 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2105 1184
Size: 921 4528
AlwNI CAG_nnn'CTG
Cuts at: 2650 3192 4764 2650
Size: 542 1572 3335
Fragments arranged by size:
3335 1572 542
ApaI G_GGCC'C
Cuts at: 2131 2205 2131
Size: 74 5375
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2631 2758 2984 3136 5245 1493
Size: 1138 127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1138 226 152 127
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2774 1870
Size: 73 831 4545
Fragments arranged by size:
4545 831 73
BamHI G'GATC_C
Cuts at: 2393 2779 3146 2393
Size: 386 367 4696
Fragments arranged by size:
4696 386 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2285 2324 2770 4225 618
Size: 325 217 627 498 39 446 1455 1842
Fragments arranged by size:
1842 1455 627 498 446 325 217 39
BanII G_rGCy'C
Cuts at: 1391 2131 2205 2510 2561 2580 2768 1391
Size: 740 74 305 51 19 188 4072
Fragments arranged by size:
4072 740 305 188 74 51 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4478
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2322 2678 4060
Size: 107 122 71 631 605 650 356 1382
Cuts at: 4060 136
Size: 1525
Fragments arranged by size:
1525 1382 650 631 605 356 122 107
71
BglII A'GATC_T
Cuts at: 2048 5444 2048
Size: 3396 2053
BlpI GC'TnA_GC
Cuts at: 2266 2866 2266
Size: 600 4849
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2205 3510 4666 1790
Size: 341 74 1305 1156 2573
Fragments arranged by size:
2573 1305 1156 341 74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2093 2234 2457 2627 4109 1239
Size: 800 54 141 223 170 1482 2579
Fragments arranged by size:
2579 1482 800 223 170 141 54
Bpu10I CC'TnA_GC
Cuts at: 2411 2411
Size: 5449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 3574 4439 4737 2711
Size: 863 865 298 3423
Fragments arranged by size:
3423 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2336 4112 882
Size: 617 609 228 1776 2219
Fragments arranged by size:
2219 1776 617 609 228
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 2553 3882 4280 4554 4701 5112 2499
Size: 54 1329 398 274 147 411 2836
Fragments arranged by size:
2836 1329 411 398 274 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5224
Fragments arranged by size:
5224 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 2557 1186
Size: 1202 169 4078
Fragments arranged by size:
4078 1202 169
BseYI C'CCAG_C
Cuts at: 2591 4652 5148 2591
Size: 2061 496 2892
Fragments arranged by size:
2892 2061 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2164 1163
Size: 573 428 4448
Fragments arranged by size:
4448 573 428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2380 2426 3510 3595 4666 2380
Size: 46 1084 85 1071 3163
Fragments arranged by size:
3163 1084 1071 85 46
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2205 2380 2426 2510 2561 2580
Size: 399 341 74 175 46 84 51 19
Cuts at: 2580 2768 3510 3595 4666 1391
Size: 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 188 175 85
84 74 51 46 19
BspEI T'CCGG_A
Cuts at: 2499 2553 5112 2499
Size: 54 2559 2836
Fragments arranged by size:
2836 2559 54
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2335
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2048 2393 2779 3146 3531 3548 5000 5086
Size: 76 345 386 367 385 17 1452 86
Cuts at: 5086 5098 5444 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 386 385 367 346 345 86
76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3958
Fragments arranged by size:
3958 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2395 2965 3759 3779 2395
Size: 570 794 20 4065
Fragments arranged by size:
4065 794 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DraIII CAC_nnn'GTG
Cuts at: 2331 2331
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3785 5414
Size: 54 1425 50 275 18 1066 889 1629
Cuts at: 5414 8
Size: 43
Fragments arranged by size:
1629 1425 1066 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2363 2414 2679 3379 1353
Size: 1010 51 265 700 3423
Fragments arranged by size:
3423 1010 700 265 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2380 2696 4036 4410 4556 387
Size: 1064 138 791 316 1340 374 146 1280
Fragments arranged by size:
1340 1280 1064 791 374 316 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2093 2234 2457 2627 3512 4109
Size: 393 407 54 141 223 170 885 597
Cuts at: 4109 4896 5164 1239
Size: 787 268 1524
Fragments arranged by size:
1524 885 787 597 407 393 268 223
170 141 54
EcoO109I rG'GnC_Cy
Cuts at: 2031 2127 2128 2136 2201 2202 2245 2031
Size: 96 1 8 65 1 43 5235
Fragments arranged by size:
5235 96 65 43 8 1 1
EcoRI G'AATT_C
Cuts at: 2631 2758 3136 2631
Size: 127 378 4944
Fragments arranged by size:
4944 378 127
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 4596 1678
Size: 2918 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4090 4122 4164 4196 1373
Size: 32 135 32 2518 32 42 32 2626
Fragments arranged by size:
2626 2518 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2793 3039 669
Size: 988 281 244 611 246 3079
Fragments arranged by size:
3079 988 611 281 246 244
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4563 4747 1305
Size: 3258 184 2007
Fragments arranged by size:
3258 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3627
Fragments arranged by size:
3627 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2865 3542 4690 4935 1606
Size: 465 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 465 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3958
Fragments arranged by size:
3958 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2516 2807 4352 1132
Size: 97 1287 291 1545 2229
Fragments arranged by size:
2229 1545 1287 291 97
PasI CC'CwG_GG
Cuts at: 1348 2131 2469 1348
Size: 783 338 4328
Fragments arranged by size:
4328 783 338
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PfoI T'CCnGG_A
Cuts at: 2207 2304 2207
Size: 97 5352
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2031 2136 2031
Size: 105 5344
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2127 2201 2127
Size: 74 5375
PstI C_TGCA'G
Cuts at: 830 2094 2260 2801 830
Size: 1264 166 541 3478
Fragments arranged by size:
3478 1264 541 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
PvuII CAG'CTG
Cuts at: 2071 2071
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SanDI GG'GwC_CC
Cuts at: 2136 2136
Size: 5449
SapI GCTCTTCn'nnn_
Cuts at: 2414 2414
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2090 2121 2256 2797 3932 4613 4804
Size: 219 1045 31 135 541 1135 681 191
Cuts at: 4804 826
Size: 1471
Fragments arranged by size:
1471 1135 1045 681 541 219 191 135
31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 3553 4454 4716 820
Size: 197 926 783 827 901 262 1553
Fragments arranged by size:
1553 926 901 827 783 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5449
StyI C'CwwG_G
Cuts at: 513 1065 2004 2713 2888 513
Size: 552 939 709 175 3074
Fragments arranged by size:
3074 939 709 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 2535 3476 3493 3646 3831 4276
Size: 1255 693 262 941 17 153 185 445
Cuts at: 4276 325
Size: 1498
Fragments arranged by size:
1498 1255 941 693 445 262 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3695 5407 96
Size: 274 80 33 51 1443 1718 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5409 5441 5409
Size: 32 5417
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2335
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SexAI SfiI SfoI SrfI SwaI