(Linear) (Six-base) MAPSORT of: KIEE6653.seq Check: 6030 from: 1 to: 744 KIEE6653.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 100 744 Size: 100 644 AlwNI CAG_nnn'CTG Cuts at: 0 645 744 Size: 645 99 ApaI G_GGCC'C Cuts at: 0 126 200 744 Size: 126 74 544 Fragments arranged by size: 544 126 74 ApoI r'AATT_y Cuts at: 0 626 744 Size: 626 118 BamHI G'GATC_C Cuts at: 0 388 744 Size: 388 356 BanI G'GyrC_C Cuts at: 0 280 319 744 Size: 280 39 425 Fragments arranged by size: 425 280 39 BanII G_rGCy'C Cuts at: 0 126 200 505 556 575 744 Size: 126 74 305 51 19 169 Fragments arranged by size: 305 169 126 74 51 19 BglI GCCn_nnn'nGGC Cuts at: 0 317 673 744 Size: 317 356 71 Fragments arranged by size: 356 317 71 BglII A'GATC_T Cuts at: 0 43 744 Size: 43 701 BlpI GC'TnA_GC Cuts at: 0 261 744 Size: 261 483 Bme1580I G_kGCm'C Cuts at: 0 126 200 744 Size: 126 74 544 Fragments arranged by size: 544 126 74 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 88 229 452 622 744 Size: 34 54 141 223 170 122 Fragments arranged by size: 223 170 141 122 54 34 Bpu10I CC'TnA_GC Cuts at: 0 406 744 Size: 406 338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 744 Size: 706 38 BsaI GGTCTCn'nnnn_ Cuts at: 0 103 331 744 Size: 103 228 413 Fragments arranged by size: 413 228 103 BsaWI w'CCGG_w Cuts at: 0 494 548 744 Size: 494 54 196 Fragments arranged by size: 494 196 54 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 383 552 744 Size: 383 169 192 Fragments arranged by size: 383 192 169 BseYI C'CCAG_C Cuts at: 0 586 744 Size: 586 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 744 Size: 159 585 BsiHKAI G_wGCw'C Cuts at: 0 375 421 744 Size: 375 46 323 Fragments arranged by size: 375 323 46 Bsp1286I G_dGCh'C Cuts at: 0 126 200 375 421 505 556 575 744 Size: 126 74 175 46 84 51 19 169 Fragments arranged by size: 175 169 126 84 74 51 46 19 BspEI T'CCGG_A Cuts at: 0 494 548 744 Size: 494 54 196 Fragments arranged by size: 494 196 54 BstXI CCAn_nnnn'nTGG Cuts at: 0 330 744 Size: 330 414 BstYI r'GATC_y Cuts at: 0 43 388 744 Size: 43 345 356 Fragments arranged by size: 356 345 43 BtsI GCAGTG_nn' Cuts at: 0 390 744 Size: 390 354 DraIII CAC_nnn'GTG Cuts at: 0 326 744 Size: 326 418 EarI CTCTTCn'nnn_ Cuts at: 0 358 409 674 744 Size: 358 51 265 70 Fragments arranged by size: 358 265 70 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 375 691 744 Size: 375 316 53 Fragments arranged by size: 375 316 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 88 229 452 622 744 Size: 34 54 141 223 170 122 Fragments arranged by size: 223 170 141 122 54 34 EcoO109I rG'GnC_Cy Cuts at: 0 26 122 123 131 196 197 240 744 Size: 26 96 1 8 65 1 43 504 Fragments arranged by size: 504 96 65 43 26 8 1 1 EcoRI G'AATT_C Cuts at: 0 626 744 Size: 626 118 HincII GTy'rAC Cuts at: 0 177 744 Size: 177 567 MspA1I CmG'CkG Cuts at: 0 66 744 Size: 66 678 NspI r_CATG'y Cuts at: 0 511 744 Size: 511 233 PasI CC'CwG_GG Cuts at: 0 126 464 744 Size: 126 338 280 Fragments arranged by size: 338 280 126 PfoI T'CCnGG_A Cuts at: 0 202 299 744 Size: 202 97 445 Fragments arranged by size: 445 202 97 PpuMI rG'GwC_Cy Cuts at: 0 26 131 744 Size: 26 105 613 Fragments arranged by size: 613 105 26 PspOMI G'GGCC_C Cuts at: 0 122 196 744 Size: 122 74 548 Fragments arranged by size: 548 122 74 PstI C_TGCA'G Cuts at: 0 89 255 744 Size: 89 166 489 Fragments arranged by size: 489 166 89 PvuII CAG'CTG Cuts at: 0 66 744 Size: 66 678 SanDI GG'GwC_CC Cuts at: 0 131 744 Size: 131 613 SapI GCTCTTCn'nnn_ Cuts at: 0 409 744 Size: 409 335 SfcI C'TryA_G Cuts at: 0 85 116 251 744 Size: 85 31 135 493 Fragments arranged by size: 493 135 85 31 SmlI C'TyrA_G Cuts at: 0 721 744 Size: 721 23 StyI C'CwwG_G Cuts at: 0 708 744 Size: 708 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 268 530 744 Size: 268 262 214 Fragments arranged by size: 268 262 214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 330 744 Size: 330 414 Enzymes that do cut: AlfI AlwNI ApaI ApoI BamHI BanI BanII BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BstXI BstYI BtsI DraIII EarI EciI Eco57MI EcoO109I EcoRI HincII MspA1I NspI PasI PfoI PpuMI PspOMI PstI PvuII SanDI SapI SfcI SmlI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI