################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbeI BbsI BcgI BclI BfrI BglII BlnI BpuAI BsaWI BseAI BsiWI BspDI BspEI BspLU11I Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KasI KpnI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AhdI 568 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1093 GTGCAC G^TGCAC AocI 113 CCTCAGG CC^T[ACGT]AGG ApaI 1384 GGGCCC GGGCC^C ApaLI 1093 GTGCAC G^TGCAC Asp700I 813 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 568 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 209 TGGCCA TGG^CCA BbrPI 205 CACGTG CAC^GTG BbvCI 177 GCTGAGG CCTCAGC(-5/-2) BciVI 1229 GTATCC GTATCC(6/5) BfaI 370 CTAG C^TAG BlpI 1509 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1509 GCTGAGC GC^T[ACGT]AGC BsaAI 205 CACGTG [CT]AC^GT[GA] BsaBI 357 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 598 GAGACC GGTCTC(1/5) BsgI 973 CTGCAC GTGCAG(16/14) BsiEI 1466 CGGCCG CG[GA][CT]^CG BsmBI 1087 GAGACG CGTCTC(1/5) BsmFI 1347 GGGAC GGGAC(10/14) BsmI 1169 GCATTC GAATGC(1/-1) Bsp1407I 1040 TGTACA T^GTACA BspHI 680 TCATGA T^CATGA BsrDI 1242 CATTGC GCAATG(2/0) BsrGI 1040 TGTACA T^GTACA BssHII 1450 GCGCGC G^CGCGC BstEII 403 GGTAACC G^GT[ACGT]ACC BstPI 403 GGTAACC G^GT[ACGT]ACC Bsu36I 113 CCTCAGG CC^T[ACGT]AGG CelII 1509 GCTGAGC GC^T[ACGT]AGC EagI 1466 CGGCCG C^GGCCG Eam1105I 568 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1466 CGGCCG C^GGCCG Eco52I 1466 CGGCCG C^GGCCG Eco81I 113 CCTCAGG CC^T[ACGT]AGG EcoO65I 403 GGTAACC G^GT[ACGT]ACC HaeII 419 AGCGCC [GA]GCGC^[CT] HindIII 76 AAGCTT A^AGCTT HinfI 1066 GAGTC G^A[ACGT]TC KspI 603 CCGCGG CCGC^GG MaeI 370 CTAG C^TAG MamI 357 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 209 TGGCCA TGG^CCA MscI 209 TGGCCA TGG^CCA MslI 858 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 499 GCCGGC GCC^GGC NgoMIV 499 GCCGGC G^CCGGC NspI 953 GCATGT [GA]CATG^[CT] PleI 1066 GAGTC GAGTC(4/5) PmaCI 205 CACGTG CAC^GTG PmlI 205 CACGTG CAC^GTG PvuII 485 CAGCTG CAG^CTG RcaI 680 TCATGA T^CATGA SacII 603 CCGCGG CCGC^GG ScaI 581 AGTACT AGT^ACT SexAI 737 ACCTGGT A^CC[AT]GGT SfcI 219 CTACAG C^T[GA][CT]AG SmlI 1537 CTCAAG C^T[CT][GA]AG SspBI 1040 TGTACA T^GTACA StuI 87 AGGCCT AGG^CCT TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 813 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AatII 573 GACGTC GACGT^C 847 GACGTC AcyI 573 GACGTC G[GA]^CG[CT]C 847 GACGTC AlwI 546 GGATC GGATC(4/5) 1154 GGATC AvaI 304 CCCGAG C^[CT]CG[GA]G 1027 CCCGAG BanI 496 GGTGCC G^G[CT][GA]CC 1405 GGTGCC BsaHI 573 GACGTC G[GA]^CG[CT]C 847 GACGTC BseRI 781 GAGGAG GAGGAG(10/8) 1030 GAGGAG BsoBI 304 CCCGAG C^[CT]CG[GA]G 1027 CCCGAG BsrBI 1441 CCGCTC CCGCTC(-3/-3) 519 GAGCGG BsrFI 499 GCCGGC [GA]^CCGG[CT] 587 ACCGGC BssSI 39 CACGAG CACGAG(-5/-1) 289 CACGAG BstYI 546 GGATCT [GA]^GATC[CT] 1154 GGATCT Cfr10I 499 GCCGGC [GA]^CCGG[CT] 587 ACCGGC DsaI 603 CCGCGG C^C[GA][CT]GG 1427 CCACGG EarI 903 GAAGAG CTCTTC(1/4) 1302 GAAGAG Hin1I 573 GACGTC G[GA]^CG[CT]C 847 GACGTC Ksp632I 903 GAAGAG CTCTTC(1/4) 1302 GAAGAG MflI 546 GGATCT [GA]^GATC[CT] 1154 GGATCT PpuMI 214 AGGACCT [GA]G^G[AT]CC[CT] 1347 GGGACCC XhoII 546 GGATCT [GA]^GATC[CT] 1154 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TthHB8I