(Circular) (Six-base) MAPSORT of: pFN21AE5661.seq Check: 7065 from: 1 to: 6250 pFN21AE5661 6250 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2460 2460 Size: 6250 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2582 2856 4060 278 Size: 53 83 186 936 1046 274 1204 2468 Fragments arranged by size: 2468 1204 1046 936 274 186 83 53 AccI GT'mk_AC Cuts at: 1937 3593 1937 Size: 1656 4594 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2927 4313 5697 5965 1632 Size: 499 796 1386 1384 268 1917 Fragments arranged by size: 1917 1386 1384 796 499 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 5149 6126 1203 Size: 3946 977 1327 Fragments arranged by size: 3946 1327 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 2578 4979 2578 Size: 2401 3849 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 2468 1184 Size: 1089 195 4966 Fragments arranged by size: 4966 1089 195 AlwNI CAG_nnn'CTG Cuts at: 2386 2428 2548 3993 5565 2386 Size: 42 120 1445 1572 3071 Fragments arranged by size: 3071 1572 1445 120 42 ApaI G_GGCC'C Cuts at: 3393 3393 Size: 6250 ApaLI G'TGCA_C Cuts at: 3098 4307 5463 3098 Size: 1209 1156 3885 Fragments arranged by size: 3885 1209 1156 ApoI r'AATT_y Cuts at: 1493 2563 3082 3131 3559 3785 3937 6046 1493 Size: 1070 519 49 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 1070 519 428 226 152 49 AseI AT'TA_AT Cuts at: 160 1032 4804 160 Size: 872 3772 1606 Fragments arranged by size: 3772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 2309 3032 3575 1870 Size: 73 366 723 543 4545 Fragments arranged by size: 4545 723 543 366 73 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2501 3410 3571 5026 618 Size: 325 217 627 714 909 161 1455 1842 Fragments arranged by size: 1842 1455 909 714 627 325 217 161 BanII G_rGCy'C Cuts at: 1391 2310 3393 3441 3569 1391 Size: 919 1083 48 128 4072 Fragments arranged by size: 4072 1083 919 128 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5279 BbvCI CC'TCA_GC Cuts at: 2183 2183 Size: 6250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4441 4475 1903 Size: 34 2504 34 3678 Fragments arranged by size: 3678 2504 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3245 4142 5352 3245 Size: 897 1210 4143 Fragments arranged by size: 4143 1210 897 BclI T'GATC_A Cuts at: 1548 1548 Size: 6250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 2213 2286 Size: 107 122 71 631 605 450 91 73 Cuts at: 2286 3535 4861 136 Size: 1249 1326 1525 Fragments arranged by size: 1525 1326 1249 631 605 450 122 107 91 73 71 BglII A'GATC_T Cuts at: 6245 6245 Size: 6250 BlpI GC'TnA_GC Cuts at: 3515 3667 3515 Size: 152 6098 Bme1580I G_kGCm'C Cuts at: 1790 3102 3393 4311 5467 1790 Size: 1312 291 918 1156 2573 Fragments arranged by size: 2573 1312 1156 918 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 3396 3981 Size: 416 53 83 399 24 957 1022 585 Cuts at: 3981 4939 442 Size: 958 1753 Fragments arranged by size: 1753 1022 958 957 585 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2117 2260 3235 4910 1239 Size: 857 21 143 975 1675 2579 Fragments arranged by size: 2579 1675 975 857 143 21 Bpu10I CC'TnA_GC Cuts at: 2183 2183 Size: 6250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3527 4375 5240 5538 3527 Size: 848 865 298 4239 Fragments arranged by size: 4239 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 4913 882 Size: 617 1098 2316 2219 Fragments arranged by size: 2316 2219 1098 617 BsaAI yAC'GTr Cuts at: 493 1204 2212 4041 493 Size: 711 1008 1829 2702 Fragments arranged by size: 2702 1829 1008 711 BsaBI GATnn'nnATC Cuts at: 2366 6244 2366 Size: 3878 2372 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2579 2853 4057 4439 Size: 53 83 186 936 1046 274 1204 382 Cuts at: 4439 5074 275 Size: 635 1451 Fragments arranged by size: 1451 1204 1046 936 635 382 274 186 83 53 BsaWI w'CCGG_w Cuts at: 4683 5081 5355 5502 5913 4683 Size: 398 274 147 411 5020 Fragments arranged by size: 5020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 2873 2903 3029 Size: 30 165 30 1377 30 63 30 126 Cuts at: 3029 3059 1178 Size: 30 4369 Fragments arranged by size: 4369 1377 165 126 63 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2801 3050 1186 Size: 1615 249 4386 Fragments arranged by size: 4386 1615 249 BseYI C'CCAG_C Cuts at: 2640 3010 3403 5453 5949 2640 Size: 370 393 2050 496 2941 Fragments arranged by size: 2941 2050 496 393 370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2963 1163 Size: 573 1227 4450 Fragments arranged by size: 4450 1227 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3474 3700 4461 5489 1540 Size: 275 186 1473 226 761 1028 2301 Fragments arranged by size: 2301 1473 1028 761 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2393 2718 3102 3521 4311 4396 5467 2393 Size: 325 384 419 790 85 1071 3176 Fragments arranged by size: 3176 1071 790 419 384 325 85 BsmI GAATG_Cn' Cuts at: 3174 3761 3854 3174 Size: 587 93 5570 Fragments arranged by size: 5570 587 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3086 3086 Size: 6250 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2393 2718 3102 3393 3441 3521 Size: 399 520 83 325 384 291 48 80 Cuts at: 3521 3569 4311 4396 5467 1391 Size: 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 399 384 325 291 85 83 80 48 48 BspEI T'CCGG_A Cuts at: 5913 5913 Size: 6250 BspHI T'CATG_A Cuts at: 2685 4139 5869 2685 Size: 1454 1730 3066 Fragments arranged by size: 3066 1730 1454 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2409 2460 2985 3605 844 Size: 1565 51 525 620 3489 Fragments arranged by size: 3489 1565 620 525 51 BsrBI CCG'CTC Cuts at: 1394 1823 2526 3448 4137 1394 Size: 429 703 922 689 3507 Fragments arranged by size: 3507 922 703 689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3246 4745 4919 65 Size: 1183 1998 1499 174 1396 Fragments arranged by size: 1998 1499 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2504 2592 4894 5081 1700 Size: 110 694 88 2302 187 2869 Fragments arranged by size: 2869 2302 694 187 110 88 BsrGI T'GTAC_A Cuts at: 96 3045 96 Size: 2949 3301 BssHII G'CGCG_C Cuts at: 1925 3455 1925 Size: 1530 4720 BssSI C'ACGA_G Cuts at: 2044 2294 4310 5322 2044 Size: 250 2016 1012 2972 Fragments arranged by size: 2972 2016 1012 250 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6250 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 2408 1184 Size: 1224 5026 BstXI CCAn_nnnn'nTGG Cuts at: 2869 3260 3421 2869 Size: 391 161 5698 Fragments arranged by size: 5698 391 161 BstYI r'GATC_y Cuts at: 1972 2551 3159 3580 3947 4332 4349 5801 5887 Size: 579 608 421 367 385 17 1452 86 Cuts at: 5887 5899 6245 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 608 579 421 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2119 2119 Size: 6250 BtgI C'CryG_G Cuts at: 513 1065 2004 2608 3432 513 Size: 552 939 604 824 3331 Fragments arranged by size: 3331 939 824 604 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3495 487 Size: 683 56 2269 3242 Fragments arranged by size: 3242 2269 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3766 4560 4580 2111 Size: 1655 794 20 3781 Fragments arranged by size: 3781 1655 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6215 DraI TTT'AAA Cuts at: 3554 3554 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5257 809 Size: 507 3941 1802 Fragments arranged by size: 3941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2287 3316 Size: 54 1425 50 275 18 384 73 1029 Cuts at: 3316 3471 3697 4586 6215 8 Size: 155 226 889 1629 43 Fragments arranged by size: 1629 1425 1029 889 384 275 226 155 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3471 3697 1537 Size: 275 1659 226 4090 Fragments arranged by size: 4090 1659 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 2903 3302 4180 1353 Size: 1550 399 878 3423 Fragments arranged by size: 3423 1550 878 399 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2203 2615 2948 4837 5211 5357 Size: 1064 138 614 412 333 1889 374 146 Cuts at: 5357 387 Size: 1280 Fragments arranged by size: 1889 1280 1064 614 412 374 333 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2117 2131 2260 2927 3235 4313 Size: 393 464 21 14 129 667 308 1078 Cuts at: 4313 4910 5697 5965 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1078 787 667 597 464 393 308 268 129 21 14 EcoO109I rG'GnC_Cy Cuts at: 2220 3040 3353 3390 2220 Size: 820 313 37 5080 Fragments arranged by size: 5080 820 313 37 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 2428 5397 1678 Size: 750 2969 2531 Fragments arranged by size: 2969 2531 750 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 2785 2817 4891 Size: 32 135 32 992 32 189 32 2074 Cuts at: 4891 4923 4965 4997 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2074 992 189 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3594 3840 669 Size: 988 281 1656 246 3079 Fragments arranged by size: 3079 1656 988 281 246 HindIII A'AGCT_T Cuts at: 748 2081 748 Size: 1333 4917 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2859 2988 5364 5548 1305 Size: 1554 129 2376 184 2007 Fragments arranged by size: 2376 2007 1554 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 10 Size: 54 1425 343 384 4044 Fragments arranged by size: 4044 1425 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2867 4208 4567 4726 518 Size: 648 1701 1341 359 159 2042 Fragments arranged by size: 2042 1701 1341 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2202 2492 2610 3403 3666 4343 5491 Size: 468 128 290 118 793 263 677 1148 Cuts at: 5491 5736 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 793 677 468 290 263 245 128 118 NaeI GCC'GGC Cuts at: 2506 2506 Size: 6250 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4759 Fragments arranged by size: 4759 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6250 NgoMIV G'CCGG_C Cuts at: 2504 2504 Size: 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 2962 3608 5153 1132 Size: 97 1733 646 1545 2229 Fragments arranged by size: 2229 1733 1545 646 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 5149 5149 Size: 6250 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5348 PfoI T'CCnGG_A Cuts at: 2568 2568 Size: 6250 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PmlI CAC'GTG Cuts at: 2212 2212 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2807 2839 4319 4351 5832 5864 2807 Size: 32 1480 32 1481 32 3193 Fragments arranged by size: 3193 1481 1480 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 3353 2220 Size: 1133 5117 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PspOMI G'GGCC_C Cuts at: 3389 3389 Size: 6250 PstI C_TGCA'G Cuts at: 830 3602 830 Size: 2772 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 PvuII CAG'CTG Cuts at: 2492 2492 Size: 6250 SacII CC_GC'GG Cuts at: 2611 2611 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SanDI GG'GwC_CC Cuts at: 3353 3353 Size: 6250 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 2588 4498 6210 2588 Size: 1910 1712 2628 Fragments arranged by size: 2628 1910 1712 SexAI A'CCwGG_T Cuts at: 2742 2742 Size: 6250 SfcI C'TryA_G Cuts at: 826 1045 2224 3598 4733 5414 5605 826 Size: 219 1179 1374 1135 681 191 1471 Fragments arranged by size: 1471 1374 1179 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3542 4354 5255 5517 820 Size: 197 926 1599 812 901 262 1553 Fragments arranged by size: 1599 1553 926 901 812 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 4174 5 Size: 47 4122 2081 Fragments arranged by size: 4122 2081 47 StuI AGG'CCT Cuts at: 1517 2094 1517 Size: 577 5673 StyI C'CwwG_G Cuts at: 513 1065 2004 3689 513 Size: 552 939 1685 3074 Fragments arranged by size: 3074 1685 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 4277 4294 4447 4632 5077 325 Size: 1255 1956 741 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2586 3045 4496 Size: 274 80 33 51 1443 609 459 1451 Cuts at: 4496 6208 96 Size: 1712 138 Fragments arranged by size: 1712 1451 1443 609 459 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 6210 6242 2873 Size: 32 3305 32 2881 Fragments arranged by size: 3305 2881 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3586 3586 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 2822 4379 2822 Size: 1557 4693 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2580 2854 4058 276 Size: 53 83 186 936 1046 274 1204 2468 Fragments arranged by size: 2468 1204 1046 936 274 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI