Restriction map for
pFN21AE5661
(Linear) (Six-base) MAPSORT of: KIEE5661.seq Check: 6179 from: 1 to: 1545
KIEE5661.orf
With 182 enzymes: SgfI *
December 5, 2014 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 455 1545
Size: 455 1090
AatII G_ACGT'C
Cuts at: 0 577 851 1545
Size: 577 274 694
Fragments arranged by size:
694 577 274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 922 1545
Size: 126 796 623
Fragments arranged by size:
796 623 126
AhdI GACnn_n'nnGTC
Cuts at: 0 573 1545
Size: 573 972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 463 1545
Size: 268 195 1082
Fragments arranged by size:
1082 268 195
AlwNI CAG_nnn'CTG
Cuts at: 0 381 423 543 1545
Size: 381 42 120 1002
Fragments arranged by size:
1002 381 120 42
ApaI G_GGCC'C
Cuts at: 0 1388 1545
Size: 1388 157
ApaLI G'TGCA_C
Cuts at: 0 1093 1545
Size: 1093 452
ApoI r'AATT_y
Cuts at: 0 558 1077 1126 1545
Size: 558 519 49 419
Fragments arranged by size:
558 519 419 49
AvaI C'yCGr_G
Cuts at: 0 304 1027 1545
Size: 304 723 518
Fragments arranged by size:
723 518 304
BanI G'GyrC_C
Cuts at: 0 496 1405 1545
Size: 496 909 140
Fragments arranged by size:
909 496 140
BanII G_rGCy'C
Cuts at: 0 305 1388 1436 1545
Size: 305 1083 48 109
Fragments arranged by size:
1083 305 109 48
BbvCI CC'TCA_GC
Cuts at: 0 178 1545
Size: 178 1367
BciVI GTATCCnnnnn_n'
Cuts at: 0 1240 1545
Size: 1240 305
BglI GCCn_nnn'nGGC
Cuts at: 0 117 208 281 1530 1545
Size: 117 91 73 1249 15
Fragments arranged by size:
1249 117 91 73 15
BlpI GC'TnA_GC
Cuts at: 0 1510 1545
Size: 1510 35
Bme1580I G_kGCm'C
Cuts at: 0 1097 1388 1545
Size: 1097 291 157
Fragments arranged by size:
1097 291 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 1391 1545
Size: 369 1022 154
Fragments arranged by size:
1022 369 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 112 255 1230 1545
Size: 91 21 143 975 315
Fragments arranged by size:
975 315 143 91 21
Bpu10I CC'TnA_GC
Cuts at: 0 178 1545
Size: 178 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 1545
Size: 1522 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 1545
Size: 592 953
BsaAI yAC'GTr
Cuts at: 0 207 1545
Size: 207 1338
BsaBI GATnn'nnATC
Cuts at: 0 361 1545
Size: 361 1184
BsaHI Gr'CG_yC
Cuts at: 0 574 848 1545
Size: 574 274 697
Fragments arranged by size:
697 574 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 868 898 1024 1054 1545
Size: 775 30 63 30 126 30 491
Fragments arranged by size:
775 491 126 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 796 1045 1545
Size: 796 249 500
Fragments arranged by size:
796 500 249
BseYI C'CCAG_C
Cuts at: 0 635 1005 1398 1545
Size: 635 370 393 147
Fragments arranged by size:
635 393 370 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1545
Size: 958 587
BsiEI CG_ry'CG
Cuts at: 0 1469 1545
Size: 1469 76
BsiHKAI G_wGCw'C
Cuts at: 0 388 713 1097 1516 1545
Size: 388 325 384 419 29
Fragments arranged by size:
419 388 384 325 29
BsmI GAATG_Cn'
Cuts at: 0 1169 1545
Size: 1169 376
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1081 1545
Size: 1081 464
Bsp1286I G_dGCh'C
Cuts at: 0 305 388 713 1097 1388 1436 1516 1545
Size: 305 83 325 384 291 48 80 29
Fragments arranged by size:
384 325 305 291 83 80 48 29
BspHI T'CATG_A
Cuts at: 0 680 1545
Size: 680 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 404 455 980 1545
Size: 404 51 525 565
Fragments arranged by size:
565 525 404 51
BsrBI CCG'CTC
Cuts at: 0 521 1443 1545
Size: 521 922 102
Fragments arranged by size:
922 521 102
BsrDI GCAATG_nn'
Cuts at: 0 1241 1545
Size: 1241 304
BsrFI r'CCGG_y
Cuts at: 0 499 587 1545
Size: 499 88 958
Fragments arranged by size:
958 499 88
BsrGI T'GTAC_A
Cuts at: 0 1040 1545
Size: 1040 505
BssHII G'CGCG_C
Cuts at: 0 1450 1545
Size: 1450 95
BssSI C'ACGA_G
Cuts at: 0 39 289 1545
Size: 39 250 1256
Fragments arranged by size:
1256 250 39
BstEII G'GTnAC_C
Cuts at: 0 403 1545
Size: 403 1142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 864 1255 1416 1545
Size: 864 391 161 129
Fragments arranged by size:
864 391 161 129
BstYI r'GATC_y
Cuts at: 0 546 1154 1545
Size: 546 608 391
Fragments arranged by size:
608 546 391
Bsu36I CC'TnA_GG
Cuts at: 0 114 1545
Size: 114 1431
BtgI C'CryG_G
Cuts at: 0 603 1427 1545
Size: 603 824 118
Fragments arranged by size:
824 603 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1490 1545
Size: 1490 55
BtsI GCAGTG_nn'
Cuts at: 0 106 1545
Size: 106 1439
EaeI y'GGCC_r
Cuts at: 0 209 282 1311 1466 1545
Size: 209 73 1029 155 79
Fragments arranged by size:
1029 209 155 79 73
EagI C'GGCC_G
Cuts at: 0 1466 1545
Size: 1466 79
EarI CTCTTCn'nnn_
Cuts at: 0 898 1297 1545
Size: 898 399 248
Fragments arranged by size:
898 399 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 198 610 943 1545
Size: 198 412 333 602
Fragments arranged by size:
602 412 333 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 112 126 255 922 1230 1545
Size: 91 21 14 129 667 308 315
Fragments arranged by size:
667 315 308 129 91 21 14
EcoO109I rG'GnC_Cy
Cuts at: 0 215 1035 1348 1385 1545
Size: 215 820 313 37 160
Fragments arranged by size:
820 313 215 160 37
HaeII r_GCGC'y
Cuts at: 0 423 1545
Size: 423 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 780 812 1545
Size: 559 32 189 32 733
Fragments arranged by size:
733 559 189 32 32
HindIII A'AGCT_T
Cuts at: 0 76 1545
Size: 76 1469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 983 1545
Size: 854 129 562
Fragments arranged by size:
854 562 129
MscI TGG'CCA
Cuts at: 0 211 1545
Size: 211 1334
MslI CAynn'nnrTG
Cuts at: 0 862 1545
Size: 862 683
MspA1I CmG'CkG
Cuts at: 0 69 197 487 605 1398 1545
Size: 69 128 290 118 793 147
Fragments arranged by size:
793 290 147 128 118 69
NaeI GCC'GGC
Cuts at: 0 501 1545
Size: 501 1044
NgoMIV G'CCGG_C
Cuts at: 0 499 1545
Size: 499 1046
NspI r_CATG'y
Cuts at: 0 957 1545
Size: 957 588
PfoI T'CCnGG_A
Cuts at: 0 563 1545
Size: 563 982
PmlI CAC'GTG
Cuts at: 0 207 1545
Size: 207 1338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 1545
Size: 802 32 711
Fragments arranged by size:
802 711 32
PpuMI rG'GwC_Cy
Cuts at: 0 215 1348 1545
Size: 215 1133 197
Fragments arranged by size:
1133 215 197
PspOMI G'GGCC_C
Cuts at: 0 1384 1545
Size: 1384 161
PvuII CAG'CTG
Cuts at: 0 487 1545
Size: 487 1058
SacII CC_GC'GG
Cuts at: 0 606 1545
Size: 606 939
SanDI GG'GwC_CC
Cuts at: 0 1348 1545
Size: 1348 197
ScaI AGT'ACT
Cuts at: 0 583 1545
Size: 583 962
SexAI A'CCwGG_T
Cuts at: 0 737 1545
Size: 737 808
SfcI C'TryA_G
Cuts at: 0 219 1545
Size: 219 1326
SmlI C'TyrA_G
Cuts at: 0 1537 1545
Size: 1537 8
StuI AGG'CCT
Cuts at: 0 89 1545
Size: 89 1456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1531 1545
Size: 1531 14
TatI w'GTAC_w
Cuts at: 0 581 1040 1545
Size: 581 459 505
Fragments arranged by size:
581 505 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 1545
Size: 868 32 645
Fragments arranged by size:
868 645 32
XmnI GAAnn'nnTTC
Cuts at: 0 817 1545
Size: 817 728
ZraI GAC'GTC
Cuts at: 0 575 849 1545
Size: 575 274 696
Fragments arranged by size:
696 575 274
Enzymes that do cut:
AarI AatII AcuI AhdI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbvCI BciVI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PfoI PmlI PpiI PpuMI PspOMI PvuII SacII
SanDI ScaI SexAI SfcI SmlI StuI TaqII TatI
TstI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BclI BfrBI BglII BmgBI BmtI BplI
BsaWI BsiWI BspEI BstAPI BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SalI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI