################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII BamHI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BsaBI BsgI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MflI MluI MunI MvnI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccIII 990 TCCGGA T^CCGGA AcsI 223 AAATTC [GA]^AATT[CT] AgeI 1216 ACCGGT A^CCGGT AlwI 3 GGATC GGATC(4/5) ApoI 223 AAATTC [GA]^AATT[CT] AseI 1254 ATTAAT AT^TAAT AsnI 1254 ATTAAT AT^TAAT Asp700I 716 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1120 GAGCTC G[AT]GC[AT]^C AspI 247 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 861 GGCGCC G^G[CT][GA]CC BanII 1120 GAGCTC G[GA]GC[CT]^C BbeI 861 GGCGCC GGCGC^C BlpI 293 GCTTAGC GC^T[ACGT]AGC BmyI 1120 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 293 GCTTAGC GC^T[ACGT]AGC BsaAI 1018 TACGTA [CT]AC^GT[GA] BsaI 252 GGTCTC GGTCTC(1/5) BseAI 990 TCCGGA T^CCGGA BseRI 479 GAGGAG GAGGAG(10/8) BsiEI 887 CGGTCG CG[GA][CT]^CG BsiHKAI 1120 GAGCTC G[AT]GC[AT]^C BsmFI 1134 GGGAC GGGAC(10/14) BsmI 145 GCATTC GAATGC(1/-1) Bsp1286I 1120 GAGCTC G[AGT]GC[ACT]^C BspEI 990 TCCGGA T^CCGGA BspLU11I 151 ACATGT A^CATGT BspMI 1237 GCAGGT ACCTGC(4/8) BstXI 1061 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 293 GCTTAGC GC^T[ACGT]AGC CfoI 862 GCGC GCG^C DraI 844 TTTAAA TTT^AAA DraII 402 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1009 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1200 CCTTGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 402 AGGTCCT [GA]G^G[ACGT]CC[CT] HaeII 861 GGCGCC [GA]GCGC^[CT] HgaI 788 GCGTC GACGC(5/10) HhaI 862 GCGC GCG^C HinP1I 862 GCGC G^CGC HincII 1105 GTTGAC GT[CT]^[GA]AC HindII 1105 GTTGAC GT[CT]^[GA]AC KasI 861 GGCGCC G^GCGCC MroI 990 TCCGGA T^CCGGA NaeI 858 GCCGGC GCC^GGC NarI 861 GGCGCC GG^CGCC NcoI 1067 CCATGG C^CATGG NgoMIV 858 GCCGGC G^CCGGC PflFI 247 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 325 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1216 ACCGGT A^CCGGT PpuMI 402 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 1254 ATTAAT AT^TAAT SacI 1120 GAGCTC GAGCT^C SexAI 1100 ACCAGGT A^CC[AT]GGT SfcI 585 CTGTAG C^T[GA][CT]AG SfoI 861 GGCGCC GGC^GCC SnaBI 1018 TACGTA TAC^GTA SspI 327 AATATT AAT^ATT StuI 58 AGGCCT AGG^CCT Tth111I 247 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 325 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 716 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 787 GGCGTC G[GA]^CG[CT]C 861 GGCGCC AflIII 151 ACATGT A^C[GA][CT]GT 773 ACGTGT AlwNI 647 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 740 CAGTTGCTG AvrII 576 CCTAGG C^CTAGG 783 CCTAGG BalI 602 TGGCCA TGG^CCA 1070 TGGCCA BbsI 1224 GAAGAC GAAGAC(2/6) 661 GTCTTC BclI 1367 TGATCA T^GATCA 1379 TGATCA BlnI 576 CCTAGG C^CTAGG 783 CCTAGG BpuAI 1224 GAAGAC GAAGAC(2/6) 661 GTCTTC BsaHI 787 GGCGTC G[GA]^CG[CT]C 861 GGCGCC BsmAI 253 GTCTC GTCTC(1/5) 1191 GTCTC BsrFI 858 GCCGGC [GA]^CCGG[CT] 1216 ACCGGT BsrI 901 ACTGG ACTGG(1/-1) 1282 ACTGG BstF5I 73 CATCC GGATG(2/0) 945 GGATG Cfr10I 858 GCCGGC [GA]^CCGG[CT] 1216 ACCGGT EaeI 602 TGGCCA [CT]^GGCC[GA] 1070 TGGCCA FbaI 1367 TGATCA T^GATCA 1379 TGATCA FokI 73 CATCC GGATG(9/13) 945 GGATG Hin1I 787 GGCGTC G[GA]^CG[CT]C 861 GGCGCC HphI 244 GGTGA GGTGA(8/7) 665 TCACC MaeII 773 ACGT A^CGT 1019 ACGT MluNI 602 TGGCCA TGG^CCA 1070 TGGCCA MscI 602 TGGCCA TGG^CCA 1070 TGGCCA MwoI 304 GCAATGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 508 GCCAGAGATGC NspI 151 ACATGT [GA]CATG^[CT] 657 GCATGT SfaNI 514 GATGC GCATC(5/9) 1024 GATGC SmlI 287 CTCAAG C^T[CT][GA]AG 519 CTTGAG TaqI 447 TCGA T^CGA 1194 TCGA TthHB8I 447 TCGA T^CGA 1194 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BfaI BpmI BsaJI BsaWI BsiYI BslI BsrDI BssKI BstNI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI