(Circular) (Six-base) MAPSORT of: pFN21AE6475.seq Check: 9717 from: 1 to: 6124 pFN21AE6475 6124 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 2267 4187 5571 5839 1632 Size: 405 230 1920 1384 268 1917 Fragments arranged by size: 1920 1917 1384 405 268 230 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 2156 2778 5023 6000 1203 Size: 953 622 2245 977 1327 Fragments arranged by size: 2245 1327 977 953 622 AgeI A'CCGG_T Cuts at: 3221 4955 3221 Size: 1734 4390 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 2276 3335 3380 1166 Size: 1110 1059 45 3910 Fragments arranged by size: 3910 1110 1059 45 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2294 1184 Size: 1110 5014 AlwNI CAG_nnn'CTG Cuts at: 2657 2750 3867 5439 2657 Size: 93 1117 1572 3342 Fragments arranged by size: 3342 1572 1117 93 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2228 3433 3659 3811 5920 1493 Size: 735 1205 226 152 2109 1697 Fragments arranged by size: 2109 1697 1205 735 226 152 AseI AT'TA_AT Cuts at: 160 1032 3260 4678 160 Size: 872 2228 1418 1606 Fragments arranged by size: 2228 1606 1418 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 AvrII C'CTAG_G Cuts at: 2581 2788 2581 Size: 207 5917 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 3445 4900 618 Size: 325 217 627 1079 579 1455 1842 Fragments arranged by size: 1842 1455 1079 627 579 325 217 BanII G_rGCy'C Cuts at: 1391 3129 3443 1391 Size: 1738 314 4072 Fragments arranged by size: 4072 1738 314 BbeI G_GCGC'C Cuts at: 2870 2870 Size: 6124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2659 3236 928 Size: 971 760 577 3816 Fragments arranged by size: 3816 971 760 577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 3372 3384 1548 Size: 1824 12 4288 Fragments arranged by size: 4288 1824 12 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 2299 3541 2299 Size: 1242 4882 Bme1580I G_kGCm'C Cuts at: 1790 4185 5341 1790 Size: 2395 1156 2573 Fragments arranged by size: 2573 2395 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3855 4813 442 Size: 416 53 83 399 24 2438 958 1753 Fragments arranged by size: 2438 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2374 2970 4784 1239 Size: 833 302 596 1814 2579 Fragments arranged by size: 2579 1814 833 596 302 Bpu10I CC'TnA_GC Cuts at: 3085 3085 Size: 6124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2277 2545 4249 5114 5412 2277 Size: 268 1704 865 298 2989 Fragments arranged by size: 2989 1704 865 298 268 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2263 4787 882 Size: 617 764 2524 2219 Fragments arranged by size: 2524 2219 764 617 BsaAI yAC'GTr Cuts at: 493 1204 3025 3915 493 Size: 711 1821 890 2702 Fragments arranged by size: 2702 1821 890 711 BsaBI GATnn'nnATC Cuts at: 6118 6118 Size: 6124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2793 2867 3931 4313 Size: 53 83 186 936 1260 74 1064 382 Cuts at: 4313 4948 275 Size: 635 1451 Fragments arranged by size: 1451 1260 1064 936 635 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 2080 2995 3221 4557 4955 5229 5376 5787 2080 Size: 915 226 1336 398 274 147 411 2417 Fragments arranged by size: 2417 1336 915 411 398 274 226 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 1178 Size: 30 165 30 743 30 5126 Fragments arranged by size: 5126 743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2499 1186 Size: 1313 4811 BseYI C'CCAG_C Cuts at: 2918 5327 5823 2918 Size: 2409 496 3219 Fragments arranged by size: 3219 2409 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2895 3574 4335 5363 1540 Size: 275 186 894 679 761 1028 2301 Fragments arranged by size: 2301 1028 894 761 679 275 186 BsiHKAI G_wGCw'C Cuts at: 3129 4185 4270 5341 3129 Size: 1056 85 1071 3912 Fragments arranged by size: 3912 1071 1056 85 BsmI GAATG_Cn' Cuts at: 2150 3635 3728 2150 Size: 1485 93 4546 Fragments arranged by size: 4546 1485 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3129 3443 4185 4270 5341 1391 Size: 399 1339 314 742 85 1071 2174 Fragments arranged by size: 2174 1339 1071 742 399 314 85 BspEI T'CCGG_A Cuts at: 2995 5787 2995 Size: 2792 3332 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3233 3479 844 Size: 2389 246 3489 Fragments arranged by size: 3489 2389 246 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2316 3033 3129 3305 3387 4619 Size: 1183 879 189 717 96 176 82 1232 Cuts at: 4619 4793 65 Size: 174 1396 Fragments arranged by size: 1396 1232 1183 879 717 189 176 174 96 82 BsrFI r'CCGG_y Cuts at: 1700 1810 2863 3221 4768 4955 1700 Size: 110 1053 358 1547 187 2869 Fragments arranged by size: 2869 1547 1053 358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstXI CCAn_nnnn'nTGG Cuts at: 3073 3073 Size: 6124 BstYI r'GATC_y Cuts at: 1972 3454 3821 4206 4223 5675 5761 5773 6119 Size: 1482 367 385 17 1452 86 12 346 Cuts at: 6119 1972 Size: 1977 Fragments arranged by size: 1977 1482 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2635 2730 3072 513 Size: 552 939 631 95 342 3565 Fragments arranged by size: 3565 939 631 552 342 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 3375 3640 4434 4454 3375 Size: 265 794 20 5045 Fragments arranged by size: 5045 794 265 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 2851 3428 2851 Size: 577 5547 DraIII CAC_nnn'GTG Cuts at: 3019 3019 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2607 3075 3571 Size: 54 1425 50 275 18 777 468 496 Cuts at: 3571 4460 6089 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 777 496 468 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2353 3014 3170 4054 1353 Size: 1000 661 156 884 3423 Fragments arranged by size: 3423 1000 884 661 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3127 3127 Size: 6124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2072 2267 2374 2970 4187 4784 Size: 393 405 35 195 107 596 1217 597 Cuts at: 4784 5571 5839 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1217 787 597 596 405 393 268 195 107 35 EcoNI CCTnn'n_nnAGG Cuts at: 3209 3209 Size: 6124 EcoO109I rG'GnC_Cy Cuts at: 2408 2408 Size: 6124 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 2870 5271 1678 Size: 1192 2401 2531 Fragments arranged by size: 2531 2401 1192 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2520 2552 3376 Size: 32 135 32 671 32 245 32 824 Cuts at: 3376 3408 4765 4797 4839 4871 1373 Size: 32 1357 32 42 32 2626 Fragments arranged by size: 2626 1357 824 671 245 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3112 3468 3714 669 Size: 988 281 1174 356 246 3079 Fragments arranged by size: 3079 1174 988 356 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KasI G'GCGC_C Cuts at: 2866 2866 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2938 3031 5238 5422 1305 Size: 1633 93 2207 184 2007 Fragments arranged by size: 2207 2007 1633 184 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 2609 3077 10 Size: 54 1425 343 777 468 3057 Fragments arranged by size: 3057 1425 777 468 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2155 2189 2276 2546 2675 3071 3335 Size: 648 989 34 87 270 129 396 264 Cuts at: 3335 3380 4082 4441 4600 518 Size: 45 702 359 159 2042 Fragments arranged by size: 2042 989 702 648 396 359 270 264 159 129 87 45 34 MspA1I CmG'CkG Cuts at: 1606 2321 2657 2918 3540 4217 5365 5610 1606 Size: 715 336 261 622 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 622 336 261 245 NaeI GCC'GGC Cuts at: 2865 2865 Size: 6124 NarI GG'CG_CC Cuts at: 2867 2867 Size: 6124 NcoI C'CATG_G Cuts at: 513 1065 2004 3072 513 Size: 552 939 1068 3565 Fragments arranged by size: 3565 1068 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NgoMIV G'CCGG_C Cuts at: 2863 2863 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2160 2666 3482 5027 1132 Size: 97 931 506 816 1545 2229 Fragments arranged by size: 2229 1545 931 816 506 97 PasI CC'CwG_GG Cuts at: 1348 3136 1348 Size: 1788 4336 PciI A'CATG_T Cuts at: 2156 5023 2156 Size: 2867 3257 PflMI CCAn_nnn'nTGG Cuts at: 1108 2336 1108 Size: 1228 4896 PfoI T'CCnGG_A Cuts at: 2088 2479 2088 Size: 391 5733 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2953 2985 4193 4225 5706 5738 2953 Size: 32 1208 32 1481 32 3339 Fragments arranged by size: 3339 1481 1208 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2408 2408 Size: 6124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PstI C_TGCA'G Cuts at: 830 3476 830 Size: 2646 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 2321 2657 2918 2321 Size: 336 261 5527 Fragments arranged by size: 5527 336 261 SacI G_AGCT'C Cuts at: 3129 3129 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SexAI A'CCwGG_T Cuts at: 3105 3105 Size: 6124 SfcI C'TryA_G Cuts at: 826 1045 2590 3472 4607 5288 5479 826 Size: 219 1545 882 1135 681 191 1471 Fragments arranged by size: 1545 1471 1135 882 681 219 191 SfoI GGC'GCC Cuts at: 2868 2868 Size: 6124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2292 2524 4228 5129 5391 820 Size: 197 926 349 232 1704 901 262 1553 Fragments arranged by size: 1704 1553 926 901 349 262 232 197 SnaBI TAC'GTA Cuts at: 493 3025 3915 493 Size: 2532 890 2702 Fragments arranged by size: 2702 2532 890 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 2334 4048 5 Size: 47 2282 1714 2081 Fragments arranged by size: 2282 2081 1714 47 StuI AGG'CCT Cuts at: 1517 2065 1517 Size: 548 5576 StyI C'CwwG_G Cuts at: 513 1065 2004 2581 2788 3072 3563 513 Size: 552 939 577 207 284 491 3074 Fragments arranged by size: 3074 939 577 552 491 284 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 2813 4151 4168 4321 4506 4951 Size: 1255 657 576 1338 17 153 185 445 Cuts at: 4951 325 Size: 1498 Fragments arranged by size: 1498 1338 1255 657 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2159 2343 2741 Size: 274 80 33 51 1443 182 184 398 Cuts at: 2741 4370 6082 96 Size: 1629 1712 138 Fragments arranged by size: 1712 1629 1443 398 274 184 182 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2146 2178 3045 3077 6084 6116 2146 Size: 32 867 32 3007 32 2154 Fragments arranged by size: 3007 2154 867 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2255 1316 Size: 939 5185 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 2725 4253 2725 Size: 1528 4596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI