Restriction map for
pFN21AE6475
(Linear) (Six-base) MAPSORT of: KIEE6475.seq Check: 4921 from: 1 to: 1419
KIEE6475.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 262 1419
Size: 32 230 1157
Fragments arranged by size:
1157 230 32
AflIII A'CryG_T
Cuts at: 0 151 773 1419
Size: 151 622 646
Fragments arranged by size:
646 622 151
AgeI A'CCGG_T
Cuts at: 0 1216 1419
Size: 1216 203
AleI CACnn'nnGTG
Cuts at: 0 271 1330 1375 1419
Size: 271 1059 45 44
Fragments arranged by size:
1059 271 45 44
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 289 1419
Size: 289 1130
AlwNI CAG_nnn'CTG
Cuts at: 0 652 745 1419
Size: 652 93 674
Fragments arranged by size:
674 652 93
ApoI r'AATT_y
Cuts at: 0 223 1419
Size: 223 1196
AseI AT'TA_AT
Cuts at: 0 1255 1419
Size: 1255 164
AvrII C'CTAG_G
Cuts at: 0 576 783 1419
Size: 576 207 636
Fragments arranged by size:
636 576 207
BanI G'GyrC_C
Cuts at: 0 861 1419
Size: 861 558
BanII G_rGCy'C
Cuts at: 0 1124 1419
Size: 1124 295
BbeI G_GCGC'C
Cuts at: 0 865 1419
Size: 865 554
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 1231 1419
Size: 654 577 188
Fragments arranged by size:
654 577 188
BclI T'GATC_A
Cuts at: 0 1367 1379 1419
Size: 1367 12 40
Fragments arranged by size:
1367 40 12
BlpI GC'TnA_GC
Cuts at: 0 294 1419
Size: 294 1125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 369 965 1419
Size: 67 302 596 454
Fragments arranged by size:
596 454 302 67
Bpu10I CC'TnA_GC
Cuts at: 0 1080 1419
Size: 1080 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 540 1419
Size: 272 268 879
Fragments arranged by size:
879 272 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 258 1419
Size: 258 1161
BsaAI yAC'GTr
Cuts at: 0 1020 1419
Size: 1020 399
BsaHI Gr'CG_yC
Cuts at: 0 788 862 1419
Size: 788 74 557
Fragments arranged by size:
788 557 74
BsaWI w'CCGG_w
Cuts at: 0 75 990 1216 1419
Size: 75 915 226 203
Fragments arranged by size:
915 226 203 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 1419
Size: 141 30 1248
Fragments arranged by size:
1248 141 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 494 1419
Size: 494 925
BseYI C'CCAG_C
Cuts at: 0 913 1419
Size: 913 506
BsiEI CG_ry'CG
Cuts at: 0 890 1419
Size: 890 529
BsiHKAI G_wGCw'C
Cuts at: 0 1124 1419
Size: 1124 295
BsmI GAATG_Cn'
Cuts at: 0 145 1419
Size: 145 1274
Bsp1286I G_dGCh'C
Cuts at: 0 1124 1419
Size: 1124 295
BspEI T'CCGG_A
Cuts at: 0 990 1419
Size: 990 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1228 1419
Size: 1228 191
BsrDI GCAATG_nn'
Cuts at: 0 122 311 1028 1124 1300 1382 1419
Size: 122 189 717 96 176 82 37
Fragments arranged by size:
717 189 176 122 96 82 37
BsrFI r'CCGG_y
Cuts at: 0 858 1216 1419
Size: 858 358 203
Fragments arranged by size:
858 358 203
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1068 1419
Size: 1068 351
BtgI C'CryG_G
Cuts at: 0 630 725 1067 1419
Size: 630 95 342 352
Fragments arranged by size:
630 352 342 95
BtsI GCAGTG_nn'
Cuts at: 0 1370 1419
Size: 1370 49
DraI TTT'AAA
Cuts at: 0 846 1419
Size: 846 573
DraIII CAC_nnn'GTG
Cuts at: 0 1014 1419
Size: 1014 405
EaeI y'GGCC_r
Cuts at: 0 602 1070 1419
Size: 602 468 349
Fragments arranged by size:
602 468 349
EarI CTCTTCn'nnn_
Cuts at: 0 348 1009 1165 1419
Size: 348 661 156 254
Fragments arranged by size:
661 348 254 156
EcoICRI GAG'CTC
Cuts at: 0 1122 1419
Size: 1122 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 67 262 369 965 1419
Size: 32 35 195 107 596 454
Fragments arranged by size:
596 454 195 107 35 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1204 1419
Size: 1204 215
EcoO109I rG'GnC_Cy
Cuts at: 0 403 1419
Size: 403 1016
HaeII r_GCGC'y
Cuts at: 0 865 1419
Size: 865 554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 515 547 1371 1403 1419
Size: 238 32 245 32 824 32 16
Fragments arranged by size:
824 245 238 32 32 32 16
HincII GTy'rAC
Cuts at: 0 1107 1419
Size: 1107 312
KasI G'GCGC_C
Cuts at: 0 861 1419
Size: 861 558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1026 1419
Size: 933 93 393
Fragments arranged by size:
933 393 93
MscI TGG'CCA
Cuts at: 0 604 1072 1419
Size: 604 468 347
Fragments arranged by size:
604 468 347
MslI CAynn'nnrTG
Cuts at: 0 150 184 271 541 670 1066 1330 1375
Size: 150 34 87 270 129 396 264 45
Cuts at: 1375 1419
Size: 44
Fragments arranged by size:
396 270 264 150 129 87 45 44
34
MspA1I CmG'CkG
Cuts at: 0 316 652 913 1419
Size: 316 336 261 506
Fragments arranged by size:
506 336 316 261
NaeI GCC'GGC
Cuts at: 0 860 1419
Size: 860 559
NarI GG'CG_CC
Cuts at: 0 862 1419
Size: 862 557
NcoI C'CATG_G
Cuts at: 0 1067 1419
Size: 1067 352
NgoMIV G'CCGG_C
Cuts at: 0 858 1419
Size: 858 561
NspI r_CATG'y
Cuts at: 0 155 661 1419
Size: 155 506 758
Fragments arranged by size:
758 506 155
PasI CC'CwG_GG
Cuts at: 0 1131 1419
Size: 1131 288
PciI A'CATG_T
Cuts at: 0 151 1419
Size: 151 1268
PflMI CCAn_nnn'nTGG
Cuts at: 0 331 1419
Size: 331 1088
PfoI T'CCnGG_A
Cuts at: 0 83 474 1419
Size: 83 391 945
Fragments arranged by size:
945 391 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 948 980 1419
Size: 948 32 439
Fragments arranged by size:
948 439 32
PpuMI rG'GwC_Cy
Cuts at: 0 403 1419
Size: 403 1016
PvuII CAG'CTG
Cuts at: 0 316 652 913 1419
Size: 316 336 261 506
Fragments arranged by size:
506 336 316 261
SacI G_AGCT'C
Cuts at: 0 1124 1419
Size: 1124 295
SexAI A'CCwGG_T
Cuts at: 0 1100 1419
Size: 1100 319
SfcI C'TryA_G
Cuts at: 0 585 1419
Size: 585 834
SfoI GGC'GCC
Cuts at: 0 863 1419
Size: 863 556
SmlI C'TyrA_G
Cuts at: 0 287 519 1419
Size: 287 232 900
Fragments arranged by size:
900 287 232
SnaBI TAC'GTA
Cuts at: 0 1020 1419
Size: 1020 399
SspI AAT'ATT
Cuts at: 0 329 1419
Size: 329 1090
StuI AGG'CCT
Cuts at: 0 60 1419
Size: 60 1359
StyI C'CwwG_G
Cuts at: 0 576 783 1067 1419
Size: 576 207 284 352
Fragments arranged by size:
576 352 284 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 808 1419
Size: 232 576 611
Fragments arranged by size:
611 576 232
TatI w'GTAC_w
Cuts at: 0 154 338 736 1419
Size: 154 184 398 683
Fragments arranged by size:
683 398 184 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 141 173 1040 1072 1419
Size: 141 32 867 32 347
Fragments arranged by size:
867 347 141 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 250 1419
Size: 250 1169
XmnI GAAnn'nnTTC
Cuts at: 0 720 1419
Size: 720 699
Enzymes that do cut:
AcuI AflIII AgeI AleI AlfI AlwNI ApoI AseI
AvrII BanI BanII BbeI BbsI BclI BlpI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI
BsrFI BstXI BtgI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PpiI PpuMI PvuII
SacI SexAI SfcI SfoI SmlI SnaBI SspI StuI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AloI ApaI ApaLI AscI AsiSI AvaI BaeI BamHI
BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmrI BmtI BplI BsaBI BsgI BsiWI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SpeI SphI SrfI
SwaI XbaI XcmI XhoI XmaI ZraI