Restriction map for
pFN21AE6652
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# NO CUT ENZYMES ( 169 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 664 GACGTC GACGT^C
AccI 412 GTCTAC GT^[AC][GT]AC
AlwNI 589 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 510 GTGCTC G[AT]GC[AT]^C
BclI 467 TGATCA T^GATCA
BmyI 510 GTGCTC G[AGT]GC[ACT]^C
BseRI 700 GAGGAG GAGGAG(10/8)
BsiHKAI 510 GTGCTC G[AT]GC[AT]^C
BsmI 189 GAATGC GAATGC(1/-1)
Bsp1286I 510 GTGCTC G[AGT]GC[ACT]^C
BsrDI 632 CATTGC GCAATG(2/0)
BsrI 425 CCAGT ACTGG(1/-1)
BstAPI 627 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 264 CCATGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 432 GGATCT [GA]^GATC[CT]
Cac8I 96 GCTGGC GC[ACGT]^[ACGT]GC
DraI 105 TTTAAA TTT^AAA
DraII 590 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 513 CTCTTC CTCTTC(1/4)
EcoO109I 590 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 467 TGATCA T^GATCA
HgaI 319 GACGC GACGC(5/10)
HinfI 597 GACTC G^A[ACGT]TC
Ksp632I 513 CTCTTC CTCTTC(1/4)
MflI 432 GGATCT [GA]^GATC[CT]
MseI 106 TTAA T^TAA
MslI 201 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 547 CCATGG C^CATGG
NspI 243 GCATGT [GA]CATG^[CT]
PleI 597 GACTC GAGTC(4/5)
PstI 346 CTGCAG CTGCA^G
SapI 512 GCTCTTC GCTCTTC(1/4)
SfaNI 683 GCATC GCATC(5/9)
SfcI 346 CTGCAG C^T[GA][CT]AG
SmlI 599 CTCAAG C^T[CT][GA]AG
Tru9I 106 TTAA T^TAA
XhoII 432 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 387 CCGC CCGC(-3/-1)
656 CCGC
AcyI 319 GACGCC G[GA]^CG[CT]C
664 GACGTC
AfaI 291 GTAC GT^AC
384 GTAC
AluI 95 AGCT AG^CT
161 AGCT
AlwI 221 GATCC GGATC(4/5)
432 GGATC
BbrPI 541 CACGTG CAC^GTG
622 CACGTG
BpmI 85 CTCCAG CTGGAG(16/14)
272 CTGGAG
BsaAI 541 CACGTG [CT]AC^GT[GA]
622 CACGTG
BsaHI 319 GACGCC G[GA]^CG[CT]C
664 GACGTC
BsgI 32 GTGCAG GTGCAG(16/14)
625 GTGCAG
BsiYI 687 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
688 CCCTTTTTGGG
BslI 687 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
688 CCCTTTTTGGG
BssKI 13 CCAGG ^CC[ACGT]GG
588 CCAGG
BstNI 13 CCAGG CC^[AT]GG
588 CCAGG
DsaI 547 CCATGG C^C[GA][CT]GG
609 CCACGG
EcoRII 13 CCAGG ^CC[AT]GG
588 CCAGG
EcoT14I 182 CCAAGG C^C[AT][AT]GG
547 CCATGG
HaeIII 423 GGCC GG^CC
591 GGCC
Hin1I 319 GACGCC G[GA]^CG[CT]C
664 GACGTC
HphI 207 GGTGA GGTGA(8/7)
312 GGTGA
MspA1I 387 CCGCTG C[AC]G^C[GT]G
656 CCGCTG
MvaI 13 CCAGG CC^[AT]GG
588 CCAGG
MwoI 537 GCCCCACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
627 GCAGACATTGC
PmaCI 541 CACGTG CAC^GTG
622 CACGTG
PmlI 541 CACGTG CAC^GTG
622 CACGTG
RsaI 291 GTAC GT^AC
384 GTAC
ScrFI 13 CCAGG CC^[ACGT]GG
588 CCAGG
StyI 182 CCAAGG C^C[AT][AT]GG
547 CCATGG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AvaII BbvI BsaJI BsmFI BstF5I
Cfr13I DdeI DpnII Fnu4HI FokI
ItaI MaeII MaeIII MboI MboII
MnlI NdeII NlaIII NlaIV Sau3AI
Sau96I TseI Tsp45I TspRI