(Circular) (Six-base) MAPSORT of: pFN21AE6652.seq Check: 8663 from: 1 to: 5440 pFN21AE6652 5440 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2673 3250 278 Size: 53 83 186 936 1137 577 2468 Fragments arranged by size: 2468 1137 936 577 186 83 53 AccI GT'mk_AC Cuts at: 1937 2418 2783 1937 Size: 481 365 4594 Fragments arranged by size: 4594 481 365 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 2505 3503 4887 5155 1632 Size: 830 43 998 1384 268 1917 Fragments arranged by size: 1917 1384 998 830 268 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 2599 3183 4755 2599 Size: 584 1572 3284 Fragments arranged by size: 3284 1572 584 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2765 1870 Size: 73 822 4545 Fragments arranged by size: 4545 822 73 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 4216 618 Size: 325 217 627 974 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 217 BanII G_rGCy'C Cuts at: 1391 2759 1391 Size: 1368 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 2472 1548 Size: 924 4516 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4051 136 Size: 107 122 71 631 605 2379 1525 Fragments arranged by size: 2379 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 3501 4657 1790 Size: 1711 1156 2573 Fragments arranged by size: 2573 1711 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2298 4100 1239 Size: 836 223 1802 2579 Fragments arranged by size: 2579 1802 836 223 Bpu10I CC'TnA_GC Cuts at: 2642 2642 Size: 5440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2589 3565 4430 4728 2589 Size: 976 865 298 3301 Fragments arranged by size: 3301 976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2548 2629 3231 493 Size: 711 1344 81 602 2702 Fragments arranged by size: 2702 1344 711 602 81 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 2670 3247 3629 Size: 53 83 186 936 792 345 577 382 Cuts at: 3629 4264 275 Size: 635 1451 Fragments arranged by size: 1451 936 792 635 577 382 345 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 1178 Size: 30 165 30 798 30 4387 Fragments arranged by size: 4387 798 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 1186 Size: 1534 3906 BseYI C'CCAG_C Cuts at: 2130 4643 5139 2130 Size: 2513 496 2431 Fragments arranged by size: 2513 2431 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 2651 1163 Size: 573 322 593 3952 Fragments arranged by size: 3952 593 573 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2890 3651 4679 1540 Size: 275 186 889 761 1028 2301 Fragments arranged by size: 2301 1028 889 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2519 3501 3586 4657 2519 Size: 982 85 1071 3302 Fragments arranged by size: 3302 1071 982 85 BsmI GAATG_Cn' Cuts at: 2200 2951 3044 2200 Size: 751 93 4596 Fragments arranged by size: 4596 751 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2519 2759 3501 3586 4657 1391 Size: 399 729 240 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 399 240 85 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2795 844 Size: 1951 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3327 1394 Size: 429 1504 3507 Fragments arranged by size: 3507 1504 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2636 3935 4109 65 Size: 1183 1388 1299 174 1396 Fragments arranged by size: 1396 1388 1299 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 2638 794 Size: 1844 3596 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2276 2276 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2437 2770 3137 3522 3539 4991 5077 5089 Size: 465 333 367 385 17 1452 86 12 Cuts at: 5089 5435 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 465 385 367 346 333 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2552 2614 513 Size: 552 939 548 62 3339 Fragments arranged by size: 3339 939 552 548 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3750 3770 2956 Size: 794 20 4626 Fragments arranged by size: 4626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2112 2744 2112 Size: 632 4808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2887 3776 5405 Size: 54 1425 50 275 18 1057 889 1629 Cuts at: 5405 8 Size: 43 Fragments arranged by size: 1629 1425 1057 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2524 3370 1353 Size: 1171 846 3423 Fragments arranged by size: 3423 1171 846 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4027 4401 4547 387 Size: 1064 138 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2298 2462 2505 3503 4100 4887 Size: 393 443 223 164 43 998 597 787 Cuts at: 4887 5155 1239 Size: 268 1524 Fragments arranged by size: 1524 998 787 597 443 393 268 223 164 43 EcoO109I rG'GnC_Cy Cuts at: 2596 2596 Size: 5440 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 4587 1678 Size: 2909 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373 Size: 32 135 32 2509 32 42 32 2626 Fragments arranged by size: 2626 2509 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3618 Fragments arranged by size: 3618 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 3398 3757 3916 518 Size: 648 1044 1188 359 159 2042 Fragments arranged by size: 2042 1188 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2394 2663 2856 3533 4681 4926 1606 Size: 788 269 193 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 677 269 245 193 NcoI C'CATG_G Cuts at: 513 1065 2004 2552 513 Size: 552 939 548 3401 Fragments arranged by size: 3401 939 552 548 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2252 2798 4343 1132 Size: 97 1023 546 1545 2229 Fragments arranged by size: 2229 1545 1023 546 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PmlI CAC'GTG Cuts at: 2548 2629 2548 Size: 81 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PstI C_TGCA'G Cuts at: 830 2355 2792 830 Size: 1525 437 3478 Fragments arranged by size: 3478 1525 437 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SapI GCTCTTCn'nnn_ Cuts at: 2524 2524 Size: 5440 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2351 2788 3923 4604 4795 826 Size: 219 1306 437 1135 681 191 1471 Fragments arranged by size: 1471 1306 1135 681 437 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2604 3544 4445 4707 820 Size: 197 926 661 940 901 262 1553 Fragments arranged by size: 1553 940 926 901 661 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 2552 2879 513 Size: 552 939 183 365 327 3074 Fragments arranged by size: 3074 939 552 365 327 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2238 2517 3467 3484 3637 3822 4267 Size: 1255 658 279 950 17 153 185 445 Cuts at: 4267 325 Size: 1498 Fragments arranged by size: 1498 1255 950 658 445 279 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 3686 5398 Size: 274 80 33 51 1443 318 1391 1712 Cuts at: 5398 96 Size: 138 Fragments arranged by size: 1712 1443 1391 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 5400 5432 2201 Size: 32 3167 32 2209 Fragments arranged by size: 3167 2209 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2671 3248 276 Size: 53 83 186 936 1137 577 2468 Fragments arranged by size: 2468 1137 936 577 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI