Restriction map for
pFN21AE5567
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII BalI BbeI BbrPI
BciVI BclI BcnI BfrI BglII
BlpI Bpu1102I BsaAI BsaHI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KspI MaeII
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1027 GGTACC G^GTACC
AccI 1227 GTCTAC GT^[AC][GT]AC
AccIII 878 TCCGGA T^CCGGA
AcsI 678 AAATTC [GA]^AATT[CT]
AfaI 1028 GTAC GT^AC
AocI 505 CCTGAGG CC^T[ACGT]AGG
Aor51HI 155 AGCGCT AGC^GCT
ApoI 678 AAATTC [GA]^AATT[CT]
Asp718I 1027 GGTACC G^GTACC
AspHI 693 GAGCAC G[AT]GC[AT]^C
AvaI 1126 CTCGGG C^[CT]CG[GA]G
AvrII 310 CCTAGG C^CTAGG
BbsI 1192 GAAGAC GAAGAC(2/6)
BbvCI 237 GCTGAGG CCTCAGC(-5/-2)
BcgI 406 GCAGGACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 311 CTAG C^TAG
BglI 571 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 310 CCTAGG C^CTAGG
BpuAI 1192 GAAGAC GAAGAC(2/6)
BsaBI 92 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 878 TCCGGA T^CCGGA
BsgI 331 GTGCAG GTGCAG(16/14)
BsiHKAI 693 GAGCAC G[AT]GC[AT]^C
BsmBI 253 CGTCTC CGTCTC(1/5)
BsoBI 1126 CTCGGG C^[CT]CG[GA]G
BspEI 878 TCCGGA T^CCGGA
BspMI 1030 ACCTGC ACCTGC(4/8)
BstXI 652 CCAGAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 505 CCTGAGG CC^T[ACGT]AGG
CfoI 156 GCGC GCG^C
Eco47III 155 AGCGCT AGC^GCT
Eco81I 505 CCTGAGG CC^T[ACGT]AGG
FauI 149 CCCGC CCCGC(4/6)
HaeII 155 AGCGCT [GA]GCGC^[CT]
HgaI 1138 GACGC GACGC(5/10)
HhaI 156 GCGC GCG^C
HinP1I 156 GCGC G^CGC
KpnI 1027 GGTACC GGTAC^C
MaeI 311 CTAG C^TAG
MamI 92 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 878 TCCGGA T^CCGGA
MseI 451 TTAA T^TAA
NspI 541 ACATGC [GA]CATG^[CT]
PflMI 101 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 1028 GTAC GT^AC
SexAI 1023 ACCAGGT A^CC[AT]GGT
Tru9I 451 TTAA T^TAA
Van91I 101 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
ApaI 1237 GGGCCC GGGCC^C
1253 GGGCCC
BamHI 495 GGATCC G^GATCC
1173 GGATCC
BsaWI 878 TCCGGA [AT]^CCGG[AT]
998 ACCGGA
BsrDI 915 CATTGC GCAATG(2/0)
960 GCAATG
BssSI 196 CACGAG CACGAG(-5/-1)
280 CTCGTG
DsaI 120 CCGTGG C^C[GA][CT]GG
1109 CCGTGG
EarI 76 GAAGAG CTCTTC(1/4)
436 GAAGAG
EcoT14I 310 CCTAGG C^C[AT][AT]GG
371 CCTTGG
Ksp632I 76 GAAGAG CTCTTC(1/4)
436 GAAGAG
MslI 562 CACCTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
792 CATGAAGGTG
MspA1I 185 CAGCTG C[AC]G^C[GT]G
481 CAGCTG
PvuII 185 CAGCTG CAG^CTG
481 CAGCTG
SfaNI 181 GATGC GCATC(5/9)
31 GCATC
StuI 143 AGGCCT AGG^CCT
200 AGGCCT
StyI 310 CCTAGG C^C[AT][AT]GG
371 CCTTGG
Tsp45I 887 GTCAC ^GT[GC]AC
1136 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfcI
TfiI TseI Tsp509I TspRI XcmI
XhoII