(Circular) (Six-base) MAPSORT of: pFN21AE5567.seq Check: 3384 from: 1 to: 6004 pFN21AE5567 6004 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3233 3347 1937 Size: 1296 114 4594 Fragments arranged by size: 4594 1296 114 Acc65I G'GTAC_C Cuts at: 3032 3325 3032 Size: 293 5711 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2076 2149 2194 3204 4067 5451 5719 1632 Size: 444 73 45 1010 863 1384 268 1917 Fragments arranged by size: 1917 1384 1010 863 444 268 73 45 AfeI AGC'GCT Cuts at: 2162 2162 Size: 6004 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2054 2275 2302 3747 5319 2054 Size: 221 27 1445 1572 2739 Fragments arranged by size: 2739 1572 1445 221 27 ApaI G_GGCC'C Cuts at: 3246 3262 3246 Size: 16 5988 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2683 3313 3539 3691 5800 1493 Size: 1190 630 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 630 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3131 3329 1870 Size: 73 1188 198 4545 Fragments arranged by size: 4545 1188 198 73 AvrII C'CTAG_G Cuts at: 2315 2315 Size: 6004 BamHI G'GATC_C Cuts at: 2500 3178 3334 3701 2500 Size: 678 156 367 4803 Fragments arranged by size: 4803 678 367 156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 2584 3032 3325 4780 Size: 325 217 627 604 193 448 293 1455 Cuts at: 4780 618 Size: 1842 Fragments arranged by size: 1842 1455 627 604 448 325 293 217 193 BanII G_rGCy'C Cuts at: 1391 2227 3076 3246 3262 3323 1391 Size: 836 849 170 16 61 4072 Fragments arranged by size: 4072 849 836 170 61 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3204 928 Size: 971 1305 3728 Fragments arranged by size: 3728 1305 971 BbvCI CC'TCA_GC Cuts at: 2243 2243 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2400 2434 4195 4229 1903 Size: 34 463 34 1761 34 3678 Fragments arranged by size: 3678 1761 463 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2582 4615 136 Size: 107 122 71 631 605 910 2033 1525 Fragments arranged by size: 2033 1525 910 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 3246 3262 4065 5221 1790 Size: 1456 16 803 1156 2573 Fragments arranged by size: 2573 1456 1156 803 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2827 3735 4693 Size: 416 53 83 399 24 1410 908 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1410 958 908 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2880 2880 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2312 2767 2981 3077 4664 1239 Size: 1058 15 455 214 96 1587 2579 Fragments arranged by size: 2579 1587 1058 455 214 96 15 Bpu10I CC'TnA_GC Cuts at: 2243 2733 2933 2243 Size: 490 200 5314 Fragments arranged by size: 5314 490 200 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2393 2438 2825 4667 882 Size: 617 894 45 387 1842 2219 Fragments arranged by size: 2219 1842 894 617 387 45 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 2101 5998 2101 Size: 3897 2107 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2883 3003 4437 4835 5109 5256 5667 2883 Size: 120 1434 398 274 147 411 3220 Fragments arranged by size: 3220 1434 411 398 274 147 120 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2601 2631 3099 3129 1178 Size: 30 165 30 1198 30 468 30 4053 Fragments arranged by size: 4053 1198 468 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2843 3065 1186 Size: 1192 465 222 4125 Fragments arranged by size: 4125 1192 465 222 BseYI C'CCAG_C Cuts at: 3174 3262 5207 5703 3174 Size: 88 1945 496 3475 Fragments arranged by size: 3475 1945 496 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 1163 Size: 573 621 4810 Fragments arranged by size: 4810 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2702 4065 4150 5221 2702 Size: 1363 85 1071 3485 Fragments arranged by size: 3485 1363 1071 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 BsmBI CGTCTCn'nnnn_ Cuts at: 2264 2264 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2227 2702 3076 3246 3262 3323 4065 Size: 399 437 475 374 170 16 61 742 Cuts at: 4065 4150 5221 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 437 399 374 170 85 61 16 BspEI T'CCGG_A Cuts at: 2883 5667 2883 Size: 2784 3220 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 3359 844 Size: 2200 315 3489 Fragments arranged by size: 3489 2200 315 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2919 2972 4499 4673 65 Size: 1183 1671 53 1527 174 1396 Fragments arranged by size: 1671 1527 1396 1183 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 2201 2285 4064 5076 2201 Size: 84 1779 1012 3129 Fragments arranged by size: 3129 1779 1012 84 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6004 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2664 2011 Size: 653 5351 BstYI r'GATC_y Cuts at: 1972 2253 2271 2500 3178 3334 3701 4086 4103 Size: 281 18 229 678 156 367 385 17 Cuts at: 4103 5555 5641 5653 5999 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 678 385 367 346 281 229 156 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2511 2511 Size: 6004 BtgI C'CryG_G Cuts at: 513 1065 2004 2125 3114 513 Size: 552 939 121 989 3403 Fragments arranged by size: 3403 989 939 552 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2700 3520 4314 4334 2700 Size: 820 794 20 4370 Fragments arranged by size: 4370 820 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2577 2612 2762 2797 529 Size: 35 2013 35 150 35 3736 Fragments arranged by size: 3736 2013 150 35 35 35 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969 Size: 54 1425 50 275 18 1621 889 1629 Cuts at: 5969 8 Size: 43 Fragments arranged by size: 1629 1621 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2436 3934 1353 Size: 723 360 1498 3423 Fragments arranged by size: 3423 1498 723 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2149 2194 2297 2312 2767 2981 Size: 393 444 73 45 103 15 455 214 Cuts at: 2981 3077 3204 4067 4664 5451 5719 1239 Size: 96 127 863 597 787 268 1524 Fragments arranged by size: 1524 863 787 597 455 444 393 268 214 127 103 96 73 45 15 EcoO109I rG'GnC_Cy Cuts at: 2451 3061 3242 3258 3259 2451 Size: 610 181 16 1 5196 Fragments arranged by size: 5196 610 181 16 1 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2164 5151 1678 Size: 486 2987 2531 Fragments arranged by size: 2987 2531 486 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2504 2536 4645 Size: 32 135 32 542 32 358 32 2109 Cuts at: 4645 4677 4719 4751 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2109 542 358 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3036 3329 3036 Size: 293 5711 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5118 5302 1305 Size: 3813 184 2007 Fragments arranged by size: 3813 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2571 2801 3962 4321 4480 518 Size: 648 843 562 230 1161 359 159 2042 Fragments arranged by size: 2042 1161 843 648 562 359 230 159 MspA1I CmG'CkG Cuts at: 1606 2192 2488 3420 4097 5245 5490 1606 Size: 586 296 932 677 1148 245 2120 Fragments arranged by size: 2120 1148 932 677 586 296 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2550 3362 4907 1132 Size: 97 1321 812 1545 2229 Fragments arranged by size: 2229 1545 1321 812 97 PasI CC'CwG_GG Cuts at: 1348 3238 1348 Size: 1890 4114 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 2112 1108 Size: 1004 5000 PfoI T'CCnGG_A Cuts at: 2274 2274 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 2681 3574 2681 Size: 893 5111 PspOMI G'GGCC_C Cuts at: 3242 3258 3242 Size: 16 5988 PstI C_TGCA'G Cuts at: 830 2413 2493 2707 3356 830 Size: 1583 80 214 649 3478 Fragments arranged by size: 3478 1583 649 214 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2192 2488 2192 Size: 296 5708 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SexAI A'CCwGG_T Cuts at: 3028 3028 Size: 6004 SfcI C'TryA_G Cuts at: 826 1045 2409 2489 2703 2974 3352 4487 5168 Size: 219 1364 80 214 271 378 1135 681 Cuts at: 5168 5359 826 Size: 191 1471 Fragments arranged by size: 1471 1364 1135 681 378 271 219 214 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 2150 2207 1517 Size: 633 57 5314 Fragments arranged by size: 5314 633 57 StyI C'CwwG_G Cuts at: 513 1065 2004 2315 2376 3443 513 Size: 552 939 311 61 1067 3074 Fragments arranged by size: 3074 1067 939 552 311 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2323 3039 4031 4048 4201 4386 4831 Size: 1255 743 716 992 17 153 185 445 Cuts at: 4831 325 Size: 1498 Fragments arranged by size: 1498 1255 992 743 716 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4250 5962 96 Size: 274 80 33 51 1443 2273 1712 138 Fragments arranged by size: 2273 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3099 3131 5964 5996 3099 Size: 32 2833 32 3107 Fragments arranged by size: 3107 2833 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2354 2502 2842 3281 2354 Size: 148 340 439 5077 Fragments arranged by size: 5077 439 340 148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 4133 4133 Size: 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI