(Linear) (Six-base) MAPSORT of: KIEE5567.seq Check: 1821 from: 1 to: 1299 KIEE5567.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AccI GT'mk_AC Cuts at: 0 1228 1299 Size: 1228 71 Acc65I G'GTAC_C Cuts at: 0 1027 1299 Size: 1027 272 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 144 189 1199 1299 Size: 71 73 45 1010 100 Fragments arranged by size: 1010 100 73 71 45 AfeI AGC'GCT Cuts at: 0 157 1299 Size: 157 1142 AlwNI CAG_nnn'CTG Cuts at: 0 49 270 297 1299 Size: 49 221 27 1002 Fragments arranged by size: 1002 221 49 27 ApaI G_GGCC'C Cuts at: 0 1241 1257 1299 Size: 1241 16 42 Fragments arranged by size: 1241 42 16 ApoI r'AATT_y Cuts at: 0 678 1299 Size: 678 621 AvaI C'yCGr_G Cuts at: 0 1126 1299 Size: 1126 173 AvrII C'CTAG_G Cuts at: 0 310 1299 Size: 310 989 BamHI G'GATC_C Cuts at: 0 495 1173 1299 Size: 495 678 126 Fragments arranged by size: 678 495 126 BanI G'GyrC_C Cuts at: 0 386 579 1027 1299 Size: 386 193 448 272 Fragments arranged by size: 448 386 272 193 BanII G_rGCy'C Cuts at: 0 222 1071 1241 1257 1299 Size: 222 849 170 16 42 Fragments arranged by size: 849 222 170 42 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 1199 1299 Size: 1199 100 BbvCI CC'TCA_GC Cuts at: 0 238 1299 Size: 238 1061 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 395 429 1299 Size: 395 34 870 Fragments arranged by size: 870 395 34 BglI GCCn_nnn'nGGC Cuts at: 0 577 1299 Size: 577 722 Bme1580I G_kGCm'C Cuts at: 0 1241 1257 1299 Size: 1241 16 42 Fragments arranged by size: 1241 42 16 BmrI ACTGGGnnnn_n' Cuts at: 0 822 1299 Size: 822 477 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 875 1299 Size: 875 424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 307 762 976 1072 1299 Size: 292 15 455 214 96 227 Fragments arranged by size: 455 292 227 214 96 15 Bpu10I CC'TnA_GC Cuts at: 0 238 728 928 1299 Size: 238 490 200 371 Fragments arranged by size: 490 371 238 200 BsaI GGTCTCn'nnnn_ Cuts at: 0 388 433 820 1299 Size: 388 45 387 479 Fragments arranged by size: 479 388 387 45 BsaBI GATnn'nnATC Cuts at: 0 96 1299 Size: 96 1203 BsaWI w'CCGG_w Cuts at: 0 878 998 1299 Size: 878 120 301 Fragments arranged by size: 878 301 120 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 596 626 1094 1124 1299 Size: 596 30 468 30 175 Fragments arranged by size: 596 468 175 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 373 838 1060 1299 Size: 373 465 222 239 Fragments arranged by size: 465 373 239 222 BseYI C'CCAG_C Cuts at: 0 1169 1257 1299 Size: 1169 88 42 Fragments arranged by size: 1169 88 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 1299 Size: 352 947 BsiHKAI G_wGCw'C Cuts at: 0 697 1299 Size: 697 602 BsmBI CGTCTCn'nnnn_ Cuts at: 0 259 1299 Size: 259 1040 Bsp1286I G_dGCh'C Cuts at: 0 222 697 1071 1241 1257 1299 Size: 222 475 374 170 16 42 Fragments arranged by size: 475 374 222 170 42 16 BspEI T'CCGG_A Cuts at: 0 878 1299 Size: 878 421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1039 1299 Size: 1039 260 BsrDI GCAATG_nn' Cuts at: 0 914 967 1299 Size: 914 53 332 Fragments arranged by size: 914 332 53 BssSI C'ACGA_G Cuts at: 0 196 280 1299 Size: 196 84 1019 Fragments arranged by size: 1019 196 84 BstXI CCAn_nnnn'nTGG Cuts at: 0 659 1299 Size: 659 640 BstYI r'GATC_y Cuts at: 0 248 266 495 1173 1299 Size: 248 18 229 678 126 Fragments arranged by size: 678 248 229 126 18 Bsu36I CC'TnA_GG Cuts at: 0 506 1299 Size: 506 793 BtgI C'CryG_G Cuts at: 0 120 1109 1299 Size: 120 989 190 Fragments arranged by size: 989 190 120 BtsI GCAGTG_nn' Cuts at: 0 695 1299 Size: 695 604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 572 607 757 792 1299 Size: 572 35 150 35 507 Fragments arranged by size: 572 507 150 35 35 EarI CTCTTCn'nnn_ Cuts at: 0 71 431 1299 Size: 71 360 868 Fragments arranged by size: 868 360 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 144 189 292 307 762 976 1072 Size: 71 73 45 103 15 455 214 96 Cuts at: 1072 1199 1299 Size: 127 100 Fragments arranged by size: 455 214 127 103 100 96 73 71 45 15 EcoO109I rG'GnC_Cy Cuts at: 0 446 1056 1237 1253 1254 1299 Size: 446 610 181 16 1 45 Fragments arranged by size: 610 446 181 45 16 1 HaeII r_GCGC'y Cuts at: 0 159 1299 Size: 159 1140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 109 141 499 531 1299 Size: 109 32 358 32 768 Fragments arranged by size: 768 358 109 32 32 KpnI G_GTAC'C Cuts at: 0 1031 1299 Size: 1031 268 MslI CAynn'nnrTG Cuts at: 0 566 796 1299 Size: 566 230 503 Fragments arranged by size: 566 503 230 MspA1I CmG'CkG Cuts at: 0 187 483 1299 Size: 187 296 816 Fragments arranged by size: 816 296 187 NspI r_CATG'y Cuts at: 0 545 1299 Size: 545 754 PasI CC'CwG_GG Cuts at: 0 1233 1299 Size: 1233 66 PflMI CCAn_nnn'nTGG Cuts at: 0 107 1299 Size: 107 1192 PfoI T'CCnGG_A Cuts at: 0 269 1299 Size: 269 1030 PsiI TTA'TAA Cuts at: 0 676 1299 Size: 676 623 PspOMI G'GGCC_C Cuts at: 0 1237 1253 1299 Size: 1237 16 46 Fragments arranged by size: 1237 46 16 PstI C_TGCA'G Cuts at: 0 408 488 702 1299 Size: 408 80 214 597 Fragments arranged by size: 597 408 214 80 PvuII CAG'CTG Cuts at: 0 187 483 1299 Size: 187 296 816 Fragments arranged by size: 816 296 187 SexAI A'CCwGG_T Cuts at: 0 1023 1299 Size: 1023 276 SfcI C'TryA_G Cuts at: 0 404 484 698 969 1299 Size: 404 80 214 271 330 Fragments arranged by size: 404 330 271 214 80 StuI AGG'CCT Cuts at: 0 145 202 1299 Size: 145 57 1097 Fragments arranged by size: 1097 145 57 StyI C'CwwG_G Cuts at: 0 310 371 1299 Size: 310 61 928 Fragments arranged by size: 928 310 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 318 1034 1299 Size: 318 716 265 Fragments arranged by size: 716 318 265 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1094 1126 1299 Size: 1094 32 173 Fragments arranged by size: 1094 173 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 349 497 837 1276 1299 Size: 349 148 340 439 23 Fragments arranged by size: 439 349 340 148 23 Enzymes that do cut: AccI Acc65I AcuI AfeI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI EarI Eco57MI EcoO109I HaeII Hin4I KpnI MslI MspA1I NspI PasI PflMI PfoI PsiI PspOMI PstI PvuII SexAI SfcI StuI StyI TaqII TstI XcmI Enzymes that do not cut: AarI AatII AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI