Restriction map for
pFN21AE5569
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AccI AclI AcsI AflII AgeI
AhdI AocI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
Eam1105I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KspI MaeI
MfeI MluI MluNI MscI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 514 GACGTC GACGT^C
Acc65I 1152 GGTACC G^GTACC
AccIII 551 TCCGGA T^CCGGA
AflIII 26 ACACGT A^C[GA][CT]GT
Alw44I 19 GTGCAC G^TGCAC
AlwI 549 GATCC GGATC(4/5)
AlwNI 835 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1317 AGCGCT AGC^GCT
ApaLI 19 GTGCAC G^TGCAC
Asp718I 1152 GGTACC G^GTACC
AvaII 990 GGACC G^G[AT]CC
BbeI 1234 GGCGCC GGCGC^C
BcgI 467 GCAAGTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 284 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 87 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 415 GAGACC GGTCTC(1/5)
BsaWI 551 TCCGGA [AT]^CCGG[AT]
BseAI 551 TCCGGA T^CCGGA
BsmAI 415 GAGAC GTCTC(1/5)
Bsp1407I 719 TGTACA T^GTACA
BspEI 551 TCCGGA T^CCGGA
BspMI 1106 ACCTGC ACCTGC(4/8)
BsrFI 1248 GCCGGC [GA]^CCGG[CT]
BsrGI 719 TGTACA T^GTACA
BssHII 336 GCGCGC G^CGCGC
BstYI 548 AGATCC [GA]^GATC[CT]
Cfr10I 1248 GCCGGC [GA]^CCGG[CT]
DrdI 514 GACGTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1317 AGCGCT AGC^GCT
EcoNI 519 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1234 GGCGCC G^GCGCC
KpnI 1152 GGTACC GGTAC^C
MaeIII 1229 GTCAC ^GT[ACGT]AC
MamI 87 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 548 AGATCC [GA]^GATC[CT]
MroI 551 TCCGGA T^CCGGA
NaeI 1248 GCCGGC GCC^GGC
NarI 1234 GGCGCC GG^CGCC
NcoI 218 CCATGG C^CATGG
NgoMIV 1248 GCCGGC G^CCGGC
SacI 43 GAGCTC GAGCT^C
SapI 928 GAAGAGC GCTCTTC(1/4)
SfaNI 793 GATGC GCATC(5/9)
SfoI 1234 GGCGCC GGC^GCC
SspBI 719 TGTACA T^GTACA
StuI 647 AGGCCT AGG^CCT
Tsp45I 1229 GTCAC ^GT[GC]AC
Tsp509I 801 AATT ^AATT
TspRI 1201 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 209 CCAAGAGCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 548 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 1152 GGTACC G^G[CT][GA]CC
1234 GGCGCC
BlpI 306 GCTCAGC GC^T[ACGT]AGC
1137 GCTCAGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
1137 GCTCAGC
BsgI 175 GTGCAG GTGCAG(16/14)
655 GTGCAG
BsiEI 341 CGGCCG CG[GA][CT]^CG
433 CGGCCG
BsmFI 380 GGGAC GGGAC(10/14)
989 GGGAC
BsrBI 430 GAGCGG CCGCTC(-3/-3)
899 GAGCGG
BstXI 92 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
443 CCATCCACCTGG
CelII 306 GCTCAGC GC^T[ACGT]AGC
1137 GCTCAGC
EaeI 341 CGGCCG [CT]^GGCC[GA]
433 CGGCCG
EagI 341 CGGCCG C^GGCCG
433 CGGCCG
EarI 928 GAAGAG CTCTTC(1/4)
955 GAAGAG
EclXI 341 CGGCCG C^GGCCG
433 CGGCCG
Eco52I 341 CGGCCG C^GGCCG
433 CGGCCG
HgaI 397 GACGC GACGC(5/10)
1227 GCGTC
HindIII 829 AAGCTT A^AGCTT
961 AAGCTT
HphI 150 GGTGA GGTGA(8/7)
80 TCACC
Ksp632I 928 GAAGAG CTCTTC(1/4)
955 GAAGAG
MaeII 28 ACGT A^CGT
515 ACGT
MseI 803 TTAA T^TAA
1279 TTAA
TfiI 772 GATTC G^A[AT]TC
1008 GAATC
Tru9I 803 TTAA T^TAA
1279 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SmaI StyI TaqI TseI
TthHB8I XmaCI XmaI