(Circular) (Six-base) MAPSORT of: pFN21AE5569.seq Check: 1380 from: 1 to: 6043 pFN21AE5569 6043 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2523 3853 278 Size: 53 83 186 936 987 1330 2468 Fragments arranged by size: 2468 1330 987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3157 3364 3157 Size: 207 5836 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2141 2189 2354 2507 2608 2822 4106 Size: 464 45 48 165 153 101 214 1284 Cuts at: 4106 5490 5758 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1284 464 268 214 165 153 101 48 45 AfeI AGC'GCT Cuts at: 3324 3324 Size: 6043 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 2031 4942 5919 1203 Size: 828 2911 977 1327 Fragments arranged by size: 2911 1327 977 828 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 2793 1166 Size: 1627 4416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3110 1184 Size: 1926 4117 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3101 3133 3101 Size: 32 6011 AlwNI CAG_nnn'CTG Cuts at: 2845 3786 5358 2845 Size: 941 1572 3530 Fragments arranged by size: 3530 1572 941 ApaLI G'TGCA_C Cuts at: 2024 4100 5256 2024 Size: 2076 1156 2811 Fragments arranged by size: 2811 2076 1156 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3161 3193 3309 3339 3368 1870 Size: 73 1218 32 116 30 29 4545 Fragments arranged by size: 4545 1218 116 73 32 30 29 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 3239 3364 4819 618 Size: 325 217 627 1370 82 125 1455 1842 Fragments arranged by size: 1842 1455 1370 627 325 217 125 82 BanII G_rGCy'C Cuts at: 1391 2052 2501 3170 3362 1391 Size: 661 449 669 192 4072 Fragments arranged by size: 4072 669 661 449 192 BbeI G_GCGC'C Cuts at: 3243 3243 Size: 6043 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2461 2495 4234 4268 1903 Size: 34 524 34 1739 34 3678 Fragments arranged by size: 3678 1739 524 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 4654 136 Size: 107 122 71 631 605 623 2359 1525 Fragments arranged by size: 2359 1525 631 623 605 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 2312 3143 3460 2312 Size: 831 317 4895 Fragments arranged by size: 4895 831 317 Bme1580I G_kGCm'C Cuts at: 1790 2028 2640 3212 4104 5260 1790 Size: 238 612 572 892 1156 2573 Fragments arranged by size: 2573 1156 892 612 572 238 BmgBI CAC'GTC Cuts at: 1318 2034 1318 Size: 716 5327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 2392 3774 Size: 416 53 83 399 24 741 234 1382 Cuts at: 3774 4732 442 Size: 958 1753 Fragments arranged by size: 1753 1382 958 741 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2515 2515 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2843 2867 2960 3047 3098 4703 1239 Size: 1238 366 24 93 87 51 1605 2579 Fragments arranged by size: 2579 1605 1238 366 93 87 51 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 4706 882 Size: 617 915 2292 2219 Fragments arranged by size: 2292 2219 915 617 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 2096 6037 2096 Size: 3941 2102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2403 2520 3232 3240 Size: 53 83 186 936 870 117 712 8 Cuts at: 3240 3850 4232 4867 275 Size: 610 382 635 1451 Fragments arranged by size: 1451 936 870 712 635 610 382 186 117 83 53 8 BsaWI w'CCGG_w Cuts at: 2556 4476 4874 5148 5295 5706 2556 Size: 1920 398 274 147 411 2893 Fragments arranged by size: 2893 1920 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2372 2402 1178 Size: 30 165 30 777 30 162 30 4819 Fragments arranged by size: 4819 777 165 162 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 2906 3059 3095 1186 Size: 1423 297 153 36 4134 Fragments arranged by size: 4134 1423 297 153 36 BseYI C'CCAG_C Cuts at: 2510 5246 5742 2510 Size: 2736 496 2811 Fragments arranged by size: 2811 2736 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 2681 1163 Size: 573 465 480 4525 Fragments arranged by size: 4525 573 480 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2349 2441 3493 4254 5282 1540 Size: 275 186 348 92 1052 761 1028 2301 Fragments arranged by size: 2301 1052 1028 761 348 275 186 92 BsiHKAI G_wGCw'C Cuts at: 2028 2052 2222 4104 4189 5260 2028 Size: 24 170 1882 85 1071 2811 Fragments arranged by size: 2811 1882 1071 170 85 24 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2052 2222 2501 2640 3170 3212 Size: 399 238 24 170 279 139 530 42 Cuts at: 3212 3362 4104 4189 5260 1391 Size: 150 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 399 279 238 170 150 139 85 42 24 BspEI T'CCGG_A Cuts at: 2556 5706 2556 Size: 3150 2893 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3120 3398 844 Size: 2276 278 3489 Fragments arranged by size: 3489 2276 278 BsrBI CCG'CTC Cuts at: 1394 1823 2437 2906 3930 1394 Size: 429 614 469 1024 3507 Fragments arranged by size: 3507 1024 614 469 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3253 4687 4874 1700 Size: 110 1443 1434 187 2869 Fragments arranged by size: 2869 1443 1434 187 110 BsrGI T'GTAC_A Cuts at: 96 2724 96 Size: 2628 3415 BssHII G'CGCG_C Cuts at: 1925 2341 1925 Size: 416 5627 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstXI CCAn_nnnn'nTGG Cuts at: 2104 2455 2104 Size: 351 5692 BstYI r'GATC_y Cuts at: 1972 2553 3373 3740 4125 4142 5594 5680 5692 Size: 581 820 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 820 581 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2223 2748 513 Size: 552 939 39 180 525 3808 Fragments arranged by size: 3808 939 552 525 180 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3115 487 Size: 683 56 1889 3415 Fragments arranged by size: 3415 1889 683 56 BtsI GCAGTG_nn' Cuts at: 3559 4353 4373 3559 Size: 794 20 5229 Fragments arranged by size: 5229 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 5050 809 Size: 507 1209 2525 1802 Fragments arranged by size: 2525 1802 1209 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2346 2438 3490 Size: 54 1425 50 275 18 516 92 1052 Cuts at: 3490 4379 6008 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1052 889 516 275 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2346 2438 3490 1537 Size: 275 534 92 1052 4090 Fragments arranged by size: 4090 1052 534 275 92 EarI CTCTTCn'nnn_ Cuts at: 1353 2928 2955 3973 1353 Size: 1575 27 1018 3423 Fragments arranged by size: 3423 1575 1018 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3056 4630 5004 5150 387 Size: 1064 138 1467 1574 374 146 1280 Fragments arranged by size: 1574 1467 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2050 2050 Size: 6043 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2141 2189 2354 2477 2507 2608 Size: 393 464 45 48 165 123 30 101 Cuts at: 2608 2822 2843 2867 2960 3047 3098 4106 4703 Size: 214 21 24 93 87 51 1008 597 Cuts at: 4703 5490 5758 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 787 597 464 393 268 214 165 123 101 93 87 51 48 45 30 24 21 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 6043 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2380 2984 2380 Size: 604 5439 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 2703 3243 3326 5190 1678 Size: 1025 540 83 1864 2531 Fragments arranged by size: 2531 1864 1025 540 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 3286 3318 4684 Size: 32 135 32 800 32 882 32 1366 Cuts at: 4684 4716 4758 4790 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1366 882 800 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 2834 2966 748 Size: 2086 132 3825 Fragments arranged by size: 3825 2086 132 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KasI G'GCGC_C Cuts at: 3239 3239 Size: 6043 KpnI G_GTAC'C Cuts at: 3161 3368 3161 Size: 207 5836 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2349 5157 5341 1305 Size: 864 180 2808 184 2007 Fragments arranged by size: 2808 2007 864 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4221 Fragments arranged by size: 4221 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2102 2573 2793 4001 4360 4519 Size: 648 924 12 471 220 1208 359 159 Cuts at: 4519 518 Size: 2042 Fragments arranged by size: 2042 1208 924 648 471 359 220 159 12 MspA1I CmG'CkG Cuts at: 1606 2012 2119 2316 2821 3058 3263 3459 4136 Size: 406 107 197 505 237 205 196 677 Cuts at: 4136 5284 5529 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 505 406 245 237 205 197 196 107 NaeI GCC'GGC Cuts at: 3255 3255 Size: 6043 NarI GG'CG_CC Cuts at: 3240 3240 Size: 6043 NcoI C'CATG_G Cuts at: 513 1065 2004 2223 513 Size: 552 939 219 4333 Fragments arranged by size: 4333 939 552 219 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NgoMIV G'CCGG_C Cuts at: 3253 3253 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3401 4946 1132 Size: 97 2172 1545 2229 Fragments arranged by size: 2229 2172 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PstI C_TGCA'G Cuts at: 830 3039 3063 3117 3179 3262 3395 830 Size: 2209 24 54 62 83 133 3478 Fragments arranged by size: 3478 2209 133 83 62 54 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2119 2821 3058 3263 2119 Size: 702 237 205 4899 Fragments arranged by size: 4899 702 237 205 SacI G_AGCT'C Cuts at: 2052 2052 Size: 6043 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SapI GCTCTTCn'nnn_ Cuts at: 2928 2928 Size: 6043 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 3035 3059 3113 3175 3258 3264 3391 Size: 219 1990 24 54 62 83 6 127 Cuts at: 3391 4526 5207 5398 826 Size: 1135 681 191 1471 Fragments arranged by size: 1990 1471 1135 681 219 191 127 83 62 54 24 6 SfoI GGC'GCC Cuts at: 3241 3241 Size: 6043 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3163 3195 3311 3370 1872 Size: 1291 32 116 59 4545 Fragments arranged by size: 4545 1291 116 59 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SrfI GCCC'GGGC Cuts at: 3195 3195 Size: 6043 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 2654 1517 Size: 1137 4906 StyI C'CwwG_G Cuts at: 513 1065 2004 2223 2406 3153 3482 513 Size: 552 939 219 183 747 329 3074 Fragments arranged by size: 3074 939 747 552 329 219 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4070 4087 4240 4425 4870 325 Size: 1255 2490 17 153 185 445 1498 Fragments arranged by size: 2490 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 2724 4289 Size: 274 80 33 51 1443 696 51 1565 Cuts at: 4289 6001 96 Size: 1712 138 Fragments arranged by size: 1712 1565 1443 696 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 6003 6035 2180 Size: 32 3791 32 2188 Fragments arranged by size: 3791 2188 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2221 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3161 3193 3309 3368 1870 Size: 1291 32 116 59 4545 Fragments arranged by size: 4545 1291 116 59 32 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2521 3851 276 Size: 53 83 186 936 987 1330 2468 Fragments arranged by size: 2468 1330 987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI