(Linear) (Six-base) MAPSORT of: KIEE5569.seq Check: 8746 from: 1 to: 1338 KIEE5569.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AatII G_ACGT'C Cuts at: 0 518 1338 Size: 518 820 Acc65I G'GTAC_C Cuts at: 0 1152 1338 Size: 1152 186 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 136 184 349 502 603 817 1338 Size: 91 45 48 165 153 101 214 521 Fragments arranged by size: 521 214 165 153 101 91 48 45 AfeI AGC'GCT Cuts at: 0 1319 1338 Size: 1319 19 AflIII A'CryG_T Cuts at: 0 26 1338 Size: 26 1312 AleI CACnn'nnGTG Cuts at: 0 788 1338 Size: 788 550 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1105 1338 Size: 1105 233 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1096 1128 1338 Size: 1096 32 210 Fragments arranged by size: 1096 210 32 AlwNI CAG_nnn'CTG Cuts at: 0 840 1338 Size: 840 498 ApaLI G'TGCA_C Cuts at: 0 19 1338 Size: 19 1319 AvaI C'yCGr_G Cuts at: 0 1156 1188 1304 1338 Size: 1156 32 116 34 Fragments arranged by size: 1156 116 34 32 BanI G'GyrC_C Cuts at: 0 1152 1234 1338 Size: 1152 82 104 Fragments arranged by size: 1152 104 82 BanII G_rGCy'C Cuts at: 0 47 496 1165 1338 Size: 47 449 669 173 Fragments arranged by size: 669 449 173 47 BbeI G_GCGC'C Cuts at: 0 1238 1338 Size: 1238 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 456 490 1338 Size: 456 34 848 Fragments arranged by size: 848 456 34 BglI GCCn_nnn'nGGC Cuts at: 0 290 1338 Size: 290 1048 BlpI GC'TnA_GC Cuts at: 0 307 1138 1338 Size: 307 831 200 Fragments arranged by size: 831 307 200 Bme1580I G_kGCm'C Cuts at: 0 23 635 1207 1338 Size: 23 612 572 131 Fragments arranged by size: 612 572 131 23 BmgBI CAC'GTC Cuts at: 0 29 1338 Size: 29 1309 BmrI ACTGGGnnnn_n' Cuts at: 0 153 387 1338 Size: 153 234 951 Fragments arranged by size: 951 234 153 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 510 1338 Size: 510 828 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 838 862 955 1042 1093 1338 Size: 472 366 24 93 87 51 245 Fragments arranged by size: 472 366 245 93 87 51 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 409 1338 Size: 409 929 BsaBI GATnn'nnATC Cuts at: 0 91 1338 Size: 91 1247 BsaHI Gr'CG_yC Cuts at: 0 398 515 1227 1235 1338 Size: 398 117 712 8 103 Fragments arranged by size: 712 398 117 103 8 BsaWI w'CCGG_w Cuts at: 0 551 1338 Size: 551 787 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 175 205 367 397 1338 Size: 175 30 162 30 941 Fragments arranged by size: 941 175 162 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 604 901 1054 1090 1338 Size: 604 297 153 36 248 Fragments arranged by size: 604 297 248 153 36 BseYI C'CCAG_C Cuts at: 0 505 1338 Size: 505 833 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 676 1338 Size: 196 480 662 Fragments arranged by size: 662 480 196 BsiEI CG_ry'CG Cuts at: 0 344 436 1338 Size: 344 92 902 Fragments arranged by size: 902 344 92 BsiHKAI G_wGCw'C Cuts at: 0 23 47 217 1338 Size: 23 24 170 1121 Fragments arranged by size: 1121 170 24 23 Bsp1286I G_dGCh'C Cuts at: 0 23 47 217 496 635 1165 1207 1338 Size: 23 24 170 279 139 530 42 131 Fragments arranged by size: 530 279 170 139 131 42 24 23 BspEI T'CCGG_A Cuts at: 0 551 1338 Size: 551 787 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1115 1338 Size: 1115 223 BsrBI CCG'CTC Cuts at: 0 432 901 1338 Size: 432 469 437 Fragments arranged by size: 469 437 432 BsrFI r'CCGG_y Cuts at: 0 1248 1338 Size: 1248 90 BsrGI T'GTAC_A Cuts at: 0 719 1338 Size: 719 619 BssHII G'CGCG_C Cuts at: 0 336 1338 Size: 336 1002 BstXI CCAn_nnnn'nTGG Cuts at: 0 99 450 1338 Size: 99 351 888 Fragments arranged by size: 888 351 99 BstYI r'GATC_y Cuts at: 0 548 1338 Size: 548 790 BtgI C'CryG_G Cuts at: 0 38 218 743 1338 Size: 38 180 525 595 Fragments arranged by size: 595 525 180 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1110 1338 Size: 1110 228 DrdI GACnn_nn'nnGTC Cuts at: 0 520 1338 Size: 520 818 EaeI y'GGCC_r Cuts at: 0 341 433 1338 Size: 341 92 905 Fragments arranged by size: 905 341 92 EagI C'GGCC_G Cuts at: 0 341 433 1338 Size: 341 92 905 Fragments arranged by size: 905 341 92 EarI CTCTTCn'nnn_ Cuts at: 0 923 950 1338 Size: 923 27 388 Fragments arranged by size: 923 388 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1051 1338 Size: 1051 287 EcoICRI GAG'CTC Cuts at: 0 45 1338 Size: 45 1293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 136 184 349 472 502 603 817 Size: 91 45 48 165 123 30 101 214 Cuts at: 817 838 862 955 1042 1093 1338 Size: 21 24 93 87 51 245 Fragments arranged by size: 245 214 165 123 101 93 91 87 51 48 45 30 24 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 523 1338 Size: 523 815 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 375 979 1338 Size: 375 604 359 Fragments arranged by size: 604 375 359 HaeII r_GCGC'y Cuts at: 0 698 1238 1321 1338 Size: 698 540 83 17 Fragments arranged by size: 698 540 83 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 367 399 1281 1313 1338 Size: 367 32 882 32 25 Fragments arranged by size: 882 367 32 32 25 HindIII A'AGCT_T Cuts at: 0 829 961 1338 Size: 829 132 377 Fragments arranged by size: 829 377 132 KasI G'GCGC_C Cuts at: 0 1234 1338 Size: 1234 104 KpnI G_GTAC'C Cuts at: 0 1156 1338 Size: 1156 182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 164 344 1338 Size: 164 180 994 Fragments arranged by size: 994 180 164 MslI CAynn'nnrTG Cuts at: 0 85 97 568 788 1338 Size: 85 12 471 220 550 Fragments arranged by size: 550 471 220 85 12 MspA1I CmG'CkG Cuts at: 0 7 114 311 816 1053 1258 1338 Size: 7 107 197 505 237 205 80 Fragments arranged by size: 505 237 205 197 107 80 7 NaeI GCC'GGC Cuts at: 0 1250 1338 Size: 1250 88 NarI GG'CG_CC Cuts at: 0 1235 1338 Size: 1235 103 NcoI C'CATG_G Cuts at: 0 218 1338 Size: 218 1120 NgoMIV G'CCGG_C Cuts at: 0 1248 1338 Size: 1248 90 PstI C_TGCA'G Cuts at: 0 1034 1058 1112 1174 1257 1338 Size: 1034 24 54 62 83 81 Fragments arranged by size: 1034 83 81 62 54 24 PvuII CAG'CTG Cuts at: 0 114 816 1053 1258 1338 Size: 114 702 237 205 80 Fragments arranged by size: 702 237 205 114 80 SacI G_AGCT'C Cuts at: 0 47 1338 Size: 47 1291 SapI GCTCTTCn'nnn_ Cuts at: 0 923 1338 Size: 923 415 SfcI C'TryA_G Cuts at: 0 1030 1054 1108 1170 1253 1259 1338 Size: 1030 24 54 62 83 6 79 Fragments arranged by size: 1030 83 79 62 54 24 6 SfoI GGC'GCC Cuts at: 0 1236 1338 Size: 1236 102 SmaI CCC'GGG Cuts at: 0 1158 1190 1306 1338 Size: 1158 32 116 32 Fragments arranged by size: 1158 116 32 32 SrfI GCCC'GGGC Cuts at: 0 1190 1338 Size: 1190 148 StuI AGG'CCT Cuts at: 0 649 1338 Size: 649 689 StyI C'CwwG_G Cuts at: 0 218 401 1148 1338 Size: 218 183 747 190 Fragments arranged by size: 747 218 190 183 TatI w'GTAC_w Cuts at: 0 668 719 1338 Size: 668 51 619 Fragments arranged by size: 668 619 51 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 175 207 1338 Size: 175 32 1131 Fragments arranged by size: 1131 175 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 216 1338 Size: 216 1122 XmaI C'CCGG_G Cuts at: 0 1156 1188 1304 1338 Size: 1156 32 116 34 Fragments arranged by size: 1156 116 34 32 ZraI GAC'GTC Cuts at: 0 516 1338 Size: 516 822 Enzymes that do cut: AatII Acc65I AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaLI AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BstXI BstYI BtgI BtgZI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI FalI HaeII Hin4I HindIII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PstI PvuII SacI SapI SfcI SfoI SmaI SrfI StuI StyI TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI AclI AflII AgeI AhdI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI Bpu10I BpuEI BsaAI BsiWI BsmI BsmBI BspHI BsrDI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HpaI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TaqII Tth111I XbaI XhoI XmnI