Restriction map for
pFN21AE6671
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 228 CGCG CG^CG
AciI 713 GCGG CCGC(-3/-1)
AlwNI 256 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 203 GGGCCC GGGCC^C
Asp700I 499 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 706 GAGCAC G[AT]GC[AT]^C
AspI 175 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 845 CCCGAG C^[CT]CG[GA]G
BamHI 105 GGATCC G^GATCC
BbvCI 261 GCTGAGG CCTCAGC(-5/-2)
BclI 234 TGATCA T^GATCA
BcnI 65 CCCGG CC^[GC]GG
BfaI 826 CTAG C^TAG
BsaAI 145 TACGTG [CT]AC^GT[GA]
BsiHKAI 706 GAGCAC G[AT]GC[AT]^C
BsoBI 845 CCCGAG C^[CT]CG[GA]G
BstAPI 372 GCATGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 228 CGCG CG^CG
BstYI 105 GGATCC [GA]^GATC[CT]
EarI 523 CTCTTC CTCTTC(1/4)
FbaI 234 TGATCA T^GATCA
HaeII 299 AGCGCC [GA]GCGC^[CT]
HapII 66 CCGG C^CGG
HgaI 69 GACGC GACGC(5/10)
HpaII 66 CCGG C^CGG
Ksp632I 523 CTCTTC CTCTTC(1/4)
MaeI 826 CTAG C^TAG
MaeII 146 ACGT A^CGT
MfeI 659 CAATTG C^AATTG
MflI 105 GGATCC [GA]^GATC[CT]
MseI 43 TTAA T^TAA
MspA1I 256 CAGCTG C[AC]G^C[GT]G
MspI 66 CCGG C^CGG
MunI 659 CAATTG C^AATTG
MvnI 228 CGCG CG^CG
NciI 65 CCCGG CC^[GC]GG
NdeI 170 CATATG CA^TATG
PflFI 175 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 151 GACTC GAGTC(4/5)
PvuII 256 CAGCTG CAG^CTG
SfaNI 655 GCATC GCATC(5/9)
SfcI 304 CTACAG C^T[GA][CT]AG
TaqI 885 TCGA T^CGA
Tru9I 43 TTAA T^TAA
Tsp45I 181 GTCAC ^GT[GC]AC
Tth111I 175 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 885 TCGA T^CGA
XcmI 199 CCACGGGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 105 GGATCC [GA]^GATC[CT]
XmnI 499 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 127 GAATTT [GA]^AATT[CT]
557 AAATTT
AfaI 144 GTAC GT^AC
249 GTAC
ApoI 127 GAATTT [GA]^AATT[CT]
557 AAATTT
BanII 203 GGGCCC G[GA]GC[CT]^C
469 GAGCCC
BsmAI 614 GAGAC GTCTC(1/5)
778 GTCTC
BsmFI 121 GTCCC GGGAC(10/14)
736 GTCCC
CfoI 229 GCGC GCG^C
300 GCGC
EcoT14I 114 CCATGG C^C[AT][AT]GG
208 CCATGG
HhaI 229 GCGC GCG^C
300 GCGC
HinP1I 229 GCGC G^CGC
300 GCGC
HphI 48 GGTGA GGTGA(8/7)
164 TCACC
MslI 33 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
709 CACTGCGGTG
NcoI 114 CCATGG C^CATGG
208 CCATGG
PpuMI 119 GGGTCCC [GA]G^G[AT]CC[CT]
734 AGGTCCC
RsaI 144 GTAC GT^AC
249 GTAC
StyI 114 CCATGG C^C[AT][AT]GG
208 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AlwI AvaII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TfiI
TseI Tsp509I TspRI