Restriction map for
pFN21AE6671
(Linear) (Six-base) MAPSORT of: KIEE6671.seq Check: 4249 from: 1 to: 888
KIEE6671.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 715 888
Size: 316 399 173
Fragments arranged by size:
399 316 173
AleI CACnn'nnGTG
Cuts at: 0 713 888
Size: 713 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 806 838 888
Size: 806 32 50
Fragments arranged by size:
806 50 32
AlwNI CAG_nnn'CTG
Cuts at: 0 261 888
Size: 261 627
ApaI G_GGCC'C
Cuts at: 0 207 888
Size: 207 681
ApoI r'AATT_y
Cuts at: 0 127 557 888
Size: 127 430 331
Fragments arranged by size:
430 331 127
AvaI C'yCGr_G
Cuts at: 0 845 888
Size: 845 43
BamHI G'GATC_C
Cuts at: 0 105 888
Size: 105 783
BanII G_rGCy'C
Cuts at: 0 207 473 888
Size: 207 266 415
Fragments arranged by size:
415 266 207
BbvCI CC'TCA_GC
Cuts at: 0 262 888
Size: 262 626
BclI T'GATC_A
Cuts at: 0 234 888
Size: 234 654
Bme1580I G_kGCm'C
Cuts at: 0 207 888
Size: 207 681
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 620 888
Size: 249 371 268
Fragments arranged by size:
371 268 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 269 888
Size: 269 619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 833 852 888
Size: 457 376 19 36
Fragments arranged by size:
457 376 36 19
Bpu10I CC'TnA_GC
Cuts at: 0 262 888
Size: 262 626
BsaAI yAC'GTr
Cuts at: 0 147 888
Size: 147 741
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 806 836 888
Size: 676 30 100 30 52
Fragments arranged by size:
676 100 52 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 710 888
Size: 710 178
Bsp1286I G_dGCh'C
Cuts at: 0 207 473 710 888
Size: 207 266 237 178
Fragments arranged by size:
266 237 207 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 378 888
Size: 378 510
BstYI r'GATC_y
Cuts at: 0 105 888
Size: 105 783
BtgI C'CryG_G
Cuts at: 0 114 199 208 888
Size: 114 85 9 680
Fragments arranged by size:
680 114 85 9
BtsI GCAGTG_nn'
Cuts at: 0 708 888
Size: 708 180
EarI CTCTTCn'nnn_
Cuts at: 0 529 888
Size: 529 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 457 715 833 852 888
Size: 316 141 258 118 19 36
Fragments arranged by size:
316 258 141 118 36 19
EcoO109I rG'GnC_Cy
Cuts at: 0 120 204 735 842 888
Size: 120 84 531 107 46
Fragments arranged by size:
531 120 107 84 46
HaeII r_GCGC'y
Cuts at: 0 303 888
Size: 303 585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 888
Size: 60 32 796
Fragments arranged by size:
796 60 32
MfeI C'AATT_G
Cuts at: 0 659 888
Size: 659 229
MslI CAynn'nnrTG
Cuts at: 0 37 713 888
Size: 37 676 175
Fragments arranged by size:
676 175 37
MspA1I CmG'CkG
Cuts at: 0 258 888
Size: 258 630
NcoI C'CATG_G
Cuts at: 0 114 208 888
Size: 114 94 680
Fragments arranged by size:
680 114 94
NdeI CA'TA_TG
Cuts at: 0 171 888
Size: 171 717
PfoI T'CCnGG_A
Cuts at: 0 64 434 888
Size: 64 370 454
Fragments arranged by size:
454 370 64
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 806 838 888
Size: 806 32 50
Fragments arranged by size:
806 50 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 735 888
Size: 120 615 153
Fragments arranged by size:
615 153 120
PspOMI G'GGCC_C
Cuts at: 0 203 888
Size: 203 685
PvuII CAG'CTG
Cuts at: 0 258 888
Size: 258 630
SanDI GG'GwC_CC
Cuts at: 0 120 888
Size: 120 768
SfcI C'TryA_G
Cuts at: 0 304 888
Size: 304 584
StyI C'CwwG_G
Cuts at: 0 114 208 888
Size: 114 94 680
Fragments arranged by size:
680 114 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 888
Size: 46 32 810
Fragments arranged by size:
810 46 32
Tth111I GACn'n_nGTC
Cuts at: 0 178 888
Size: 178 710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 206 888
Size: 206 682
XmnI GAAnn'nnTTC
Cuts at: 0 503 888
Size: 503 385
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI ApoI AvaI BamHI
BanII BbvCI BclI Bme1580I BmrI BplI BpmI Bpu10I
BsaAI BsaXI BsiHKAI Bsp1286I BstAPI BstYI BtgI BtsI
EarI Eco57MI EcoO109I HaeII Hin4I MfeI MslI MspA1I
NcoI NdeI PfoI PpiI PpuMI PspOMI PvuII SanDI
SfcI StyI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI XbaI XhoI XmaI ZraI